The effect of elicitors on oleanolic acid accumulation and expression of triterpenoid synthesis genes in Gentiana straminea

Zhao, Zhongjuan; Song, Yugang; Liu, Y. L.; Qiao, Meng; Zhai, Xiao-jie; Xiang, Fengning

doi:10.1007/s10535-012-0260-6

Cited by 24 publications

(7 citation statements)

References 23 publications

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“…The results were similar to the previous report, in Panax ginseng (C. A. Meyer) suspension cell, SQS , SE and β‐ AS expression levels were effectively enhanced with elicitor treatment . Moreover, in Gentiana straminea calli culture, elicitor significantly upregulated the genes including HMGR , FPS , GPS , SE and β‐ AS in the triterpenoid pathway . According to the report, the transcription level of β‐ AS gene was promoted in the treatment group; at the same time, triterpene saponins were enhanced .…”

Section: Resultssupporting

confidence: 89%

Gene expression of glycyrrhizin acid and accumulation of endogenous signaling molecule in Glycyrrhiza uralensis Fisch adventitious roots after Saccharomyces cerevisiae and Meyerozyma guilliermondii applications

Liu

Wang

et al. 2017

Biotech and App Biochem

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This study reports the best culture conditions for roots growth and accumulation of active components by optimizing the parameters. Glycyrrhiza uralensis adventitious roots metabolites were significantly increased after adding Saccharomyces cerevisiae and Meyerozyma guilliermondii. The highest contents of polysaccharide, glycyrrhizic acid, glycyrrhetinic acid, and total flavonoids were obtained in M. guilliermondii group; the content of glycyrrhizic acid was 5.3-fold higher than the control. In control and treatment groups, 12 compounds were identified by high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (HPLC-ESI-MS/MS), among which some new compounds have been detected in elicitor groups including 5,7-dihydroxyflavanone, glycyrrhisoflavanone, licorice saponin J2, uralsaponin B, (3R)-vestitol, and uralenol. Meyerozyma guilliermondii significantly upregulated the expression of the genes such as 3-hydroxy-3-methylglutaryl coenzyme A reductase, farnesyl diphosphate synthase, geranyl diphosphate synthase, squalene synthase, squalene epoxidase, β-amyrin synthase, and CYP88D6 and CYP72A154. Meanwhile, it increased the biosynthesis of signaling molecules (nitric oxide, salicylic acid, and jasmonic acid) in defense mechanism.

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Section: Resultssupporting

confidence: 89%

Gene expression of glycyrrhizin acid and accumulation of endogenous signaling molecule in Glycyrrhiza uralensis Fisch adventitious roots after Saccharomyces cerevisiae and Meyerozyma guilliermondii applications

Liu

Wang

et al. 2017

Biotech and App Biochem

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“…To date, the contribution of these genes to terpenoid (especially oleanolic acid) synthesis has not been well-elucidated in a medicinal plant species. We show here that the over-expression of GsAS2 enhanced the accumulation of oleanolic acid, a finding consistent with our previous results showing that oleanolic acid accumulation is positively related to the expression level of GsASs 46 . The relevant transgenic G. straminea plants could therefore make a significant contribution towards the engineering of this species to produce economically-viable amounts of this valuable metabolite.…”

Section: Discussionsupporting

confidence: 93%

An Intronless β-amyrin Synthase Gene is More Efficient in Oleanolic Acid Accumulation than its Paralog in Gentiana straminea

Liu

Zhao

Wang

et al. 2016

Sci Rep

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Paralogous members of the oxidosqualene cyclase (OSC) family encode a diversity of enzymes that are important in triterpenoid biosynthesis. This report describes the isolation of the Gentiana straminea gene GsAS2 that encodes a β-amyrin synthase (βAS) enzyme. Unlike its previously isolated paralog GsAS1, GsAS2 lacks introns. Its predicted protein product was is a 759 residue polypeptide that shares high homology with other known β-amyrin synthases (βASs). Heterologously expressed GsAS2 generates more β-amyrin in yeast than does GsAS1. Constitutive over-expression of GsAS2 resulted in a 5.7 fold increase in oleanolic acid accumulation, while over-expression of GsAS1 led to a 3 fold increase. Additionally, RNAi-directed suppression of GsAS2 and GsAS1 in G. straminea decreased oleonolic acid levels by 65.9% and 21% respectively, indicating that GsAS2 plays a more important role than GsAS1 in oleanolic acid biosynthesis in G. straminea. We uses a docking model to explore the catalytic mechanism of GsAS1/2 and predicted that GsAS2, with its Y560, have higher efficiency than GsAS1 and mutated versions of GsAS2 in β-amyrin produce. When the key residue in GsAS2 was mutagenized, it produced about 41.29% and 71.15% less β-amyrin than native, while the key residue in GsAS1 was mutagenized to that in GsAS2, the mutant produced 38.02% more β-amyrin than native GsAS1.

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“…In addition, Kylin et al considered that the increase of terpene content in pine needles during summers with elevated temperature may counteract the volatilization [28]. In contrast, low temperatures can negatively impact the accumulation of triterpenoids [19]. In Northeast China, air temperature tends to elevate sharply beginning in early May, and temperature usually begins to decline in late August.…”

Section: Discussionmentioning

confidence: 99%

Climatic Factors Shape the Spatial Distribution of Concentrations of Triterpenoids in Barks of White Birch (Betula Platyphylla Suk.) Trees in Northeast China

Guo

Zou

Wei

et al. 2017

Forests

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Betulin, betulinic acid and lupeol are naturally occurring pentacyclic triterpenoids with significant medicinal values. Great amounts of triterpenoids are found in the bark of white birch (Betula platyphylla Suk.) trees, which can be affected by climatic factors along the geographical gradients. In this study, site-based data of triterpenoids' (betulin, betulinic acid and lupeol) concentrations were determined in barks of white birch trees from 48 sites in Northeast China. Triterpenoid concentration in white birch tree barks did not change in response to any geographical gradients along latitudes, longitudes or elevations. Instead, concentrations of betulin and lupeol in birch tree barks increased with the increase of temperature and precipitation but declined with the increase of relative humidity. As a result, betulin concentration was higher in birch trees in the northeastern and southwestern parts of the study area, and lower in the central part of the study area in Northeast China. Although betulinic acid concentration did not change with climatic factors, its distribution pattern was similar to betulin concentration. Lupeol concentration was highest in the north-eastern part and along the southern and eastern boundaries in the study area. Our results can supply information for precondition of triterpenoids' extraction for industrial production, which can be an available approach to solve the issue of bark waste processing of white birch.

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The effect of elicitors on oleanolic acid accumulation and expression of triterpenoid synthesis genes in Gentiana straminea

Cited by 24 publications

References 23 publications

Gene expression of glycyrrhizin acid and accumulation of endogenous signaling molecule in Glycyrrhiza uralensis Fisch adventitious roots after Saccharomyces cerevisiae and Meyerozyma guilliermondii applications

Gene expression of glycyrrhizin acid and accumulation of endogenous signaling molecule in Glycyrrhiza uralensis Fisch adventitious roots after Saccharomyces cerevisiae and Meyerozyma guilliermondii applications

An Intronless β-amyrin Synthase Gene is More Efficient in Oleanolic Acid Accumulation than its Paralog in Gentiana straminea

Climatic Factors Shape the Spatial Distribution of Concentrations of Triterpenoids in Barks of White Birch (Betula Platyphylla Suk.) Trees in Northeast China

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