The effect of glow discharge plasma surface modification of polymers on the osteogenic differentiation of committed human mesenchymal stem cells

Mwale, Fackson; Wang, Hong; Nelea, Valentin; Luo, Li; Antoniou, John; Wertheimer, M. R.

doi:10.1016/j.biomaterials.2005.11.006

Cited by 89 publications

(74 citation statements)

References 29 publications

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“…22 The functional groups can covalently couple biomolecules, such as RGD tripeptide. 23 It increases immobilization of peptides and cell adhesion. The surface of the NH 3 plasma pretreatment was reported to be easier to anchor collagen.…”

Section: 45mentioning

confidence: 99%

Amide‐linkage formed between ammonia plasma treated poly(D,L‐lactide acid) scaffolds and bio‐peptides: Enhancement of cell adhesion and osteogenic differentiation in vitro

Zhong

et al. 2011

Biopolymers

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The surface characteristics of scaffolds for bone tissue engineering must support cell adhesion, migration, proliferation, and osteogenic differentiation. In the study, poly(D,L-lactide acid) (PDLLA) scaffolds were modified by combing ammonia (NH(3) ) plasma pretreatment with Gly-Arg-Gly-Asp-Ser (GRGDS)-peptides coupling technologies. The x-ray photoelectron spectroscopy (XPS) survey spectra showed the peak of N1s at the surface of NH(3) plasma pretreated PDLLA, which was further raised after GRGDS conjugation. Furthermore, N1s and C1s in the high-resolution XPS spectra revealed the presence of -C=N(imine), -C-NH-(amine), and -C=O-NH- (amide) groups. The GRGDS conjugation increased amide groups and decreased amine groups in the plasma-treated PDLLA. Confocal microscope and high performance liquid chromatography verified the anchored peptides after the conjugation process. Bone marrow mesenchymal stem cells were co-cultured with scaffolds. Fluorescent microscope and scanning electron microscope photographs revealed the best cell adhesion in NH(3) plasma pretreated and GRGDS conjugated scaffolds, and the least attachment in unmodified scaffolds. Real-time PCR demonstrated that expression of osteogenesis-related genes, such as osteocalcin, alkaline phosphatase, type I collagen, bone morphogenetic protein-2 and osteopontin, was upregulated in the single NH(3) plasma treated and NH(3) plasma pretreated scaffolds following GRGDS conjugation. The results show that NH(3) plasma treatment promotes the conjugation of GRGDS peptides to the PDLLA scaffolds via the formation of amide linkage, and combination of NH(3) plasma treatment and peptides conjugation may enhance the cell adhesion and osteogenic differentiation in the PDLLA scaffolds. © 2011 Wiley Periodicals, Inc. Biopolymers 95: 682-694, 2011.

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Section: 45mentioning

confidence: 99%

Amide‐linkage formed between ammonia plasma treated poly(D,L‐lactide acid) scaffolds and bio‐peptides: Enhancement of cell adhesion and osteogenic differentiation in vitro

Zhong

et al. 2011

Biopolymers

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“…For example, plasma surfacemodification (PSM) techniques such as radio frequency glow discharge have been tested to alter the surface functionalities and, therefore modulate protein adsorption [4] and cell adhesion to biomaterials [5]. Higher reproducibility in surface treatment and flexibility to obtain different surface characteristics, such as surface charge [6], energy [7] and chemistry [8] were achieved using PSM.…”

Section: Introductionmentioning

confidence: 99%

Modulating protein adsorption onto hydroxyapatite particles using different amino acid treatments

Lee

Loo

Van

et al. 2011

J. R. Soc. Interface.

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Hydroxyapatite (HA) is a material of choice for bone grafts owing to its chemical and structural similarities to the mineral phase of hard tissues. The combination of osteogenic proteins with HA materials that carry and deliver the proteins to the bone-defective areas will accelerate bone regeneration. The study investigated the treatment of HA particles with different amino acids such as serine (Ser), asparagine (Asn), aspartic acid (Asp) and arginine (Arg) to enhance the adsorption ability of HA carrier for delivering therapeutic proteins to the body. The crystallinity of HA reduced when amino acids were added during HA preparation. Depending on the types of amino acid, the specific surface area of the amino acid-functionalized HA particles varied from 105 to 149 m 2 g -1. Bovine serum albumin (BSA) and lysozyme were used as model proteins for adsorption study. The protein adsorption onto the surface of amino acid-functionalized HA depended on the polarities of HA particles, whereby, compared with lysozyme, BSA demonstrated higher affinity towards positively charged Arg-HA. Alternatively, the binding affinity of lysozyme onto the negatively charged Asp-HA was higher when compared with BSA. The BSA and lysozyme adsorptions onto the amino acid-functionalized HA fitted better into the Freundlich than Langmuir model. The amino acid-functionalized HA particles that had higher protein adsorption demonstrated a lower protein-release rate.

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“…carbon dioxide (CO 2 ), nitrogen (N 2 ) or ammonia (NH 3 )) and of monomer addition (e.g. hydrocarbons), the plasma technology allows formation of functionalized surfaces that are stable in aqueous environments [13][14][15][16][17][18]. Plasma technology is routinely used to prepare cell cultivation surfaces as substrates for cultivation of adherent cells.…”

Section: Introductionmentioning

confidence: 99%

Antibacterial burst-release from minimal Ag-containing plasma polymer coatings

Lischer

Körner

Balazs

et al. 2011

J. R. Soc. Interface.

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Biomaterials releasing silver (Ag) are of interest because of their ability to inhibit pathogenic bacteria including antibiotic-resistant strains. In order to investigate the potential of nanometre-thick Ag polymer (Ag/amino-hydrocarbon) nanocomposite plasma coatings, we studied a comprehensive range of factors such as the plasma deposition process and Ag cation release as well as the antibacterial and cytocompatible properties. The nanocomposite coatings released most bound Ag within the first day of immersion in water yielding an antibacterial burst. The release kinetics correlated with the inhibitory effects on the pathogens Pseudomonas aeruginosa or Staphylococcus aureus and on animal cells that were in contact with these coatings. We identified a unique range of Ag content that provided an effective antibacterial peak release, followed by cytocompatible conditions soon thereafter. The control of the in situ growth conditions for Ag nanoparticles in the polymer matrix offers the possibility to produce customized coatings that initially release sufficient quantities of Ag ions to produce a strong adjacent antibacterial effect, and at the same time exhibit a rapidly decaying Ag content to provide surface cytocompatibility within hours/days. This approach seems to be favourable with respect to implant surfaces and possible Ag-resistance/tolerance built-up.

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The effect of glow discharge plasma surface modification of polymers on the osteogenic differentiation of committed human mesenchymal stem cells

Cited by 89 publications

References 29 publications

Amide‐linkage formed between ammonia plasma treated poly(D,L‐lactide acid) scaffolds and bio‐peptides: Enhancement of cell adhesion and osteogenic differentiation in vitro

Amide‐linkage formed between ammonia plasma treated poly(D,L‐lactide acid) scaffolds and bio‐peptides: Enhancement of cell adhesion and osteogenic differentiation in vitro

Modulating protein adsorption onto hydroxyapatite particles using different amino acid treatments

Antibacterial burst-release from minimal Ag-containing plasma polymer coatings

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