The Effect of Exogenous Peroxiredoxin 6 on the State of Mesenteric Vessels and the Small Intestine in Ischemia–Reperfusion Injury

Шарапов, М. Г.; Gordeeva, A. E.; Гончаров, Р. Г.; Tikhonova, Irina V.; Равин, В. К.; Temnov, A. A.; Фесенко, Е. Е.; Новоселов, В. И.

doi:10.1134/s0006350917060239

Cited by 15 publications

(8 citation statements)

References 46 publications

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“…We found that injection of Prdx6 significantly protects the small intestine from the damaging effects of ionizing radiation (Figure 7). We must note that the protective effect of exogenous Prdx6 has also been shown earlier on an ischemia–reperfusion model of small intestine injury [106,107].…”

Section: Application Of Exogenous Prdx6 As a Radioprotectormentioning

confidence: 59%

“…Experiments in animal models of diseases accompanied by development of oxidative stress showed that application of exogenous Prdx6 can correct the pathologic process course and facilitate faster recovery of damaged organs and tissues [103,106,107,108]. Particularly, exogenous Prdx6 application alleviates the severity of ionizing radiation damage, increasing the survival rate of animals exposed to sublethal and lethal doses [110,111].…”

Section: Discussionmentioning

confidence: 99%

“…Application of exogenous recombinant Prdx6 in model animal experiments has shown high efficiency in the treatment of disorders attended by oxidative stress, such as severe chemical and thermal burns of the upper respiratory tract, acute inflammation of respiratory organs caused by bacterial endotoxins (LPS), and ischemia-reperfusion injuries [103,104,105,106,107]. Infusion of Prdx6 before the action of the aforementioned factors provides suppression of oxidative stress, thus preventing the damage of actively proliferating tissues.…”

Section: Application Of Exogenous Prdx6 As a Radioprotectormentioning

confidence: 99%

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Radioprotective Role of Peroxiredoxin 6

2019

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Peroxiredoxin 6 (Prdx6) is a member of an evolutionary ancient family of peroxidase enzymes with diverse functions in the cell. Prdx6 is an important enzymatic antioxidant. It reduces a wide range of peroxide substrates in the cell, thus playing a leading role in the maintenance of the redox homeostasis in mammalian cells. Beside peroxidase activity, Prdx6 has been shown to possess an activity of phospholipase A2, an enzyme playing an important role in membrane phospholipid metabolism. Moreover, Prdx6 takes part in intercellular and intracellular signal transduction due to its peroxidase and phospholipase activity, thus facilitating the initiation of regenerative processes in the cell, suppression of apoptosis, and activation of cell proliferation. Being an effective and important antioxidant enzyme, Prdx6 plays an essential role in neutralizing oxidative stress caused by various factors, including action of ionizing radiation. Endogenous Prdx6 has been shown to possess a significant radioprotective potential in cellular and animal models. Moreover, intravenous infusion of recombinant Prdx6 to animals before irradiation at lethal or sublethal doses has shown its high radioprotective effect. Exogenous Prdx6 effectively alleviates the severeness of radiation lesions, providing normalization of the functional state of radiosensitive organs and tissues, and leads to a significant elevation of the survival rate of animals. Prdx6 can be considered as a potent and promising radioprotective agent for reducing the pathological effect of ionizing radiation on mammalian organisms. The radioprotective properties and mechanisms of radioprotective action of Prdx6 are discussed in the current review.

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Section: Application Of Exogenous Prdx6 As a Radioprotectormentioning

confidence: 59%

Section: Discussionmentioning

confidence: 99%

Section: Application Of Exogenous Prdx6 As a Radioprotectormentioning

confidence: 99%

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Radioprotective Role of Peroxiredoxin 6

2019

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“…As discussed earlier, the decrease in the activation of iNOS and eNOS may be due to the suppression of the NF-kB expression, as well as to the normalization of the microvasculature under the action of exogenous Prx1 and Prx2 [ 82 ]. Moreover, it has been shown earlier that exogenous Prx6 administration contributes to the rapid restoration of the blood flow in the microvasculature upon I/R of the small intestine and mesenteric vessels [ 13 , 83 ].…”

