The effect of immobilized RGD peptide in macroporous alginate scaffolds on TGFβ1-induced chondrogenesis of human mesenchymal stem cells

Cartilage Tissue Engineering Using Mesenchymal Stem Cells and 3D Chitosan Scaffolds – In vitro and in vivo Assays

“…The results agreed well with previous studies showing that initial cell adhesion was largely influenced of RGD in gelatin bound to chitosan (Dong et al, 2010). It was reported that immobilization of RGD peptide onto a scaffold enabled the adhesion of stem cells to the scaffold and inhibited the immediate matrix-induced cell aggregation (Re'em et al, 2010). It allowed better access for cells to nutrients, oxygen and chondrogenic inducer.…”

Culture of hESC‐derived pancreatic progenitors in alginate‐based scaffolds

“…In normal BM, laminin is tethered to the collagen network, and as the laminin added to the system in this study was not immobilized by conjugation to the matrix, it may not have been appropriately presented for proper receptor binding and signaling to occur. degree of substitution [54][55][56]. It is still possible that this degree of coupling results in a peptide density too low to induce significant cellular response for the system studied herein [57], and further studies may include alginates with a higher peptide density produced with methods other than carbodiimid chemistry.…”

Fast-Degradable Microbeads Encapsulating Human Umbilical Cord Stem Cells in Alginate for Muscle Tissue Engineering

“…61 Although soluble RGD peptide is related with cell death, 31,62 immobilized RGD peptide is important for cell adhesion, [63][64][65] migration, 19,66 and differentiation. 37,55 In the present study, when macropores were created within the matrix with the degradation of microbeads, cells were released into the macroporous matrix, thus allowing the RGDmodified AM to help enhance myogenic differentiation. Further studies are needed to investigate the effects of porosity and incorporation of RGD and other bioactive agents on in vivo muscle regeneration via the novel hUCMSCmicrobead-AM construct in an animal model.…”

“…37 Then, the hydrogels were incubated in the low-glucose DMEM buffer for 1 h to block the nonspecific binding. The samples were incubated overnight with primary antibodies (including MyoD, myogenin, ACTN, and MYH; 1:100) in the DMEM buffer containing 5% FBS and then washed with DMEM for three times.…”