In practical applications, problems related to proper curing arise for inclined structural elements, especially in skyscrapers, wherein concrete is very thick. To overcome this problem, the implementation of self-curing technology using varieties of smart materials has become significant. Based on these factors, this study determined the impact of effective microorganisms (EMs) as a new self-curing agent on the microstructures and strength properties of sustainable concrete. Five concrete mixtures were prepared with various EM content (5, 10, 15, 20, and 25%) as water replacement under air-curing condition. The workability of the concretes was found to increase with the increase in EM contents from 0 to 25%. In addition, concrete designed with 10% of EM achieved the highest compressive strength (42 MPa) after 28 days of aging as opposed to the control specimen (35 MPa). The microstructures of the concrete made with 10% of EM revealed very a compact network, fewer voids, and formulation of dense C-S-H gel. Based on the results, the proposed EM may be implemented as a self-curing agent to achieve high-performance sustainable concretes beneficial for the construction sectors.