The effect of germination on seed trypsin inhibitors in <i>Vicia faba</i> and <i>Cicer arietinum</i>

Múzquiz, M.; Welham, Tracey; Altares, P.; Goyoaga, Carmen; Cuadrado, Carmen; Romero, C.; Guillamón, Eva; Domoney, Claire

doi:10.1002/jsfa.1695

Cited by 16 publications

(11 citation statements)

References 23 publications

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“…Moist heat treatment in the oilseed processing industry has been widely used commercially to inactivate mostly TIA in soybean 26. Moreover, the present chickpea TIA data are in agreement with those previously reported for two chickpea varieties determined with the same methodology 27. Regarding PI amounts determined by ELISA, the results for KTI were 5‐fold higher than BBI for defatted soybean meal (Table 2).…”

Section: Discussionsupporting

confidence: 91%

Recovery at the terminal ileum of some legume non‐nutritional factors in cannulated pigs

Rubio¹,

Pedrosa²,

Cuadrado³

et al. 2006

J Sci Food Agric

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In order to exert an effect, either local or systemic, putative beneficial dietary substances have to survive at least to some extent the digestive process within the gastrointestinal tract. For that purpose, five castrated male pigs (100 ± 2 kg mean live b.w.) fitted with T-shaped ileal cannulas were used to determine the recovery up to the terminal ileum of various non-nutritional factors (NNF) from legumes (defatted soybean, raw lupin, raw chickpea and raw or autoclaved kidney beans). Kidney bean lectin (PHA) recovery within the small intestine ranged between 2.5 and 4.8 mg from 5.96 mg ingested. Two different methods were used to determine ileal digestibility of protease inhibitors (PI) in pigs. According to the enzyme-linked immunosorbent assay (ELISA) method, apparent digestibility of Kunitz trypsin inhibitor and Bowman-Birk inhibitor for pigs fed the defatted soybean-based diet were 0 and 58%, respectively. On the other hand, the apparent digestibility of PI along the small intestine, expressed in terms of trypsin inhibitory activity, was 96, 98 and 95% of activity in the diet, while data expressed in terms of chymotrypsin inhibitory activity were 95, 99 and 79% of activity in the diet for defatted soybean, raw chickpea and autoclaved kidney bean diets respectively. Differences found in ileal digestibility values of PI using both methodologies, ELISA and enzymatic inhibition, might be explained by the detection of inactive forms by specific antibodies. Intestinal apparent digestibility of phytate was 0% for defatted soybean and autoclaved kidney bean diets, whereas values for raw lupin and chickpea diets were 4.1 and 24.5%, respectively. In conclusion, significant amounts of several NNF survived the passage through the stomach and small intestine of cannulated pigs, which might have biological relevance.

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Section: Discussionsupporting

confidence: 91%

Recovery at the terminal ileum of some legume non‐nutritional factors in cannulated pigs

Rubio¹,

Pedrosa²,

Cuadrado³

et al. 2006

J Sci Food Agric

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“…Similar works have reported ratios equal to 2:1 for two Spanish varieties of broad beans (Muzquiz et al . ). In that work, the results of vicine content (4.74 and 4.16 mg/g) were similar to that obtained for the raw cotyledons analyzed here; however, the content of convicine for the two Spanish varieties was higher than for the 10 Mexican varieties of V. faba L. (2.32 and 2.03 mg/g) (Goyoaga et al .…”

Section: Resultsmentioning

confidence: 97%

Effect of Roasting and Boiling on the Content of Vicine, Convicine and L‐3,4‐dihydroxyphenylalanine in Vicia fabaL.

Cardador‐Martínez

Maya-Ocaña

Ortiz-Moreno

et al. 2012

Journal of Food Quality

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The pyrimidine glycosides, vicine and convicine, are compounds of the genus Vicia, whereas their aglycone derivatives, divicine and isouramil, are responsible for favism occurrence, and L‐3,4‐dihydroxyphenylalanine (L‐DOPA) is a nonprotein amino acid precursor of dopamine in healthy neurons. The present work evaluated the effect of thermal treatment, which consisted of roasting and boiling, on the content of vicine, convicine and L‐DOPA in mature cotyledons of 10 varieties of Mexican Vicia faba L. and determined the concentration of glycosides and L‐DOPA by high‐performance liquid chromatography. The raw cotyledons showed 2.88–6.10, 0.63–1.68 and 0.28–0.44 vicine, convicine and L‐DOPA, respectively. The 12 and 40% and 30 and 61% decrease in vicine and convicine content, respectively, were observed to be the effect of roasting and boiling the samples. L‐DOPA was totally removed by both thermal processes. In all analyzed samples, vicine was the main compound; however, there were significant differences between varieties and thermal treatments. Practical Applications Vicine and convicine glycosides are strongly implicated in favism, a hemolytic anemia in humans, and in poor performance of laying hens. There are reports of decreased egg weight, increase in the fragility of the yolk and a number of blood stains, and decreased fertility and hatchability of eggs. The characterization of new varieties of faba bean in their content of potentially toxic glycosides allows selection of those with the lowest possible content of such compounds. Also, the application of heat treatments that reduce glycosides will allow the use of seeds or flour to develop harmless food and feed ingredients.

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“…polyphyllus should simulate a biological invasion into the established ruderal plant community and prevent the emitted alkaloids from disturbing germination of the other plants (Muzquiz et al. ).…”

Section: Methodsmentioning

confidence: 99%

Soil biota of different size classes change the impact of soil compaction on a plant community

Beck

Wurst

2013

Applied Vegetation Science

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Question Does a reduction in size class of soil biota aggravate the impacts of soil compaction on plant communities? Location Controlled mesocosm study with a European ruderal plant community exposed to soil compaction and soil biota communities of different size classes. Methods We evaluated the individual and combined effects of soil compaction and soil biota communities of different size classes (<2000, <212, <20 μm, natural control) on the structure and productivity of a ruderal plant community, and the establishment of a neophyte (Lupinus polyphyllus) in a full‐factorial mesocosm study. Results Soil compaction reduced the productivity of the plant community, but the negative effect decreased in magnitude as the size class of the soil biota community was reduced. Consistently, shoot growth of the dominant grasses was most reduced by soil compaction when bigger soil biota were present; the shoot growth of forbs showed an opposite pattern. In contrast, legumes seem to profit from soil compaction independent of the size class of the soil biota community, and were detrimentally affected only in the natural control soil. Establishment of the neophyte L. polyphyllus was best under natural soil conditions and was not affected by soil compaction. Conclusions A reduction in soil biota size due to intensified management may change the impacts of soil compaction on plant communities. The results indicate that soil compaction has the most detrimental effect on primary productivity in soil communities where a range of size classes of soil biota is present and the functional complexity of the soil biota community is therefore high.

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The effect of germination on seed trypsin inhibitors in Vicia faba and Cicer arietinum

Cited by 16 publications

References 23 publications

Recovery at the terminal ileum of some legume non‐nutritional factors in cannulated pigs

Recovery at the terminal ileum of some legume non‐nutritional factors in cannulated pigs

Effect of Roasting and Boiling on the Content of Vicine, Convicine and L‐3,4‐dihydroxyphenylalanine in Vicia fabaL.

Soil biota of different size classes change the impact of soil compaction on a plant community

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