The effect of estradiol on the expression of estrogen, progesterone, androgen, and prolactin receptors in human peritoneal fibroblasts

Detti, Laura; Saed, Ghassan M.; Jiang, Zhong; Krüger, Michael; Diamond, Michael P.

doi:10.1007/s10815-008-9230-5

Cited by 9 publications

(8 citation statements)

References 18 publications

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“…Assuming the same number of patients in the 2 groups, we needed 8 patients in each group. The choice of 30% difference was arbitrary, but based on 2 assumptions: in normal endometria, PR-B decreases during the secretory phase (10); in a previous study, peritoneal fibroblasts were treated with estradiol to mimic ovarian stimulation during ART, and we found a 10% increase in PR-A+B (8). …”

Section: Methodsmentioning

confidence: 98%

“…To quantify each target transcript, a standard curve was constructed using a tenfold dilution series of β-actin standard plasmid (Invitrogen Corp, Carlsbaad, CA). We used β-actin as house-keeping gene in a previous study on peritoneal fibroblasts (8). However, other studies have reported on the stability of beta-actin in endometrial samples (9).…”

Section: Methodsmentioning

confidence: 99%

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Endometrial morphology and modulation of hormone receptors during ovarian stimulation for assisted reproductive technology cycles

Detti

Saed²,

Fletcher³

et al. 2011

Fertility and Sterility

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Objective To study the biochemical and morphologic implications of different hormonal levels on endometrial development during ART cycles. Design Prospective controlled study. Setting University center. Patients Eleven women during a natural (controls) and 11 oocyte donors during an ART cycle (treated). Interventions At the time consistent with day 3 embryo transfer, a transvaginal ultrasound, an endometrial biopsy, and blood sampling were performed. Morphology and thickness of the endometrial stripe were recorded. Real-time RT-PCR was used to measure mRNA levels for ER-α, ER-β, PR-A and PR-B in the endometrial tissue. Main Outcome Measures To evaluate morphologic and biochemical endometrial development. Results Endometrium was mostly trilaminar (proliferative-like pattern) and thicker in the treated group as opposed to homogeneous and thinner in the controls. PR-B mRNA expression increased 41% in treated patients (p<0.05); PR-A mRNA expression, instead, was unchanged. Serum estradiol and progesterone were higher in the treated group than in controls. FSH and LH levels were similar. Conclusions When compared with natural cycles, exposure of the endometrium to high hormone levels during ovarian stimulation significantly increased PR-B receptor expression at the time of embryo transfer. Concurrently, a proliferative-like endometrial pattern persisted. These findings reflect a delayed endometrial development in ART.

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Section: Methodsmentioning

confidence: 98%

Section: Methodsmentioning

confidence: 99%

Endometrial morphology and modulation of hormone receptors during ovarian stimulation for assisted reproductive technology cycles

Detti

Saed²,

Fletcher³

et al. 2011

Fertility and Sterility

Self Cite

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“…Jurkat cells (2 × 10 6 /mL) were then incubated in T25 flasks with 0, 4, or 40 pg/mL estradiol (E 2 ) for 48 hr. Based on recent studies, the 4 pg/mL estradiol level was used to correspond to the post‐menopausal levels (midpoint within the observed post‐menopausal range), 21 while 40 pg/mL estradiol represents a level associated with the mean of follicular and luteal phases of pre‐menopausal women 22 . For each set of estradiol conditions, cells were coincubated with 0.5 or 5 μ m of genistein, representing physiologically achievable levels with diets and/or supplements 23,24 .…”

Section: Methodsmentioning

confidence: 99%

ORIGINAL ARTICLE: Genistein Reverses Diminished T‐Cell Signal Transduction, Induced by Post‐Menopausal Estrogen Levels

Parry

Taylor

Nakajima

et al. 2008

American J Rep Immunol

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“…To quantify each target transcript, a standard curve was constructed with the use of a tenfold dilution series of β -actin standard plasmid (Invitrogen). We used β -actin as housekeeping gene in previous studies on peritoneal fibroblasts (2, 27). Other studies have reported on the stability of β -actin in endometrial samples (28).…”

Section: Methodsmentioning

confidence: 99%

Endometrial signaling pathways during ovarian stimulation for assisted reproduction technology

Detti

Uhlmann

Fletcher

et al. 2013

Fertility and Sterility

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Objective To determine the effects of different hormonal levels on endometrial biochemical development during ovulation induction for assisted reproduction technology (ART) cycles. Design Prospective controlled study. Setting University center. Patient(s) Nine women during a natural cycle (control) and 9 oocyte donors (treated) during an ART cycle. Intervention(s) At the time consistent with day 3 embryo transfer (LH+5 in control, hCG+5 in treated), transvaginal ultrasound, endometrial biopsy, and blood sampling were performed. Real-time reverse-transcription polymerase chain reaction was used to measure mRNA levels for insulin receptor (InsR), type I IGF receptor (IGFRI), prolactin receptor (PRL-R), androgen receptor (AR), TSH receptor (TSHR), nuclear receptors for T3 and T4 (TRα1, TRα2, and TRβ1), iodothyronine deiodinase (DIO2), and 1,25-dihydroxyvitamin D3 receptor (VDR) in the endometrial tissue. Main Outcome Measure(s) Biochemical endometrial development. Result(s) IGFRI mRNA levels were 69% lower in treated patients than in control subjects, 0.12 ± 0.005 pg/μg RNA versus 0.39 ± 0.01 pg/μg RNA. TSHR mRNA was 57% lower, 2.6 ± 0.1 fg/μg RNA versus 6.0 ± 0.2 fg/μg RNA. TRα1 and TRα2 mRNA did not change, but TRβ1 mRNA levels were 63% higher. DIO2 mRNA was 63% lower, 1.2 ± 0.07 pg/μg RNA versus 3.2 ± 0.2 pg/μg RNA. InsR mRNA levels, despite being 68% lower in treated patients, did not reach significance, and PRL-R, AR, and VDR did not significantly change. Conclusion(s) Exposure of the endometrium to ovarian stimulation appears to influence insulin and thyroid hormone signaling pathways in the decidua at day 3 embryo transfer, whereas prolactin, androgen, and vitamin D pathways are uninfluenced. These findings echo the known delayed endometrial maturation during ovarian stimulation.

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The effect of estradiol on the expression of estrogen, progesterone, androgen, and prolactin receptors in human peritoneal fibroblasts

Abstract: The inflammatory-like changes manifested by adhesion fibroblasts enhance the anabolic hormones receptor expression (ER-alpha, ER-beta, PRL, and A-R), when exposed to estradiol.

Cited by 9 publications

References 18 publications

Endometrial morphology and modulation of hormone receptors during ovarian stimulation for assisted reproductive technology cycles

Endometrial morphology and modulation of hormone receptors during ovarian stimulation for assisted reproductive technology cycles

ORIGINAL ARTICLE: Genistein Reverses Diminished T‐Cell Signal Transduction, Induced by Post‐Menopausal Estrogen Levels

Endometrial signaling pathways during ovarian stimulation for assisted reproduction technology

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