The effect of explant origin and collection season on stilbene biosynthesis in cell cultures of Vitis amurensis Rupr.

Tyunin, A. P.; Suprun, Andrey R.; Nityagovsky, Nikolay N.; Manyakhin, A. Y.; Karetin, Yu. A.; Dubrovina, Alexandra S.; Kiselev, Konstantin V.

doi:10.1007/s11240-018-1490-x

Cited by 19 publications

(13 citation statements)

References 37 publications

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“…In the PjSTS3-transgenic calli, the total stilbene content increased up to 3.1 mg/g DW and stilbene production up to 25.4 mg/L compared to the KA0 calli (Table 1). A comparison with the previously known stilbene content in different other stilbene sources [1] showed that stilbene content in the transgenic grapevine cells bearing spruce STS genes reached a considerably higher level of stilbene accumulation than in nontransgenic cell lines obtained from grapevine organs with low stilbene content [26,27] or grapevine leaves without treatments [28][29][30][31]. Also, in our PjSTS3-transgenic calli, the stilbene level was higher compared with that in grapevine cell lines overexpressing STS genes from V. amurensis [11,12].…”

Section: Individual Stilbene Content (% Dry Wt) In the Ka0 Zst1-1 2 3; Zst2-1 -2 -3; Zst3-1 -2 And -3 Cell Lines Overexpressing The Pjstssupporting

confidence: 47%

“…Also, in our PjSTS3 ‐transgenic calli, the stilbene level was higher compared with that in grapevine cell lines overexpressing STS genes from V. amurensis . The reached stilbene level in PjSTS gene overexpressing cells was close to the stilbene content in the grapevine cell lines obtained from grapevine organs with high stilbene content and grapevine leaves after UV‐C‐treatment . However, the stilbene level in PjSTS gene overexpressing cells was significantly lower than that obtained after overexpression of Agrobacterium rol genes in grapevine cell cultures or the content of stilbenes in the vine stems .…”

Section: Resultsmentioning

confidence: 50%

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Effect of spruce PjSTS1a, PjSTS2, or PjSTS3 gene overexpression on stilbene biosynthesis in callus cultures of Vitis amurensis Rupr

Suprun

Ogneva

Dubrovina

et al. 2019

Biotech and App Biochem

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Stilbenes are natural compounds protecting plants against microbial pathogens and known to possess valuable biologically active properties. In the present study, we established transgenic grapevine callus cell cultures overexpressing three stilbene synthase (STS) genes of spruce Picea jezoensis PjSTS1a, PjSTS2, and PjSTS3. Transformation of Vitis amurensis calli with the PjSTS1a, PjSTS2, and PjSTS3 genes significantly increased total content of stilbenes in 3.6–6, 2.5–2.9, and 4.1–16.1 times, respectively, in comparison with the control calli. The most pronounced positive effect on the accumulation of stilbenes was observed for the PjSTS3‐overexpressing calli where the total content of stilbenes was increased up to 3.1 mg/g DW, and the stilbene production reached 25.4 mg/L. These values were higher than those achieved for the grapevine callus cell cultures overexpressing three STS genes from V. amurensis. Thus, transformation of grapevine cell cultures with spruce STS genes with a relatively low degree of homology to the endogenous VaSTSs is a more effective strategy for induction of plant secondary metabolite biosynthesis than using the grapevine genes for the overexpression experiments.

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Section: Individual Stilbene Content (% Dry Wt) In the Ka0 Zst1-1 2 3; Zst2-1 -2 -3; Zst3-1 -2 And -3 Cell Lines Overexpressing The Pjstssupporting

confidence: 47%

Section: Resultsmentioning

confidence: 50%

Effect of spruce PjSTS1a, PjSTS2, or PjSTS3 gene overexpression on stilbene biosynthesis in callus cultures of Vitis amurensis Rupr

Suprun

Ogneva

Dubrovina

et al. 2019

Biotech and App Biochem

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“…The V7 callus culture was established in 2017 from young stems of the mature V. amurensis plants as described [ 39 ]. A 2.0 g amount of V7 cell culture (each callus was weighed using an electronic balance) was cultured in 50 mL of liquid Murashige and Skoog-modified W B/A medium supplemented with 0.5 mg/L 6-benzylaminopurine (B) and 2 mg/L α-naphthaleneacetic acid constantly stirring in the orbital shaker in the dark for 7 days [ 40 ].…”

Section: Methodsmentioning

confidence: 99%

The Influence of the Grapevine Bacterial and Fungal Endophytes on Biomass Accumulation and Stilbene Production by the In Vitro Cultivated Cells of Vitis amurensis Rupr.

