The Effect of Electroacupuncture on PKMzeta in the ACC in Regulating Anxiety-Like Behaviors in Rats Experiencing Chronic Inflammatory Pain

Du, Junying; Fang, Jiaqi; Chen, Wen; Shao, Xiaomei; Liang, Yi; Fang, Jianqiao

doi:10.1155/2017/3728752

Cited by 36 publications

(29 citation statements)

References 44 publications

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“…29 Previous studies have demonstrated that EA treatment significantly suppresses CFA-induced anxiety-like behaviors as assessed by the openfield test and elevated zero maze. 26 Additionally, EA has been reported to attenuate the anxiety-like behavior induced by L5 spinal nerve ligation assessed by the elevated plus maze. 30 These and our data suggest that EA can regulate the affective disorders caused by inflammatory pain.…”

Section: Discussionmentioning

confidence: 99%

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<p>Proteomics analysis of the amygdala in rats with CFA-induced pain aversion with electro-acupuncture stimulation</p>

Wu¹,

Jiang²,

Shao³

et al. 2019

JPR

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Background: Clinical patients suffering from pain usually exhibit aversion to pain-associated environments (pain aversion). Electro-acupuncture (EA) has been proven to be effective for the treatment of pain aversion in our previous studies. The amygdala could have substantial consequences on emotion and pain consolidation as well as general pain aversion behavior, however, the underlying mechanism remains unclear. Purpose: The current study was performed to investigate Isobaric tags for relative and absolute quantitation (iTRAQ) based quantitative proteomic analysis of the amygdala in rats with complete Freund's adjuvant (CFA)-induced pain aversion, and comprehensive analysis of protein expression were performed to explore the underlying mechanism by which EA affects pain aversion. Materials and methods: Inflammatory pain was induced with an intraplantar injection of 100 μL of CFA in the plantar surface of the left hind paw of the male Spragure-Dawley (SD) rats. Then the CFA-induced conditioned place aversion (C-CPA) test was performed. EA stimulation on the bilateral Zusanli and Sanyinjiao acu-points was used for 14 days and the EA stimulation frequency is 2 Hz. Based on iTRAQ-based proteomics analysis, we investigated the protein expression in the amygdala. Results: EA can increase the paw withdrawal threshold in inflammatory pain induced by noxious stimulation. A total of 6319 proteins were quantified in amygdala. Of these identified proteins, 123 were identified in the pain aversion group relative to those in the saline group, and 125 significantly altered proteins were identified in the pain aversion + EA group relative to the pain aversion group. A total of 11 proteins were found to be differentially expressed in the amygdala of pain aversion and EA-treated rats. The expression of three proteins, glyceraldehyde-3-phosphate dehydrogenase, glutamate transporter-1, and p21-activated kinase 6, were confirmed to be consistent with the results of the proteome. Conclusion: Our investigation demonstrated the possible mechanism of central nerve system by which EA intervetion on pain aversion.

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Section: Discussionmentioning

confidence: 99%

“…Previous studies by our laboratory and others have reported that EA can significantly reduce the anxiety-like behaviors associated with chronic pain. 26 However, the mechanism for these effects is unclear. We speculate that the effect of EA on pain aversion might be associated with the amygdala, although this has not been fully explored.…”

Section: Introductionmentioning

confidence: 99%

<p>Proteomics analysis of the amygdala in rats with CFA-induced pain aversion with electro-acupuncture stimulation</p>

Wu¹,

Jiang²,

Shao³

et al. 2019

JPR

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“…Electroacupuncture (EA), whose efficacy has been widely recognized, is applied to treat different states of multiple acute and chronic pain diseases. Notably, it has been further validated that EA inhibited the affective dimension of pain on the basis of analgesia [37][38][39] Accordingly, proofs of the alleviation of pain and accompanied negative emotion performed by EA through the abovementioned brain areas bring us a new insight into the underlying mechanism [36,[40][41][42][43]. However, based on the limited number of studies, it remains to be adequately explored how EA promotes the relief of affective pain-related aspects by acting upon ACC, hypothalamus, and CeA.…”

Section: Introductionmentioning

confidence: 99%

Electroacupuncture Alleviates Pain‐Related Emotion by Upregulating the Expression of NPS and Its Receptor NPSR in the Anterior Cingulate Cortex and Hypothalamus

