This study aims to determine the digital literacy profile of Mathematics Education students at Universitas Negeri Makassar in online learning and its relationship with learning motivation. The research was designed with a quantitative approach ex-post facto model with the main method of survey. The research was conducted on Mathematics Education students at Universitas Negeri Makassar. The result of the studies: (1) mathematics education students have a high level of digital literacy, (2) mathematics education students have a high level of learning motivation, and (3) the relationship between digital literacy and learning motivation has a linear relationship and strong correlation between digital literacy and learning motivation.