The effect of inoculants and cellulase on the fermentation and microbiological composition of grass silage: II Microbiological changes in the silages

Abstract

Abstract. Four experimental silages were made into bunker silos by adding lactic acid bacteria and cellulase enzyme. The control silages were made without any additives and with AIV II solution (80 % formic acid, 2°/o orthophosphoric acid). The microbial compositions of the experimental silages were studied in five microbial groups; (1) total count of bacteria, (2) lactic acid bacteria, (3) Clostridia, (4) coliform bacteria and (5) fungi (yeasts and moulds).At the beginning of the fermentations enteric bacteria and lactic acid bacteria were increasing. Inoculation of the silage decreased the growth of enterobacteria compared with silages made with cellulase or without additives. This effect was further increased by using AIV II solution. Heterolactic fermentations started in the silages made with cellulase, inoculants and no additives, when initial reducing sugars, primarily glucose and fructose, were fermented. In good ensiling conditions only a few butyric acid forming Clostridia and small amounts of moulds and yests were found. Yeasts existed mostly in silages made with cellulase and with inoculation.

Influence of nitrogen fertilization on tropical-grass silage assessed by ensiling process monitoring using chemical and microbial community analyses

“…sugars and enzymes) available to lactic acid bacteria, or in herbage with an adequate amount of WSCs, lactic acid bacterial inoculants are used to ensure lactic acid‐dominant fermentation (Seale et al. 1986; Rauramaa et al. 1987; Selmer‐Olsen et al.…”

Effects of enzymes and nutrients in a bacterial inoculant on quality of timothy or alfalfa silage and dairy cow performance

“…Consequently, the minor effects of inoculants on cfu and silage quality observed in this study are consistent. Rauramaa et al (1987) …”

The impact of enzyme characteristics on corn stover fiber degradation and acid production during ensiled storage

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