The effect of different temperatures on the fatty acid composition of <i>Rhizobium leguminosarum</i> bv. <i>viciae</i> in the faba bean symbiosis

Théberge, Marie-Claude; Prévost, Danielle; Chalifour, François-P.

doi:10.1111/j.1469-8137.1996.tb04931.x

Cited by 28 publications

(29 citation statements)

References 37 publications

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“…Monounsaturated 18∶1, along with the saturated 16∶0 and 18∶0 fatty acids are the major lipids found in rhizobia grown at optimal temperature [34]–[37]. The fatty acids identified by GC-MS which showed significant (P≤0.05; FC ≥2) concentration shifts at low temperatures are shown in Figure 7 .…”

Section: Resultsmentioning

confidence: 99%

Metabolomic Analysis of Cold Acclimation of Arctic Mesorhizobium sp. Strain N33

et al. 2013

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Arctic Mesorhizobium sp. N33 isolated from nodules of Oxytropis arctobia in Canada’s eastern Arctic has a growth temperature range from 0°C to 30°C and is a well-known cold-adapted rhizobia. The key molecular mechanisms underlying cold adaptation in Arctic rhizobia remains totally unknown. Since the concentration and contents of metabolites are closely related to stress adaptation, we applied GC-MS and NMR to identify and quantify fatty acids and water soluble compounds possibly related to low temperature acclimation in strain N33. Bacterial cells were grown at three different growing temperatures (4°C, 10°C and 21°C). Cells from 21°C were also cold-exposed to 4°C for different times (2, 4, 8, 60 and 240 minutes). We identified that poly-unsaturated linoleic acids 18∶2 (9, 12) & 18∶2 (6, 9) were more abundant in cells growing at 4 or 10°C, than in cells cultivated at 21°C. The mono-unsaturated phospho/neutral fatty acids myristoleic acid 14∶1(11) were the most significantly overexpressed (45-fold) after 1hour of exposure to 4°C. As reported in the literature, these fatty acids play important roles in cold adaptability by supplying cell membrane fluidity, and by providing energy to cells. Analysis of water-soluble compounds revealed that isobutyrate, sarcosine, threonine and valine were more accumulated during exposure to 4°C. These metabolites might play a role in conferring cold acclimation to strain N33 at 4°C, probably by acting as cryoprotectants. Isobutyrate was highly upregulated (19.4-fold) during growth at 4°C, thus suggesting that this compound is a precursor for the cold-regulated fatty acids modification to low temperature adaptation.

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Section: Resultsmentioning

confidence: 99%

Metabolomic Analysis of Cold Acclimation of Arctic Mesorhizobium sp. Strain N33

et al. 2013

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“…Some studies describe the participation of FA in the adaptive response to changing environmental conditions (Th eberge et al 1996;Boumahdi et al 1999;Drouin et al 2000). However, little is known about the presence of an aerobic mechanism of FA synthesis and how both growth and shock temperature influence FA synthesis.…”

Section: Introductionmentioning

confidence: 99%

Monounsaturated fatty acid aerobic synthesis inBradyrhizobiumTAL1000 peanut-nodulating is affected by temperature

et al. 2013

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Aims The aim of this work was to clarify the mechanism of monounsaturated fatty acid (MUFA) synthesis in Bradyrhizobium TAL1000 and the effect of high temperature on this process. Methods and Results Bradyrhizobium TAL1000 was exposed to a high growth temperature and heat shock, and fatty acid composition and synthesis were tested. To determine the presence of a possible desaturase, a gene was identify and overexpressed in Escherichia coli. The desaturase expression was detected by RT‐PCR and Western blotting. In B. TAL1000, an aerobic mechanism for MUFA synthesis was detected. Desaturation was decreased by high growth temperature and by heat shock. Two hours of exposure to 37°C were required for the change in MUFA levels. A potential ∆9 desaturase gene was identified and successfully expressed in E. coli. A high growth temperature and not heat shock reduced transcript and protein desaturase levels in rhizobial strain. Conclusions In B. TAL1000, the anaerobic MUFA biosynthetic pathway is supplemented by an aerobic mechanism mediated by desaturase and is down‐regulated by temperature to maintain membrane fluidity under stressful conditions. Significance and Impact of the Study This knowledge will be useful for developing strategies to improve a sustainable practice of this bacterium under stress and to enhance the bioprocess for the inoculants' manufacture.