Section: Discussionmentioning

confidence: 99%

Comparative Study of Protective Action of Exogenous 2-Cys Peroxiredoxins (Prx1 and Prx2) Under Renal Ischemia-Reperfusion Injury

et al. 2020

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The pathogenesis of ischemia-reperfusion (I/R) injuries is based on oxidative stress caused by a sharp increase in the concentration of free radicals, reactive oxygen species (ROS) and secondary products of free radical oxidation of biological macromolecules during reperfusion. Application of exogenous antioxidants lowers the level of ROS in the affected tissues, suppresses or adjusts the course of oxidative stress, thereby substantially reducing the severity of I/R injury. We believe that the use of antioxidant enzymes may be the most promising line of effort since they possess higher efficiency than low molecular weight antioxidants. Among antioxidant enzymes, of great interest are peroxiredoxins (Prx1–6) which reduce a wide range of organic and inorganic peroxide substrates. In an animal model of bilateral I/R injury of kidneys (using histological, biochemical, and molecular biological methods) it was shown that intravenous administration of recombinant typical 2-Cys peroxiredoxins (Prx1 and Prx2) effectively reduces the severity of I/R damage, contributing to the normalization of the structural and functional state of the kidneys and an almost 2-fold increase in the survival of experimental animals. The use of recombinant Prx1 or Prx2 can be an efficient approach for the prevention and treatment of renal I/R injury.

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“…В этом случае для снижения концентрации активных форм кислорода и уменьшения поражения изолированных органов основным направлением терапии может стать использование антиоксидантных препаратов, в част ности, использование антиоксидантных ферментов семейства пероксиредоксинов, в связи с их широким распространением в организме и мультифункциональностью [10][11][12][13]. Среди представителей семейства пероксиредоксину 6 (Prx6) посвящено наибольшее количество работ, указывающих на его протекторную роль при свободно-радикальных патологиях [8,[14][15][16], в том числе и в защите изолированных органов от И-РП [17,18]. Учитывая протекторную роль Prx6, следует изучить возможность его применения для сохранения морфофункционального состояния изолированной ишемизированной почки.…”

Section: Introductionunclassified

The effect of exogenous peroxiredoxin 6 on morphofunctional state of isolated rat kidney

Gordeeva

Шарапов

Новоселов

2021

RJTAO

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Objective: to investigate the role of peroxiredoxin 6 (PRX6) in preserving the morphofunctional state of ischemic isolated kidney during perfusion.Materials and methods. The model of an isolated perfused rat kidney was used. Ischemia time was 5 and 20 minutes, perfusion was 50 minutes. To evaluate the effectiveness of PRX6 at different ischemia times, we used the conventional criteria of kidney function and histological methods.Results. During short warm ischemia times, exogenous PRX6 improves the morphofunctional state of an isolated kidney during perfusion. During this period, the main criteria for functioning of the isolated ischemic kidney reach acceptable values, renal parenchyma is without severe damage. By the end of perfusion, there was an increase in urine flow rate, glomerular filtration rate, fractional glucose reabsorption, urine urea concentration and proportion of primary urine from 1.5 to 2 times compared with the control lesion. At 20-minute ischemia, the isolated kidney can be recognized as non-viable according to the functioning criteria; the positive effect of PRX6 is leveled.Conclusion. The use of recombinant peroxiredoxin 6 for preserving the morphofunctional state of isolated kidneys can be an effective approach in preventing ischemia–reperfusion injury.

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The Effect of Exogenous Peroxiredoxin 6 on the State of Mesenteric Vessels and the Small Intestine in Ischemia–Reperfusion Injury

Cited by 15 publications

References 46 publications

Radioprotective Role of Peroxiredoxin 6

Radioprotective Role of Peroxiredoxin 6

Comparative Study of Protective Action of Exogenous 2-Cys Peroxiredoxins (Prx1 and Prx2) Under Renal Ischemia-Reperfusion Injury

The effect of exogenous peroxiredoxin 6 on morphofunctional state of isolated rat kidney

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