Aleynova

Suprun

Nityagovsky

et al. 2021

Plants

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Plant endophytes are known to alter the profile of secondary metabolites in plant hosts. In this study, we identified the main bacterial and fungal representatives of the wild grape Vitis amurensis Rupr. microbiome and investigated a cocultivation effect of the 14 endophytes and the V. amurensis cell suspension on biomass accumulation and stilbene biosynthesis. The cocultivation of the V. amurensis cell culture with the bacteria Agrobacterium sp., Bacillus sp., and Curtobacterium sp. for 2 weeks did not significantly affect the accumulation of cell culture fresh biomass. However, it was significantly inhibited by the bacteria Erwinia sp., Pantoea sp., Pseudomonas sp., and Xanthomonas sp. and fungi Alternaria sp., Biscogniauxia sp., Cladosporium sp., Didymella sp. 2, and Fusarium sp. Cocultivation of the grapevine cell suspension with the fungi Didymella sp. 1 and Trichoderma sp. resulted in cell death. The addition of endophytic bacteria increased the total stilbene content by 2.2–5.3 times, while the addition of endophytic fungi was more effective in inducing stilbene accumulation by 2.6–16.3 times. The highest content of stilbenes in the grapevine cells cocultured with endophytic fungi was 13.63 and 13.76 mg/g of the cell dry weight (DW) after cultivation with Biscogniauxia sp. and Didymella sp. 2, respectively. The highest content of stilbenes in the grapevine cells cocultured with endophytic bacteria was 4.49 mg/g DW after cultivation with Xanthomonas sp. The increase in stilbene production was due to a significant activation of phenylalanine ammonia lyase (PAL) and stilbene synthase (STS) gene expression. We also analyzed the sensitivity of the selected endophytes to eight antibiotics, fluconazole, and trans-resveratrol. The endophytic bacteria were sensitive to gentamicin and kanamycin, while all selected fungal strains were resistant to fluconazole with the exception of Cladosporium sp. All endophytes were tolerant of trans-resveratrol. This study showed that grape endophytes stimulate the production of stilbenes in grape cell suspension, which could further contribute to the generation of a new stimulator of stilbene biosynthesis in grapevine or grape cell cultures.

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“…The KA-0 cell culture was used as a control in all further experiments. The V7 callus culture were established in 2017 from young stems of the mature V. amurensis plants as described [48]. After the transformation, the calli were cultivated for a 4-month period in the presence of 10-20 mg/L of Km to select transgenic cells and for a 6-month period in the presence of 250 mg/L of Cf to suppress the bacteria.…”

Section: Generation Of Transgenic Grapevine Cell Culturesmentioning

confidence: 99%

The Grapevine Calmodulin-Like Protein Gene CML21 Is Regulated by Alternative Splicing and Involved in Abiotic Stress Response

Aleynova

Kiselev

Ogneva

et al. 2020

IJMS

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Calmodulin-like proteins (CMLs) represent a large family of plant calcium sensor proteins involved in the regulation of plant responses to environmental cues and developmental processes. In the present work, we identified four alternatively spliced mRNA forms of the grapevine CML21 gene that encoded proteins with distinct N-terminal regions. We studied the transcript abundance of CML21v1, CML21v2, CML21v3, and CML21v4 in wild-growing grapevine Vitis amurensis Rupr. in response to desiccation, heat, cold, high salinity, and high mannitol stress using quantitative real-time RT-PCR. The levels of all four splice variants of VaCML21 were highly induced in response to cold stress. In addition, VaCML21v1 and VaCML21v2 forms were highly modulated by all other abiotic stress treatments. Constitutive expression of VaCML21v2 and VaCML21v4 improved biomass accumulation of V. amurensis callus cell cultures under prolonged low temperature stress. Heterologous expression of the grapevine CML21v2 and VaCML21v4 splice variants in Arabidopsis improved survival rates of the transgenic plants after freezing. The VaCML21v2 overexpression enhanced activation of the cold stress-responsive marker genes AtDREB1A and AtDREB2A, while VaCML21v4 overexpression—AtCOR47, AtRD29A, AtRD29B, and AtKIN1 genes after freezing stress in the transgenic Arabidopsis. The results indicate that the grapevine CML21 gene acts as a positive regulator in the plant response to cold stress. The detected variety of CML21 transcripts and their distinct transcriptional responses suggested that this expansion of mRNA variants could contribute to the diversity of grapevine adaptive reactions.

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The effect of explant origin and collection season on stilbene biosynthesis in cell cultures of Vitis amurensis Rupr.

Cited by 19 publications

References 37 publications

Effect of spruce PjSTS1a, PjSTS2, or PjSTS3 gene overexpression on stilbene biosynthesis in callus cultures of Vitis amurensis Rupr

Effect of spruce PjSTS1a, PjSTS2, or PjSTS3 gene overexpression on stilbene biosynthesis in callus cultures of Vitis amurensis Rupr

The Influence of the Grapevine Bacterial and Fungal Endophytes on Biomass Accumulation and Stilbene Production by the In Vitro Cultivated Cells of Vitis amurensis Rupr.

The Grapevine Calmodulin-Like Protein Gene CML21 Is Regulated by Alternative Splicing and Involved in Abiotic Stress Response

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