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“…In living organisms lipids, particularly polyunsaturated fatty acid (PUFA) components of cell membranes, are described as being extremely subject to environmental stress [12,13]. Stresses such as decreases in a w [14] or increases in temperature [15] are known to affect the fatty acid composition of bacteria. A number of factors, such as temperature, atmosphere, exposure to light and moisture influence the viability of freeze-dried cultures [16].…”

Section: Introductionmentioning

confidence: 99%

Survival of Freeze-dried Leuconostoc mesenteroides and Lactobacillus plantarum Related to Their Cellular Fatty Acids Composition during Storage

Coulibaly

Amenan

Lognay

et al. 2008

Appl Biochem Biotechnol

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Lactic acid bacteria strains Lactobacillus plantarum CWBI-B534 and Leuconostoc ssp. mesenteroïdes (L. mesenteroïdes) Kenya MRog2 were produced in bioreactor, concentrated, with or without cryoprotectants. In general, viable population did not change significantly after freeze-drying ( p>0.05). In most cases, viable population for cells added with cryoprotectants was significantly lower than those without ( p<0.05). Cellular fatty acids (CFAs) from the two strains in this study were analyzed before and after freeze-drying. Six CFAs were identified, namely, palmitic (C 16:0 ), palmitoleic (C 16:1 ), stearic (C 18:0 ), oleic (C 18:1 ), linoleic (C 18:2 ), and linolenic (C 18:3 ) acids were identified. Four of them, C 16:0 , C 16:1 , C 18:0 , and C 18:1 , make up more than 94% or 93% of the fatty acids in L. mesenteroides and L. plantarum, respectively, with another one, namely, C18:3, making a smaller (on average 5-6%, respectively) contribution. The C 18:2 contributed very small percentages (on average≤ 1%) to the total in each strain. C 16:0 had the highest proportion at most points relative to other fatty acids. Moisture content and water activity (a w ) increased significantly during the storage period. It was observed that C 16:1 /C 16:0 , C 18:0 /C 16:0 and C 18:1 /C 16:0 ratios for freeze-dried L. mesenteroides or L. plantarum, with or without cryoprotectants, did not change significantly during the storage period. According to the packaging mode and storage temperatures, C 18:2 /C 16:0 and C 18:3 /C 16:0 ratios for freeze-dried L. mesenteroides and L. plantarum with or without cryoprotectants decreased as the storage time increased. However, a higher C 18:2 /C 16:0 or C 18:3 /C 16:0 ratio for L. mesenteroides and L. plantarum was noted in the freeze-dried powder held at 4°C or under vacuum and in dark than at 20°C or in the presence of oxygen and light.Appl Biochem Biotechnol (2009) 157:70-84 DOI 10.1007/s12010-008-8240-

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The effect of different temperatures on the fatty acid composition of Rhizobium leguminosarum bv. viciae in the faba bean symbiosis

Cited by 28 publications

References 37 publications

Metabolomic Analysis of Cold Acclimation of Arctic Mesorhizobium sp. Strain N33

Metabolomic Analysis of Cold Acclimation of Arctic Mesorhizobium sp. Strain N33

Monounsaturated fatty acid aerobic synthesis inBradyrhizobiumTAL1000 peanut-nodulating is affected by temperature

Survival of Freeze-dried Leuconostoc mesenteroides and Lactobacillus plantarum Related to Their Cellular Fatty Acids Composition during Storage

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