Fang

Xiang

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Correspondence should be addressed to Jianqiao Fang; fangjianqiao7532@163.com and Junying Du;Objective. Electroacupuncture (EA) is reported effective in alleviating pain-related emotion; however, the underlying mechanism of its effects still needs to be elucidated. e NPS-NPSR system has been validated for the involvement in the modulation of analgesia and emotional behavior. Here, we aimed to investigate the role of the NPS-NPSR system in the anterior cingulate cortex (ACC), hypothalamus, and central amygdala (CeA) in the use of EA to relieve affective pain modeled by complete Freund's adjuvant-(CFA-) evoked conditioned place aversion (C-CPA). Materials and Methods. CFA injection combined with a CPA paradigm was introduced to establish the C-CPA model, and the elevated O-maze (EOM) was used to test the behavioral changes after model establishment. We further explored the expression of NPS and NPSR at the protein and gene levels in the brain regions of interest by immunofluorescence staining and quantitative real-time PCR. Results. We observed that EA stimulation delivered to the bilateral Zusanli (ST36) and Kunlun (BL60) acupoints remarkably inhibited sensory pain, pain-evoked place aversion, and anxiety-like behavior. e current study showed that EA significantly enhanced the protein expression of this peptide system in the ACC and hypothalamus, while the elevated expression of NPSR protein alone was just confined to the affected side in the CeA. Moreover, EA remarkably upregulated the mRNA expression of NPS in CeA, ACC, and hypothalamus and NPSR mRNA in the hypothalamus and CeA. Conclusions. ese data suggest the effectiveness of EA in alleviating affective pain, and these benefits may at least partially be attributable to the upregulation of the NPS-NPSR system in the ACC and hypothalamus.

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“…Furthermore, Shao et al found that strong manual acupuncture stimulation could reduce pain-induced anxiety and phosphorylation of extracellular signal-regulated kinase in the anterior cingulate cortex (ACC) 29. Du et al confirmed that electro-acupuncture could decrease pain-induced anxiety-like behaviours by suppressing protein kinase mzeta activity in the ACC 30. Meanwhile, it has been shown that there is a shared neurobiological mechanism for emotional disorders and migraine 31–33.…”

Section: Introductionmentioning

confidence: 99%

Acupuncture for emotional disorders in patients with migraine: a systematic review protocol

Sun

et al. 2020

BMJ Open

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IntroductionMigraine is the second-leading cause of years lived with disability worldwide. The high prevalence of migraine-related emotional disorders is often overlooked. Acupuncture is often used to treat both migraine and emotional disorders. This systematic review protocol aims to analyse whether acupuncture is effective for treating emotional disorders in patients with migraine.Methods and analysisNine databases will be searched from inception to may 2019: cochrane central register of controlled trials, medline, embase, allied and complementary medicine database, cinahl, china national knowledge infrastructure, chinese biomedical literature database, vip database and wanfang database. Randomised controlled trials (rcts) of acupuncture therapy for migraine with emotional functioning outcomes, which were reported in chinese or english, will be included. The primary outcome is the change in emotional functioning. Study selection, data extraction and assessment of the risk of bias will be performed independently by two or more reviewers. Revman software (v.5.3) will be used to perform the assessment of the risk of bias and data synthesis.Ethics and disseminationEthics approval is not be needed because the data will not contain individual patient data, and there are no concerns about privacy. The results of this meta-analysis will be disseminated through publication in a peer-reviewed journal or relevant conference.Trial registration numberCRD42019139433.

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The Effect of Electroacupuncture on PKMzeta in the ACC in Regulating Anxiety-Like Behaviors in Rats Experiencing Chronic Inflammatory Pain

Cited by 36 publications

References 44 publications

<p>Proteomics analysis of the amygdala in rats with CFA-induced pain aversion with electro-acupuncture stimulation</p>

<p>Proteomics analysis of the amygdala in rats with CFA-induced pain aversion with electro-acupuncture stimulation</p>

Electroacupuncture Alleviates Pain‐Related Emotion by Upregulating the Expression of NPS and Its Receptor NPSR in the Anterior Cingulate Cortex and Hypothalamus

Acupuncture for emotional disorders in patients with migraine: a systematic review protocol

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