This research analyzes how organizational ambidexterity (OA) affects sustainable performance in the Spanish wine industry, as well as the catalytic role of corporate social responsibility (CSR) practices and knowledge management infrastructure (KMI) on these variables. For this purpose, a theoretical model is elaborated based on the literature review carried out, which is contrasted following a quantitative approach by means of structural equation modeling (PLS‐SEM) with primary data collected from 202 Spanish wineries between September 2021 and January 2022. The research results show that there is a positive and significant relationship between OA and sustainable performance. Likewise, OA partially mediates the CSR‐sustainable performance and KMI‐sustainable performance relationship. Thus, the present research contributes to the literature in several ways. First, to the best of our knowledge, there are no previous studies that have analyzed the effect of OA on sustainable performance in the wine context, which represents an opportunity to advance scientific knowledge. Second, to our knowledge, there is no previous scientific production that has analyzed what may be the triggers of OA. Third, the proposed theoretical model has not been addressed previously, so that, through the research, a new way of understanding the improvement in the economic, social and environmental performance of wineries is formulated [EconLit Citations: L66, 03, M1].