The effect of exposure to chlorhexidine gluconate in vitro and in vivo on in vitro adhesion of Candida albicans to buccal epithelial cells from diabetic and non‐diabetic subjects

Darwazeh, A. M. G.; Lamey, P J; MacFarlane, T. W.; McCuish, A. C.

doi:10.1111/j.1600-0714.1994.tb01100.x

Cited by 33 publications

(28 citation statements)

References 19 publications

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“…The CHX mouthwash has the highest efficiency to reduce germ tube of C. albicans. Similar results were reported by others and showed that CHX mouthwashes reduced C. albicans adhesion to BEC and germ tube formation significantly (Darwazeh et al, 1994;Ellepola and Samaranayake, 2000;Pizzo et al, 2001). CHX decreased epithelial-cell membrane-lipid packing order, particularly in superficial regions, similar to antifungal drug used for detachment of adherent yeasts (Audus et al, 1992).…”

Section: Resultssupporting

confidence: 78%

Candida inhibitory effects of six commercial mouthwashes

Aroonrerk

Dhanesuan

2007

Ann. Microbiol.

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The Candida inhibitory activities of commercial mouthwashes with various active ingredients -fluoride (FLO), cethylpyridinium chloride (CPC), chlorhexidine gluconate (CHX), triclosan (TRI) and herbal extracts: Twin Lotus (TLO) and Herbric concentrated (HBC) -were determined. The Candida activities included growth, germ tube formation and adhesion of Candida albicans to human buccal mucosa. This study showed that TRI, TLO and CHX mouthwashes had growth inhibitory effects to C. albicans with the MIC of 1/64, 1/64 and 1/32, respectively. CHX, TRI, TLO, HBC and CPC mouthwashes had ability to inhibit adhesion of C. albicans ATCC 10231 by approximately 85, 80, 70, 65 and 55%, respectively. Germ tube formation, or mycelial conversion, of C. albicans was inhibited by approximately 90, 85 and 80% after treatment with 20% mouthwashes containing CHX, TRI and CPC, respectively. Fluoride mouthwash showed the weakest in all inhibitory activities. These results suggested that CHX and triclosan mouthwashes were effective in reducing oral Candida activities.

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Section: Resultssupporting

confidence: 78%

Candida inhibitory effects of six commercial mouthwashes

Aroonrerk

Dhanesuan

2007

Ann. Microbiol.

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“…other than C. albicans, such as C. glabrata, Candida tropicalis and C. krusei [13,17]. It has also been shown to have superior efficacy against Candida biofilms compared with FLZ in vitro and in vivo [13,[18][19][20][21]. Furthermore, it can be used to impregnate denture liners to act as a long-term self-release drug carrier [22].…”

Section: Introductionmentioning

confidence: 97%

Chlorhexidine is a highly effective topical broad-spectrum agent against Candida spp.

Salim

Moore

Silikas

et al. 2013

International Journal of Antimicrobial Agents

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“…The antifungal effect of chlorhexidine gluconate has been demonstrated in several in vivo and in vitro trials, including some related to Candida infection [9]. Further, it has been demonstrated that exposure of either Candida isolates or buccal epithelial cells to 0.2% chlorhexidine gluconate profoundly suppressed their ability to adhere to buccal epithelial cells in healthy individuals [10] as well as in diabetics [11]. Moreover, pretreatment of acrylic dentures with 2% chlorhexidine gluconate has also been shown to suppress the adhesion of Candida to the denture's acrylic surfaces [12].…”

Section: Introductionmentioning

confidence: 99%

Effects of Subtherapeutic Concentrations of Chlorhexidine Gluconate on Germ Tube Formation of Oral Candida

Ellepola

Joseph²,

Khan

2011

Med Princ Pract

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Objective: The main objective of this study was to investigate the effect of brief exposure to subtherapeutic concentrations of chlorhexidine gluconate on germ tube formation of Candida albicans isolates obtained from smokers, diabetics, asthmatics using steroid inhalers and healthy individuals. Materials and Methods: Forty isolates of C. albicans were used in this study. All these isolates were quantified for germ tube formation without exposure to the drug and were used as the control group for data analysis. Isolates were also exposed to three subtherapeutic concentrations of chlorhexidine gluconate (0.00125, 0.0025 and 0.005%) for 30 min (limited exposure); the antiseptic was then removed and germ tube formation of these isolates was quantified microscopically following incubation in a germ tube-inducing medium. Results: Compared with the unexposed controls, brief exposure to all concentrations of chlorhexidine gluconate suppressed the ability of the C. albicans isolates to form germ tubes in increasing order by 13.72% (p < 0.001 to p = 0.02), 46.16% (p < 0.001) and 72.46% (p <0.001). Conclusions: These findings show that brief exposure to subtherapeutic concentrations of chlorhexidine gluconate may modulate germ tube formation of C. albicans isolates, thereby suppressing their pathogenicity, and further elucidate the pharmacodynamic mechanisms by which chlorhexidine gluconate may operate in vivo.

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The effect of exposure to chlorhexidine gluconate in vitro and in vivo on in vitro adhesion of Candida albicans to buccal epithelial cells from diabetic and non‐diabetic subjects

Cited by 33 publications

References 19 publications

Candida inhibitory effects of six commercial mouthwashes

Candida inhibitory effects of six commercial mouthwashes

Chlorhexidine is a highly effective topical broad-spectrum agent against Candida spp.

Effects of Subtherapeutic Concentrations of Chlorhexidine Gluconate on Germ Tube Formation of Oral <i>Candida</i>

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