The effect of freeze-drying and storage on lysozyme activity, lactoferrin content, superoxide dismutase activity, total antioxidant capacity and fatty acid profile of freeze-dried human milk

Martysiak‐Żurowska, Dorota; Rożek, Patrycja; Puta, Małgorzata

doi:10.1080/07373937.2020.1824188

Cited by 18 publications

(14 citation statements)

References 44 publications

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“…The only human milk component that showed a significant decrease was adiponectin, which indicated the possibility of a genuine effect of HPP + Lyo on this component of human milk ( Figure 3 ). Other authors have also reported good results in terms of the nutritional content of lyophilized human milk [ 24 , 26 ]. Oliveira et al demonstrated preservation of osmolality and stability of nutritional content in freeze-dried human milk samples that were subsequently stored for 3 and 6 months [ 26 ].…”

Section: Discussionmentioning

confidence: 99%

“…Those components remained stable regardless of time (6 weeks) and temperature (5 °C and 25 °C) of storage. The authors observed the decrease only for superoxidase dismutase activity, but it was a significantly lower decline than is observed in frozen raw human milk [ 24 ]. Results of our study and from the other authors emphasized the high potential of utilizing lyophilization for donor milk storage at human milk banks.…”

Section: Discussionmentioning

confidence: 99%

“…Freeze-drying of human milk causes no degradation of most of the nutrients, vitamins, or proteins, and the taste and flavor remain unchanged following rehydration [ 21 , 22 , 23 ]. Apart from the findings by Salcedo et al (2015) on the successful reduction of germs in breast-milk after freeze-drying, it was shown that storage of freeze-dried milk did not show a significant influence upon several biological properties of human milk such as total antioxidant capacity and fatty acid profile of milk [ 21 , 22 , 23 , 24 ]. The storage and transport of milk powder is also considerably less complicated in the hospital setting and makes possible longer storage than in the case of pasteurized milk, which must be frozen [ 21 , 22 , 25 ].…”

Section: Introductionmentioning

confidence: 99%

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Combination of High-Pressure Processing and Freeze-Drying as the Most Effective Techniques in Maintaining Biological Values and Microbiological Safety of Donor Milk

Strom

Barbarska

Pawlikowska

et al. 2021

IJERPH

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Background: Human milk banks have a pivotal role in provide optimal food for those infants who are not fully breastfeed, by allowing human milk from donors to be collected, processed and appropriately distributed. Donor human milk (DHM) is usually preserved by Holder pasteurization, considered to be the gold standard to ensure the microbiology safety and nutritional value of milk. However, as stated by the European Milk Banking Association (EMBA) there is a need to implement the improvement of the operating procedure of human milk banks including preserving and storing techniques. Aim: The purpose of this study was to assess the effectiveness and safety of the selected new combination of methods for preserving donor human milk in comparison with thermal treatment (Holder pasteurization). Methods: We assessed (1) the concentration of bioactive components (insulin, adiponectin, leptin, activity of pancreatic lipase, and hepatocyte growth factor) and (2) microbiological safety in raw and pasteurized, high-pressure processed and lyophilization human breast milk. Results: The combination of two techniques, high-pressure processing and freeze-drying, showed the best potential for preserving the nutritional value of human milk and were evaluated for microbiological safety. Microbiological safety assessment excluded the possibility of using freeze-drying alone for human milk sample preservation. However, it can be used as a method for long-term storage of milk samples, which have previously been preserved via other processes. Conclusion: The results show that high-pressure treatment is the best method for preservation that ensures microbiological safety and biological activity but subsequent freeze -drying allowed long-term storage without loss of properties.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Combination of High-Pressure Processing and Freeze-Drying as the Most Effective Techniques in Maintaining Biological Values and Microbiological Safety of Donor Milk

Strom

Barbarska

Pawlikowska

et al. 2021

IJERPH

View full text Add to dashboard Cite

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“…Consequently, several studies have now reported on the effects of freeze-drying on DHM. No significant effect has been found on the protein profile, the oligosaccharides, or the fatty acid profile in human milk after freeze-drying [ 17 , 18 , 19 ]. Significantly, freeze-drying retains the lactoferrin content of human milk, but a decrease in lysozyme and antioxidants has been reported [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

The Effects of Thermal Pasteurisation, Freeze-Drying, and Gamma-Irradiation on the Antibacterial Properties of Donor Human Milk

