The effect of epigallocatechin-3-gallate (EGCG) on human alveolar bone cells both in vitro and in vivo

Mah, Yon Joo; Song, Je Seon; Kim, Seong‐Oh; Lee, Jae Ho; Jeon, Mijeong; Jung, Ui Won; Moon, Seok Jun; Kim, Jeong Hee; Choi, Hyung Jun

doi:10.1016/j.archoralbio.2014.02.011

Cited by 41 publications

(22 citation statements)

References 48 publications

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“…Human DPCs were then seeded at 2×10 4 cells/well onto the discs in 48-well plates. The ALP activity assay was performed as described elsewhere 17) . In brief, the level of ALP activity was measured using a SensoLyte � pNPP Alkaline Phosphatase Assay Kit (AnaSpec, Fremont, CA, USA), according to manufacturer' s instructions.…”

Section: Cell Viability Testmentioning

confidence: 99%

Biologic Response of Human Deciduous Dental Pulp Cells on Newly Developed MTA-like Materials

Lee¹,

Shin²,

Jung³

et al. 2015

THE JOURNAL OF THE KOREAN ACADEMY OF PEDTATRIC DENTISTRY

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This study compared the in vitro cell viability and differentiation potentials of human deciduous dental pulp cells (DPCs) on mineral trioxide aggregate (MTA)-like products (ProRoot MTA, RetroMTA and Endocem Zr). The experimental materials were prepared as circular discs, which were used to test the effects of the materials on the viability of human DPCs when placed in direct and indirect contact. Furthermore, the pH of the extracted materials was recorded, and their effect on cell differentiation potential was evaluated by evaluating the alkaline phosphatase (ALP) activity and Alizarin Red S staining of DPCs incubated with the test materials. In direct contact, the cell viability of human DPCs was higher with ProRoot MTA and RetroMTA than with Endocem Zr. However, when in indirect contact, the cell viability of human DPCs was generally higher in Endocem Zr than in ProRoot MTA and Retro MTA. With respect to pH, the alkalinity was lower for Endocem Zr than for the other test materials. The ALP activities of the cells were not enhanced by any of the experimental materials. Alizarin Red S staining of the tested human DPCs revealed that their differentiation potential was lower than for cells incubated with osteogenic induction medium. While there were differences in the responses of the human DPCs to the test materials, all displayed degrees of cytotoxicity and were unable to enhance either the viability or differentiation of human DPCs. However, Endocem Zr exhibited better cell viability and was less alkaline than the other test materials.

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Section: Cell Viability Testmentioning

confidence: 99%

Biologic Response of Human Deciduous Dental Pulp Cells on Newly Developed MTA-like Materials

Lee¹,

Shin²,

Jung³

et al. 2015

THE JOURNAL OF THE KOREAN ACADEMY OF PEDTATRIC DENTISTRY

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“…Green tea polyphenols including EGCG reportedly protected bone loss in middle-aged female rats following ovariectomy in vivo (31). It has also been reported that lower concentrations (1, 5 and 10 µM) of EGCG did not affect the migration of alveolar bone cells, whereas higher concentrations (25 and 50 µM) suppressed cell migration analyzed by wound-healing assay (32). In the present study, the significant suppressive effect of EGCG on the IGF-I-induced migration of MC3T3-E1 cells was observed at 1 µM in Transwell and wound healing assays.…”

Section: Discussionmentioning

confidence: 83%

Repression of IGF-I-induced osteoblast migration by (-)-epigallocatechin gallate through p44/p42 MAP kinase signaling

et al. 2018

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Polyphenolic compounds in beverages may have benefits in the prevention of osteoporosis. It has been demonstrated previously that insulin-like growth factor-I (IGF-I) could stimulate the migration of osteoblasts. In the present study, it was investigated whether chlorogenic acid, a major polyphenol in coffee, and (-)-epigallocatechin gallate (EGCG), a major polyphenol in green tea, could affect this IGF-I-stimulated migration of osteoblast-like MC3T3-E1 cells. The IGF-I-stimulated osteoblast migration, evaluated by Transwell cell migration and wound-healing assays, was inhibited by EGCG but not chlorogenic acid. IGF-I induced the phosphorylation of p44/p42 mitogen-activated protein (MAP) kinase, p70 S6 kinase and Akt. The IGF-I-induced migration was suppressed by PD98059, a MAP kinase kinase 1/2 inhibitor, and deguelin, an Akt inhibitor, but not rapamycin, an inhibitor of the upstream kinase of p70 S6 kinase (mammalian target of rapamycin). EGCG attenuated the IGF-I-induced phosphorylation of p44/p42 MAP kinase but not Akt. Taken together, the present results suggest that EGCG inhibits IGF-I-induced osteoblast migration via p44/p42 MAP kinase.

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“…Regarding non-malignant cells, it has been indicated that EGCG suppresses endothelial cell migration ( 32 ). In addition, in alveolar bone cells, lower concentrations of EGCG (1, 5 and 10 µ M) alone reportedly have no effect on the migration, whereas higher concentrations of EGCG (25 and 50 µ M) alone decrease the cell migration as revealed by a wound-healing assay ( 33 ). In the present study, a Transwell cell migration assay demonstrated that a lower concentration of EGCG, which had little effect on the cell migration in the absence of EGF, significantly reduced the EGF-induced migration of osteoblast-like MC3T3-E1 cells.…”

Section: Discussionmentioning

confidence: 99%

(-)-Epigallocatechin gallate but not chlorogenic acid suppresses EGF-stimulated migration of osteoblasts via attenuation of p38 MAPK activity

et al. 2018

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Phenolic compounds provide health benefits in humans. A previous study by our group has indicated that the epidermal growth factor (EGF)-induced migration of osteoblast-like MC3T3-E1 cells is mediated by the phosphorylation of p44/p42 mitogen-activated protein (MAPK), p38 MAPK, stress-activated protein kinase (SAPK)/c-Jun N-terminal kinase (JNK) and Akt, and that resveratrol, a major polyphenol in grape skin, suppresses the EGF-induced migration by attenuating Akt and SAPK/JNK activation. In the present study, the effects of chlorogenic acid, a major phenolic acid in coffee, and (−)-epigallocatechin gallate (EGCG), a major flavonoid in green tea, on the EGF-induced migration of MC3T3-E1 cells were investigated. EGCG significantly reduced the EGF-induced migration as evaluated by a Transwell migration assay and by a wound healing assay. However, chlorogenic acid failed to affect the EGF-induced migration. The phosphorylation of p38 MAPK induced by EGF was significantly suppressed by EGCG; however, the EGF-induced phosphorylation of p44/p42 MAP kinase, SAPK/JNK or Akt was not affected by EGCG. These results suggest that EGCG, but not chlorogenic acid, suppresses EGF-induced osteoblast migration through inhibiting p38 MAPK activation.

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The effect of epigallocatechin-3-gallate (EGCG) on human alveolar bone cells both in vitro and in vivo

Cited by 41 publications

References 48 publications

Biologic Response of Human Deciduous Dental Pulp Cells on Newly Developed MTA-like Materials

Biologic Response of Human Deciduous Dental Pulp Cells on Newly Developed MTA-like Materials

Repression of IGF-I-induced osteoblast migration by (-)-epigallocatechin gallate through p44/p42 MAP kinase signaling

(-)-Epigallocatechin gallate but not chlorogenic acid suppresses EGF-stimulated migration of osteoblasts via attenuation of p38 MAPK activity

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