The effect of erythropoietin and umbilical cord-derived mesenchymal stem cells on nerve regeneration in rats with sciatic nerve injury

Ülger, Menekşe; Sezer, Gülay; Özyazgan, İrfan; Özocak, Hakan; Yay, Arzu; Balcıoğlu, Esra; Yalçın, Betül; Göç, Rümeysa; Ülger, Birkan; Özyazgan, Tuğçe Merve; Yakan, Birkan

doi:10.1016/j.jchemneu.2021.101958

Cited by 9 publications

(6 citation statements)

References 55 publications

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“…To date, given the multi-dimensional and complex nature of nerve injury and regeneration, combining pharmacological therapies has gained researchers' attention willing to recruit their possible synergism on peripheral nerve regeneration (18,31,38). For instance, Labroo et al uncovered the potentiating effects of tacrolimus, nerve growth factor (NGF), and glial cell-derived neurotrophic factor on neurite elongation compared to their corresponding individual doses (31).…”

Section: Discussionmentioning

confidence: 99%

The Effects of Tacrolimus and Erythropoietin on Histopathologic and Functional Recovery of Sciatic Nerve Crush in Mice

Mansouri

Fattahian

Jahandideh

et al. 2023

Macedonian Veterinary Review

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Currently, despite decades of trial and error, peripheral nerve injury is an impenetrable clinical dilemma. Any proven effective pharmacologic agent leads to a decisive leap forward to the clinical management of neuropathies. This study investigated the effects of tacrolimus and erythropoietin on sciatic nerve regeneration. Twenty-three mice were randomly assigned to tacrolimus, erythropoietin, tacrolimus + erythropoietin, control, and sham groups following sciatic nerve crush via hemostatic forceps. Medications were administered for 28 consecutive days. The sham group received neither crush injury nor medication. Histopathologic, immunohistochemical, and walking track analyses were performed. In the erythropoietin group, axonal swelling was significantly reduced and the average axonal number significantly recovered up to 75% of normal nerve compared to other groups. Marked immunoreactivity to GFAP and S-100 protein was present in the tacrolimus group. Nevertheless, at least moderate GFAP and S-100 expressions were observed in all of the groups. Functional recovery was superior in the tacrolimus group after 14 days, although a complete return to near-normal function was achieved in all groups after 28 days, regardless of the medication used. Our data supported the neurotrophic effects of tacrolimus and erythropoietin; however, not enough data was gathered to confirm their synergistic effects. Whether these results are extensible to clinical scenarios requires further detailed investigations.

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Section: Discussionmentioning

confidence: 99%

The Effects of Tacrolimus and Erythropoietin on Histopathologic and Functional Recovery of Sciatic Nerve Crush in Mice

Mansouri

Fattahian

Jahandideh

et al. 2023

Macedonian Veterinary Review

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“…In the study, UC-MSCs, which were isolated from the umbilical cord of volunteers and characterized by immunofluorescence staining and flow cytometry, as specified in the study of Ülger et al,[ 15 ] were used with the approval of the Erciyes University Clinical Research Ethics Committee dated April 4, 2018, and numbered 2018/182. In the second passage, UC-MSCs that highly express positive markers that should be found in MSCs in flow cytometry but do not express negative markers show adipogenic and osteogenic differentiation by immunofluorescence staining were kept in a nitrogen tank at −196°C to be used in the study.…”

Section: Methodsmentioning

confidence: 99%

Investigation of the effects of umbilical cord-derived mesenchymal stem cells and curcumin on Achilles tendon healing – can they act synergistically?

Öztürk

2023

Ulus Travma Acil Cerrahi Derg

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BACKGROUND: It is known that curcumin and umbilical cord-derived mesenchymal stem cells (UC-MSCs) positively affect experimental tendon injury healing. This study investigated individual effects and potential synergistic effects of using curcumin and UC-MSCs alone and together. METHODS: Eighty female Wistar albino rats were randomly divided into five groups: Control, curcumin, sesame oil, MSCs, and Curcumin+MSCs groups. In all rats, punch tendon defect was created in both right and left Achilles tendons. While no additional treatment was applied to the control group, curcumin, sesame oil used as a solvent for curcumin, MSCs, and MSCs and curcumin combination were applied locally to the injury site, respectively, in the other groups. Curcumin was solved in sesame oil before application. In each group, half of the animals were euthanized in the post-operative 2nd week while the other half were euthanized in the post-operative 4th week. The right Achilles was used for biomechanical testing, while the left Achilles was used for histological evaluation and immunohistochemical analysis of type I, Type III collagen, and tenomodulin. RESULTS: Histologically, significant improvement was observed in the curcumin, MSCs, and Curcumin+ MSCs groups compared to the control Group in the 2nd week. In the 2nd and 4th weeks, Type III collagen was significantly increased in the curcumin group compared to the control group. In week 4, tenomodulin increased significantly in the curcumin and MSCs groups compared to the control group. Tendon tensile strength increased significantly in MSCs and Curcumin+MSCs groups compared to the control group in the 4th week. No superiority was observed between the treatment groups regarding their positive effects on recovery. CONCLUSION: Locally used curcumin and UC-MSCs showed positive effects that were not superior to each other in the healing of injury caused by a punch in the Achilles tendons of rats. However, synergistic effects on healing were not observed when they were applied together.

