The effect of exercise training on oxidative damage of lipids, proteins, and DNA in rat skeletal muscle: evidence for beneficial outcomes

Radák, Zsolt; Kaneko, Takao; Tahara, Shoichi; Nakamoto, Hideko; Ohno, Hideki; Sasvári, Mária; Nyakas, Csaba; Goto, Sataro

doi:10.1016/s0891-5849(99)00038-6

Cited by 228 publications

(190 citation statements)

References 35 publications

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“…At 18 h, PCC level was markedly reduced to Ϸ50% of the value at early reperfusion (2 min to 4 h) (38). In another animal study it was indicated that muscle PCC level was unaffected by extensive exercise or age (39,40).…”

Section: Discussionmentioning

confidence: 97%

Plasma antioxidant status and cell injury after severe physical exercise

Chevion

Moran

Heled

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

221

137

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Strenuous exercise leads to an increase in metabolic rate, increased production of reactive oxygen species, and compromised antioxidant defense systems. To study the effects of oxidative stress during strenuous exercise, a homogeneous group of 31 male subjects participated in a 6-month, 5 days͞week training schedule involving two extreme marches of 50 km and 80 km at sea level, separated by 2 weeks of regular training. Each participant carried 35 kg of extra weight. Blood samples were drawn imediately before and after each march. Twenty-nine subjects completed the 50-km march, and only 16 completed the 80-km march. Plasma levels of reduced ascorbic acid, total ascorbate, and dehydroascorbate did not undergo significant changes during either march. However, the 50-and 80-km marches led to 25% and 37% increases, respectively, in plasma levels of uric acid; due presumably to increases in the metabolic rate and consequent pyrimidine nucleotide metabolism. Both marches led to Ϸ10-fold increase leakage of creatine phosphokinase into the plasma. Likewise, plasma levels of aspartate transaminase, a characteristic marker of liver injury, increased Ϸ4-fold. Plasma levels of bilirubin, creatine, urea, and glucose also increased. Plasma protein carbonyl content, a marker of protein oxidative damage, decreased significantly during each march. These results are discussed with respect to the consideration that elevation of the respiration rate during exercise leads to production of more reactive oxygen species than the antioxidant systems can scavenge. Plausible explanations for leakage of molecules into the plasma are discussed.

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Section: Discussionmentioning

confidence: 97%

Plasma antioxidant status and cell injury after severe physical exercise

Chevion

Moran

Heled

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

221

137

View full text Add to dashboard Cite

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“…In another study, Rádak and co-workers (12) have shown an important increase in the CS activity in different skeletal muscles in young and middle age rats after a nine week training in water. Although our study confirms these results, other studies mentioned by Pereira and co-workers (21) has presented opposite responses.…”

Section: Discussionmentioning

confidence: 87%

“…Several studies have described the metabolic ways in the ROS production while exercising (9)(10)(11)(12) , but little is yet known about the influence of the regular physical exercises on the ROS releasing after inhaling mineral particles. The information contained in the literature is only presuppositions that demand further scientific investigations.…”

Section: Introductionmentioning

confidence: 99%

Exercício físico regular diminui o estresse oxidativo pulmonar em ratos após exposição aguda ao carvão mineral

Pinho

Piazza

Tuon

et al. 2006

Rev Bras Med Esporte

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Several studies have pointed the regular low to moderate intensity physical exercise an important agent to combat the oxidant stress. The purpose of this study was to investigate the effect of the physical exercise in the pulmonary oxidant response after inhaling mineral coal dust. Twenty-four male Wistar rats (200-250 g) were randomly divided in two groups with their respective controls (trained, n = 6; non-trained, n = 6). All animals received mineral coal dust via tracheal instillation (saline 3 mg/0.5 ml, 3 days/ week for 3 weeks), or 0.5 ml of 0.9% saline solution. Forty-eight hours after the last instillation, the trained group was submitted to a progressive exercise program on treadmill for twelve weeks (up to 17 m.min , 10% inclination). Forty-eight hours after the last training session, every animal was killed by decapitation, and their lungs and soleus were surgically removed for later biochemical analysis. The citrate synthase activity was determined in the soleus muscle, and the damages on the lipids and proteins were assessed into the lungs by means of the TBARS concentration and by determining the carbonyl groups, respectively. The results show that the regular practice of physical exercises reduces significantly the present TBARS levels in trained rats, decreasing the oxidation levels in proteins of both groups compared to their respective controls. The results lead us to suggest that the regular physical exercise on treadmill is an agent capable to soften the pulmonary oxidant damages caused by inhaling mineral coal particles.

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“…Such fact may occur both by increase in the formation of reactive oxygen species (ROSs) and by decrease of capacity of cellular antioxidant defense. This damage includes increase of lipid peroxidation levels (oxidation of the lipid layer of the cell membrane), increase in the carbonylation of proteins and even damage in the intracellular DNA, which affects the intracellular metabolism and can cause apoptosis (cell death) (1)(2)(3)(4) . Physical exercise promotes increase in the energetic demand of up to 35 times compared to rest (5) .…”

Section: Introductionmentioning

confidence: 99%

“…Physical exercise promotes increase in the energetic demand of up to 35 times compared to rest (5) . Knowing that about 2% to 5% of the O 2 consumed gives origin to ROS in the mitochondria (2,6) , the increase in O 2 consumption induced by physical exercise is associated with the increase of the ROS being these inducers of oxidative stress. Dillard et al (7) were the pioneers in demonstrating that physical exercise induces increase of lipid peroxidation.…”

Section: Introductionmentioning

confidence: 99%

A fototerapia com diodo emissor de luz (LEDT) aplicada pré-exercício inibe a peroxidação lipídica em atletas após exercício de alta intensidade: um estudo preliminar

Leal

Baroni

Rossi

et al. 2011

Rev Bras Med Esporte

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Oxidative stress is the term generally used to describe the damage caused by imbalance between prooxidants and antioxidants in the organism. The increase in the O2 consumption induced by physical exercise is associated with the increase of reactive oxygen species (ROS) being these species inducers of oxidative stress. Although the evidence indicates a probable inhibitory effect of the light emitting diode therapy (LEDT) on the production of ROS, there are no studies observing this effect in humans. This preliminary study has the aim to verify the effects of LEDT applied before high-intensity exercise on lipid peroxidation, measured through blood levels of ThioBarbituric Acid Reactive Substances (TBARS). Six male volleyball athletes were submitted to two situations: active LEDT and placebo LEDT. Performance in the exercise protocol showed no difference (p> 0.05) between the two situations in peak power, average power and fatigue index. The results related to lipid peroxidation were: at active LEDT situation, it was not possible to observe statistically significant difference (p>0.05) between pre and post exercise levels (6.98 ± 0.81 and 7.02 ± 0.47 nmol/ mL); at placebo LEDT situation, statistically significant difference (p=0.05) was observed between pre and post exercise levels (7.09 ± 1.28 and 8.43 ± 0.71 nmol/mL). These results show that active LEDT seems to be effective in controlling lipid peroxidation in athletes submitted to intense exercise.

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The effect of exercise training on oxidative damage of lipids, proteins, and DNA in rat skeletal muscle: evidence for beneficial outcomes

Cited by 228 publications

References 35 publications

Plasma antioxidant status and cell injury after severe physical exercise

Plasma antioxidant status and cell injury after severe physical exercise

Exercício físico regular diminui o estresse oxidativo pulmonar em ratos após exposição aguda ao carvão mineral

A fototerapia com diodo emissor de luz (LEDT) aplicada pré-exercício inibe a peroxidação lipídica em atletas após exercício de alta intensidade: um estudo preliminar

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