Blackshaw

Valtchev

et al. 2021

Foods

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The most common pasteurisation method used by human milk banks is Holder pasteurisation. This involves thermal processing, which can denature important proteins and can potentially reduce the natural antimicrobial properties found in human milk. This study assesses the application of a hybrid method comprised of freeze-drying followed by low-dose gamma-irradiation for nonthermal donor human milk pasteurisation. Freeze-drying donor human milk followed by gamma-irradiation at 2 kGy was as efficient as Holder pasteurisation in the reduction of bacterial inoculants of Staphylococcus aureus (106 cfu/mL) and Salmonella typhimurium (106 cfu/mL) in growth inhibition assays. These assays also demonstrated that human milk naturally inhibits the growth of bacterial inoculants S. aureus, S. typhimurium, and Escherichia coli. Freeze drying (without gamma-irradiation) did not significantly reduce this natural growth inhibition. By contrast, Holder pasteurisation significantly reduced the milk’s natural antimicrobial effect on S. aureus growth after 6 h (−19.8% p = 0.01). Freeze-dried and then gamma-irradiated donor human milk showed a strong antimicrobial effect across a dose range of 2–50 kGy, with only a minimal growth of S. aureus observed after 6 h incubation. Thus, a hybrid method of freeze-drying followed by 2 kGy of gamma-irradiation preserves antimicrobial properties and enables bulk pasteurisation within sealed packaging of powderised donor human milk. This work forwards a goal of increasing shelf life and simplifying storage and transportation, while also preserving functionality and antimicrobial properties.

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“…However, some studies showed that, as a result of freeze-drying, food materials showed a loss of essential nutritional contents, such as vitamins and minerals [ 6 , 7 ]. Low antioxidant activity is also reported in fruits dried by freeze-drying [ 8 ]. With an increase in demand of efficient and cheap drying techniques, heat-drying technologies have gained a lot of R&D efforts to develop cheaper and convenient methods to dry plant products.…”

Section: Introductionmentioning

confidence: 99%

Postharvest Drying Techniques Regulate Secondary Metabolites and Anti-Neuroinflammatory Activities of Ganoderma lucidum

et al. 2021

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Ganoderma lucidum extract is a potent traditional remedy for curing various ailments. Drying is the most important postharvest step during the processing of Ganoderma lucidum. The drying process mainly involves heat (36 h at 60 °C) and freeze-drying (36 h at −80 °C). We investigated the effects of different postharvest drying protocols on the metabolites profiling of Ganoderma lucidum using GC-MS, followed by an investigation of the anti-neuroinflammatory potential in LPS-treated BV2 microglial cells. A total of 109 primary metabolites were detected from heat and freeze-dried samples. Primary metabolite profiling showed higher levels of amino acids (17.4%) and monosaccharides (8.8%) in the heat-dried extracts, whereas high levels of organic acids (64.1%) were present in the freeze-dried samples. The enzymatic activity, such as ATP-citrate synthase, pyruvate kinase, glyceraldehyde-3-phosphatase dehydrogenase, glutamine synthase, fructose-bisphosphate aldolase, and D-3-phosphoglycerate dehydrogenase, related to the reverse tricarboxylic acid cycle were significantly high in the heat-dried samples. We also observed a decreased phosphorylation level of the MAP kinase (Erk1/2, p38, and JNK) and NF-κB subunit p65 in the heat-dried samples of the BV2 microglia cells. The current study suggests that heat drying improves the production of ganoderic acids by the upregulation of TCA-related pathways, which, in turn, gives a significant reduction in the inflammatory response of LPS-induced BV2 cells. This may be attributed to the inhibition of NF-κB and MAP kinase signaling pathways in cells treated with heat-dried extracts.

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The effect of freeze-drying and storage on lysozyme activity, lactoferrin content, superoxide dismutase activity, total antioxidant capacity and fatty acid profile of freeze-dried human milk

Cited by 18 publications

References 44 publications

Combination of High-Pressure Processing and Freeze-Drying as the Most Effective Techniques in Maintaining Biological Values and Microbiological Safety of Donor Milk

Combination of High-Pressure Processing and Freeze-Drying as the Most Effective Techniques in Maintaining Biological Values and Microbiological Safety of Donor Milk

The Effects of Thermal Pasteurisation, Freeze-Drying, and Gamma-Irradiation on the Antibacterial Properties of Donor Human Milk

Postharvest Drying Techniques Regulate Secondary Metabolites and Anti-Neuroinflammatory Activities of Ganoderma lucidum

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