The effect of injury upon the uptake of 3H-thymidine by guinea pig epidermis

Hell, Elizabeth; Cruickshank, C. N. D.

doi:10.1016/0014-4827(63)90162-9

Cited by 89 publications

(15 citation statements)

References 14 publications

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“…The intravital findings in the present study confirm those of various authors using other animal species (guinea pig, Hell and Cruickshank 1963;mouse, Bullough and Laurence 1960). Proliferation in those studies generally began 24 hours post-injury and peaked at 48 hours.…”

Section: Discussionsupporting

confidence: 95%

Temporal course of intravital and postmortem proliferation of epidermal cells after mechanical injury

Oehmichen

Cröpelin

1995

Int J Leg Med

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The temporal course of epidermal basal cell proliferation in the wound of the pinna of rats was studied using bromodeoxyuridine (BrdU) immunohistochemistry. Following incisional wounding, the animals were sacrificed at intervals ranging from 0 hours to 32 days. Two biopsies were taken from each animal, one intravitally and one postmortem after 24 hours storage at 8 degrees C. Specimens were incubated in a solution containing BrdU and embedded in paraffin. BrdU expression was demonstrated by a monoclonal antibody against BrdU. In both intravital and postmortem biopsies, the labelling indices increased significantly in the period from 32 to 60 hours post-injury. This suggests that DNS synthesis induced during life continues after death. Applied to forensic practice, the present findings point to the possibility of determining the vitality of a wound in postmortem tissue.

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Section: Discussionsupporting

confidence: 95%

Temporal course of intravital and postmortem proliferation of epidermal cells after mechanical injury

Oehmichen

Cröpelin

1995

Int J Leg Med

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“…However, clues may be found in a thorough investigation of wound healing at the neonatal stage, which possibly involves transitions of some aspects of wound healing. For example, when excisional wounds were made on the skin of neonatal rats, signs of thickening and inward spreading of the marginal epidermis were detected within 24 h after wounding, but the morphological change at such an early phase of wound healing was not accompanied by any stimulation of cell proliferation (unpublished results), consistent with previous findings on adult wounds (Devitt et al 1978;Hell and Cruickshank 1963). The present study has focused on an attempt to identify and characterize the subsets of epidermal cells that cover the open space of excisional wounds on the neonatal rat skin.…”

Section: Introductionsupporting

confidence: 88%

The subsets of keratinocytes responsible for covering open wounds in neonatal rat skin

Koizumi

Matsuzaki

Ihara

2004

Cell and Tissue Research

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Full-thickness excisional wounds were made on the dorsal skin of 1-day-old rats to elucidate from where the cells move into the defect and what kinds of cells they are. Immunohistochemical analyses of the wound sites revealed that the following two subsets of keratinocytes were the major contributors to reepithelialization: first, the cells at the forefront of the migrating epithelium, termed "leading edge cells," which expressed K14 keratin, known as basal cell-specific keratin, but not K6 or K10 keratins, so that they had probably moved from the basal cell layer; and, second, the cells tentatively termed "immature spinous cells," which expressed K14 and K6 but not K10, and formed an "ingrowth region" following the leading edge cells. These two kinds of cells moved to the open wound area, as a multilayered cell sheet. Fluorescent phalloidin staining experiments indicated that actin filaments became concentrated in the leading edge cells within 6 h postwounding (PW), whereas weak signals of actin filaments were detected in the immature spinous cells. Taken together, the present findings support the view that wound covering in neonatal rat skin is accomplished by a movement en masse of keratinocytes from the bottom half of the surrounding epidermis.

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“…In addition, by 24 h a "proliferative zone" of cells at the wound periphery had formed. The mitotically active cells in this zone resided in the basal cell layer and occasionally in the lowest spinous cell layer (Hell and Cruickshank, 1963; our unpublished observations), whereas cells in the upper layers were differentiating to restore the epidermal permeability barrier. Six days after injury the epidermis had almost healed.…”

Section: Wound Morphologiesmentioning

confidence: 72%

Wounding alters epidermal connexin expression and gap junction-mediated intercellular communication.

Goliger

Paul

1995

MBoC

180

181

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We show that connexin expression and in vivo patterns of communication were dramatically altered in response to epidermal wounding. Six hours after injury, Cx26 was up-regulated in the differentiated cells proximal to the wound, but was down-regulated in cells located at the wound edge. In contrast, Cx31.1 and Cx43 were down-regulated in cells both peripheral to and at the wounded edge. These patterns of altered connexin expression were detectable as early as 2 h after wounding and were most pronounced in 24-h old wounds. Increased expression of Cx26 was still evident in the hyperproliferative epidermis of 6-day old wounds. In vivo dye transfer experiments with Lucifer yellow and neurobiotin confirmed that junctional communication patterns were altered in ways consistent with changes in connexin expression. The data thus suggest that intercellular communication is intimately involved in regulating epidermal wound repair.

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The effect of injury upon the uptake of 3H-thymidine by guinea pig epidermis

Cited by 89 publications

References 14 publications

Temporal course of intravital and postmortem proliferation of epidermal cells after mechanical injury

Temporal course of intravital and postmortem proliferation of epidermal cells after mechanical injury

The subsets of keratinocytes responsible for covering open wounds in neonatal rat skin

Wounding alters epidermal connexin expression and gap junction-mediated intercellular communication.

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