The effect of experimental diabetes upon the breaking strength of the healing fracture in the rat

Wray, James B.; Stunkle, Eugene

doi:10.1016/s0022-4804(65)80046-4

Cited by 21 publications

(19 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…[2][3][4][5] In animal models, diabetes has been reported to cause a reduction in early cellular proliferation, a delay in chondrogenesis, and a decrease in the biomechanical properties of the fracture callus. 7 The precise molecular mechanism by which diabetes impairs these fracture healing processes is unknown.…”

Section: Discussionmentioning

confidence: 99%

“…1 The association between diabetes and impaired osseous healing has been documented in clinical and experimental settings. [2][3][4][5] Several clinical studies have evaluated complications following elective arthrodesis in diabetes patients and noted a significant incidence of delayed union, nonunion, and pseudoarthrosis. 4 Furthermore, chemically induced and spontaneous diabetic animal models also have demonstrated impairments in fracture healing.…”

mentioning

confidence: 99%

“…4 Furthermore, chemically induced and spontaneous diabetic animal models also have demonstrated impairments in fracture healing. [5][6][7][8] In various fracture healing models, diabetes correlated with a reduction in fracture callus cellular proliferation, collagen synthesis, and biomechanical properties. 2,7,9,10 The mechanism by which diabetes impairs fracture healing is unknown.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Recombinant human platelet‐derived growth factor BB (rhPDGF‐BB) and beta‐tricalcium phosphate/collagen matrix enhance fracture healing in a diabetic rat model

Al-Zube

Breitbart

O’Connor

et al. 2009

Journal Orthopaedic Research

View full text Add to dashboard Cite

Diabetes mellitus is a common systemic disease that has been associated with poor fracture healing outcomes. The mechanism through which diabetes impairs bone regeneration is unknown. One possible mechanism may be related to either decreased or uncoordinated release of local growth factors at the fracture site. Indeed, previous studies have found reduced platelet-derived growth factor (PDGF) levels in the fracture callus of diabetic rats, suggesting that local application of PDGF may overcome the negative effects of diabetes and promote fracture healing. To test this hypothesis, low (22 mg) and high (75 ug) doses of recombinant human PDGF-BB (rhPDGF-BB) were applied directly to femur fracture sites in BB Wistar diabetic rats that were then compared to untreated or vehicle-treated animals. rhPDGF-BB treatment significantly increased early callus cell proliferation compared to that in control specimens. Low dose rhPDGF-BB treatment significantly increased callus peak torque values (p < 0.05) at 8 weeks after fracture as compared to controls. High dose rhPDGF-BB treatment increased callus bone area at 12 weeks postfracture. These data indicate that rhPDGF-BB treatment ameliorates the effects of diabetes on fracture healing by promoting early cellular proliferation that ultimately leads to more bone formation. Local application of rhPDGF-BB may be a new therapeutic approach to treat diabetes-impaired fracture healing. ß

show abstract

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Recombinant human platelet‐derived growth factor BB (rhPDGF‐BB) and beta‐tricalcium phosphate/collagen matrix enhance fracture healing in a diabetic rat model

Al-Zube

Breitbart

O’Connor

et al. 2009

Journal Orthopaedic Research

View full text Add to dashboard Cite

show abstract

“…The mechanical properties of the DM fracture callus have been evaluated in a number of rat fracture models [3,6,11,25]. Wray and Strunkle first observed a decrease in the breaking strength at four weeks of fractured tibiae from DM animals compared to control animals [25].…”

Section: Discussionmentioning

confidence: 99%

“…Wray and Strunkle first observed a decrease in the breaking strength at four weeks of fractured tibiae from DM animals compared to control animals [25]. Unfortunately, these studies neglected to normalize for individual variations in the geometry of the fracture callus.…”

Section: Discussionmentioning

confidence: 99%

Low‐intensity pulsed ultrasound increases the fracture callus strength in diabetic BB Wistar rats but does not affect cellular proliferation

Gebauer

Lin

Beam

et al. 2002

Journal Orthopaedic Research

View full text Add to dashboard Cite

Type I diabetes mellitus (DM) is associated with impaired fracture healing. Specifically, DM affects multiple phases of fracture healing including early cellular proliferation and late phases resulting in inferior biomechanical properties. Recent studies demonstrated the utility of pulsed low-intensity ultrasound (US) to facilitate fracture healing. The current study evaluated the effects of daily application of US on mid-diaphyseal femoral fractures in DM and non-DM BB Wistar rats. Immunohistochemical staining for PCNA was used to evaluate cellular proliferation at 2, 4, and 7 days post-fracture. In concordance with previous findings, DM fracture callus demonstrated decreased cellular proliferation. Importantly, the application of US did not significantly alter the proliferation in either DM or control groups. However, mechanical testing revealed significantly greater torque to failure and stiffness in US-treated DM versus non-US-treated DM groups at six weeks post-fracture. Despite the inability of US to affect the early proliferative phase of fracture healing, its application clearly results in improved mechanical properties during the late phases of healing. These findings suggest a potential role of US as an adjunct for DM fracture treatment.

show abstract

Involvement of Wnt4 signaling during myogenic proliferation and differentiation of skeletal muscle

Takata

Terada

Oka

et al. 2007

Developmental Dynamics

View full text Add to dashboard Cite

The effect of experimental diabetes upon the breaking strength of the healing fracture in the rat

Cited by 21 publications

References 6 publications

Recombinant human platelet‐derived growth factor BB (rhPDGF‐BB) and beta‐tricalcium phosphate/collagen matrix enhance fracture healing in a diabetic rat model

Recombinant human platelet‐derived growth factor BB (rhPDGF‐BB) and beta‐tricalcium phosphate/collagen matrix enhance fracture healing in a diabetic rat model

Low‐intensity pulsed ultrasound increases the fracture callus strength in diabetic BB Wistar rats but does not affect cellular proliferation

Involvement of Wnt4 signaling during myogenic proliferation and differentiation of skeletal muscle

Contact Info

Product

Resources

About