The effect of enamel matrix proteins on the spreading, proliferation and differentiation of osteoblasts cultured on titanium surfaces

Effect of enamel matrix derivative on the angiogenic behaviors of human umbilical vein endothelial cells on different titanium surfaces

“…Some previous studies showed that EMD stimulates differentiation of osteoblasts grown on moderately rough Ti surfaces 17,19) . One of these studies show that EMD promotes the proliferation and differentiation of osteoblasts an both PT and SLA surfaces, which suggest that the effect of EMD on osteoblasts does not depend on the Ti surface topography 29) . In contrast to this finding, we have observed that surface roughness is an important factor influencing the response of endothelial cells to EMD.…”

Effect of hyaluronic acid on morphological changes to dentin surfaces and subsequent effect on periodontal ligament cell survival, attachment, and spreading

“…Cells were quantified using fluorescent imaging (from live/dead assay) at 8 h for cell numbers as previously described [26]. At desired time point of 8 h, cells were washed with phosphate-buffered solution (PBS), fixed with 4 % formaldehyde solution (GroggChemie AG, Stettlen, Switzerland) for 5 min, and mounted with VECTASHILD (Vector, Burlingame, CA).…”

Implantatoberflächen und ihr Einfluss auf das periimplantäre Hartgewebe

“…Schmelzmatrixderivate ("enamel matrix derivatives", EMD), eine Mischung aus Matrixproteinen, die aus Zähnen gewonnen werden, wurden ebenfalls zur Beschichtung von Titanimplantaten verwendet. Die Beschichtung steigerte die Zellproliferation und erhöhte die Expression von Osteoblastenmarkern [76].…”