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“…hAM alone or associated with adult or perinatal cells ( Fénelon et al, 2021 ), such as allogeneic hUC-MSC ( Li et al, 2013 ), has been evaluated. In animal models, allogeneic hUC-MSC alone or coupled to additional scaffolds like PCL polycaprolactone (PCL) or chitosan were delivered at the site of injury ( Bojanic et al, 2020 ), supplemented with systemic erythropoietin injection ( Ülger et al, 2021 ).…”

Section: Specific Sections Dedicated To Pnd Applicationsmentioning

confidence: 99%

“…Histological (HE, Toluidine Blue, Oil Red O staining) or immunohistological analysis have allowed qualitative/stereological analysis of the gross morphology or microstructure of: 1) nerve parameters such as axon regeneration—increase of axon size—number—diameter—density, axonal migration, myelin sheath thickness or nerve fiber diameter; 2) muscle mass innervated by the transected then repaired nerve and 3) muscle atrophy attenuation ( Bour geois et al, 2019 ; Bojanic et al, 2020 ; Ülger et al, 2021 ; Wolfe et al, 2022 ). Retrograde axonal transport was estimated through fluorogold-labeled neuron counts after hUC-MSC grafting ( Bojanic et al, 2020 ).…”

Section: Specific Sections Dedicated To Pnd Applicationsmentioning

confidence: 99%

“…Functional motor assessments included various types of static or dynamic gait analysis, strength measurements, or analysis of other specific behavior ( Su et al, 2018 ; Bour geois et al, 2019 ; Ülger et al, 2021 ; Wolfe et al, 2022 ). The sciatic functional index (SFI) is an index of the functional condition of rat sciatic nerve based on measurements made from walking tracks ( Ülger et al, 2021 ). Functional sensory outcomes measures also included withdrawal latency reflex (WRL) tests, tactile tests, or heat cutaneous allodynia ( Wolfe et al, 2022 ).…”

Section: Specific Sections Dedicated To Pnd Applicationsmentioning

confidence: 99%

See 1 more Smart Citation

General consensus on multimodal functions and validation analysis of perinatal derivatives for regenerative medicine applications

Pozzobon

D’Agostino

Roubelakis

et al. 2022

Front. Bioeng. Biotechnol.

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Perinatal tissues, such as placenta and umbilical cord contain a variety of somatic stem cell types, spanning from the largely used hematopoietic stem and progenitor cells to the most recently described broadly multipotent epithelial and stromal cells. As perinatal derivatives (PnD), several of these cell types and related products provide an interesting regenerative potential for a variety of diseases. Within COST SPRINT Action, we continue our review series, revising and summarizing the modalities of action and proposed medical approaches using PnD products: cells, secretome, extracellular vesicles, and decellularized tissues. Focusing on the brain, bone, skeletal muscle, heart, intestinal, liver, and lung pathologies, we discuss the importance of potency testing in validating PnD therapeutics, and critically evaluate the concept of PnD application in the field of tissue regeneration. Hereby we aim to shed light on the actual therapeutic properties of PnD, with an open eye for future clinical application. This review is part of a quadrinomial series on functional/potency assays for validation of PnD, spanning biological functions, such as immunomodulation, anti-microbial/anti-cancer, anti-inflammation, wound healing, angiogenesis, and regeneration.

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The effect of erythropoietin and umbilical cord-derived mesenchymal stem cells on nerve regeneration in rats with sciatic nerve injury

Cited by 9 publications

References 55 publications

The Effects of Tacrolimus and Erythropoietin on Histopathologic and Functional Recovery of Sciatic Nerve Crush in Mice

The Effects of Tacrolimus and Erythropoietin on Histopathologic and Functional Recovery of Sciatic Nerve Crush in Mice

Investigation of the effects of umbilical cord-derived mesenchymal stem cells and curcumin on Achilles tendon healing – can they act synergistically?

General consensus on multimodal functions and validation analysis of perinatal derivatives for regenerative medicine applications

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