The Effect of Flow Rate on the Ionized Calcium Concentration of Human Parotid Saliva

Lagerlöf, Folke; Ekstrand, Jan

doi:10.1159/000260588

Cited by 21 publications

(8 citation statements)

References 8 publications

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“…Many studies revealed the relationship between the amount and components of saliva and some diseases. As the flow rate in parotid saliva of normal people grew more and more in amount, the concentrations of the total protein, calcium and sodium increased 2,3) . The flow rate of the patients suffering from chronic parotitis decreased in comparison with that of normal healthy people 4) .…”

Section: Introductionmentioning

confidence: 99%

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Lipids in Human Parotid Saliva with Regard to Caries Experience

Tomita¹,

Miyake²,

Yamanaka

2008

J. Oleo Sci.

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It has been reported that saliva may play an important role in the prevention and development of enamel caries and that both lipids and protein contents in saliva may be relevant to this role. This study examined the lipid and protein levels in saliva from individuals differing in caries experience. Female subjects (20 to 21 years old) were used divided equally into two groups, caries-susceptible group (CSG) and caries-resistant group (CRG). Stimulated parotid saliva and stimulated whole saliva were collected from the subjects. After centrifugation, each saliva sample was analyzed for the concentrations of lipids and proteins and for the compositions of lipids and fatty acids. The lipid and protein contents in parotid saliva increased in proportion to increase of the flow rate. The lipid content was slightly correlated with the protein one (r = 0.33). Total lipid and protein concentrations were higher in the samples from CSG than those from CRG. The lipid composition was similar in the samples from the two groups; more than half in total lipids was neutral lipids, followed by glycolipids and phospholipids. Neutral lipids and free fatty acid and triacylglyceride concentrations were significantly higher in the samples from CSG than those from CRG (p<0.01 for each). Also stearic, linoleic and docosahexaenoic acids were significantly higher in the former group than the latter one (p<0.05 and p<0.01). In summary, the lipid concentrations in parotid saliva from caries susceptible subjects were higher than those from caries resistant ones, and the difference in fatty acid composition was detected between them. The variations in the lipid levels and fatty acid composition may be associated with those in caries development.

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Section: Introductionmentioning

confidence: 99%

“…Oleo Sci. 57, (2) 115-121 (2008) with the mucous proteins 2,3,22) . As mentioned above, protein and lipids, which have the high molecular weight, reduce the reabsorption of them through the acinar cells in parotid gland, so that lipids and protein in parotid saliva showed upward tendency of concentration in proportion to the increase of the flow rate.…”

mentioning

confidence: 99%

Lipids in Human Parotid Saliva with Regard to Caries Experience

Tomita¹,

Miyake²,

Yamanaka

2008

J. Oleo Sci.

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“…Previous studies have shown that one part of salivary calcium secretion takes place bound to proteins like -amylase, which is secreted in zymogen granula by exocytosis. The other part is secreted as free Ca 2+ (Lagerlof and Ekstrand 1982).…”

Section: Introductionmentioning

confidence: 99%

Identification and subcellular localization of paracellin-1 (claudin-16) in human salivary glands

Kriegs

Homann

Kinne-Saffran

et al. 2007

Histochem Cell Biol

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Salivary calcium plays an important role in the pathogenesis of dental caries and the bio-mineralization of dental enamel and exposed dentin. The cellular and molecular basis of calcium secretion by the human salivary glands is, however, poorly understood. Recently a transcellular transport of calcium by the acinus cells has been proposed. In this paper we looked for evidence for paracellular calcium transport by investigating the presence and cellular localization of paracellin-1 (claudin-16) that has been implied in paracellular magnesium and calcium transport in the kidney. At the mRNA level, using RT-PCR with primers of appropriate sequence, paracellin-1 mRNA could be found in human Glandula parotis, Glandula submandibularis, Glandula labialis and Glandula sublingualis samples. In addition, a splice variant was detected in three out of 15 glands consisting of exons one and five of the paracellin gene. In immunohistochemical studies paracellin-1 colocalised in the salivary excretory ducts with the tight junction proteins ZO-1 and occludin suggesting a potential role in paracellular calcium and magnesium transport. In the acini no such colocalisation was observed; paracellin was instead detected at the basal poles of the cells, between cells of the same acinus as well as between cells of neighboring acini. At this location paracellin-1 might act as selectivity filter for the paracellular movement of ions and water during stimulated secretion. Thus, both in the ducts and in the acini a paracellular transport of calcium appears possible. Whether it occurs at all and the extent to which it contributes to the overall salivary calcium secretion remains, however, to be determined.

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“…Since total and ionized calcium and protein tend to be secreted concomitantly by adult rat and human parotid and submandibular glands (Wallach and Schramm, 1971;Lagerlof and Ekstrand, 1982;Maier et al, 1983), the P-adrenoceptors may therefore play the most important role in the regulation of calcium secretion. The 13-adrenergic receptor contains both the ligand binding site and the site responsible for mediating stimulation of adenylate cyclase activity.…”

Section: Discussionmentioning

confidence: 99%

Developmental Changes in the Volumes, Protein, and Some Electrolyte Concentrations of Male and Female Rat Submandibular Saliva Secreted in Response to Methoxamine and Pilocarpine

et al. 1987

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Saliva secreted in response to methoxamine and pilocarpine was collected from the cannulated ducts of the submandibular glands of male and female rats at weekly intervals from two to 10 weeks of age. It was analyzed for volume and for concentrations of protein, potassium, calcium, and inorganic phosphate. Following the collection of saliva, the submandibular glands were removed and weighted. The wet weights of the glands increased substantially up to seven weeks of age and then reached almost plateau values in both sexes. The salivary volumes secreted in response to both agents in both sexes were positively correlated with the gland weights, except that after five to six weeks of age there was no correlation between gland weight and methoxamine-stimulated salivary volume. The concentrations of protein, potassium, and inorganic phosphate were inversely related to the flow rates only at relatively low rates of flow. The concentration of calcium was positively correlated with the protein concentration and was independent of the nature of the stimulus and of sex differences during postnatal development.

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The Effect of Flow Rate on the Ionized Calcium Concentration of Human Parotid Saliva

Cited by 21 publications

References 8 publications

Lipids in Human Parotid Saliva with Regard to Caries Experience

Lipids in Human Parotid Saliva with Regard to Caries Experience

Identification and subcellular localization of paracellin-1 (claudin-16) in human salivary glands

Developmental Changes in the Volumes, Protein, and Some Electrolyte Concentrations of Male and Female Rat Submandibular Saliva Secreted in Response to Methoxamine and Pilocarpine

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