The Effect of iNOS Inhibitors and Hyperbaric Oxygen Treatment in a Rat Model of Experimental Colitis

Erçin, Cemal Nuri; Yeşilova, Zeki; Korkmaz, Ahmet; Özcan, Ayhan; Öktenli, Çağatay; Uygun, Ahmet

doi:10.1007/s10620-008-0498-1

Cited by 16 publications

(10 citation statements)

References 40 publications

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“…The results suggest the development of liver necrosis and cholestasis, which is evident after 72 h; however, lipid peroxidation started after 1-2 weeks of BDL [32]. Our results are in agreement with Ercin et al [33], who reported that in BDL rats, the activity of the antioxidant systems diminished, which is responsible for the inhibition of the free oxygen radicals. The administration of albumin significantly increased the catalase activity and GSH level as well as reduced the MDA level and thus reduced reactive free radical induced oxidative damage, protected the tissues from highly reactive hydroxyl radicals and attenuated lipid peroxidation in the kidney of the BDL rats.…”

Section: Discussionsupporting

confidence: 92%

The effect of albumin administration on renal dysfunction after experimental surgical obstructive jaundice in male rats

Saad

Fathelbab

El-Saba

et al. 2016

Journal of Taibah University for Science

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The aim was to study the influence of albumin supplementation on the changes of the kidney function and structure in cirrhotic rats induced by common bile duct ligation (BDL). Twenty-four male albino rats weighing 200-250 g were divided into Group I: 6 rats underwent laparotomy alone, and the bile duct was only dissected from the surrounding tissue; Group II: 6 rats underwent a sham operation and received 2% albumin in their drinking water; Group III: 6 rats were subjected to bile duct ligation only; and Group IV: 6 rats were subjected to bile duct ligation and received a daily albumin 2% in drinking water. All rats were sacrificed after 4 weeks. We measured the liver and kidney functions and oxidative stress markers in the renal tissue and conducted a histological evaluation of the liver and kidney. The liver enzymes were decreased, but there was no significant difference in the bilirubin levels in group IV compared to group III. There was a significant elevation of serum creatinine in group III compared to group II, and serum creatinine was attenuated in group IV. The renal tissue catalase activity and reduced glutathione, as well as the nitric oxide levels, were significantly increased in group IV and were elevated in group III. Histologically, the livers of group IV showed degeneration and inflammatory cell infiltration with regeneration areas in which normal hepatocytes appeared. The kidneys of group IV showed recovery as well as areas of inflammatory cell infiltration. Some tubules appeared with normal epithelial lining. In conclusion, the results suggest that albumin partially improves the renal functions and structures after their disturbances as a result of BDL.

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Section: Discussionsupporting

confidence: 92%

The effect of albumin administration on renal dysfunction after experimental surgical obstructive jaundice in male rats

Saad

Fathelbab

El-Saba

et al. 2016

Journal of Taibah University for Science

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“…Two studies reported HBOT significantly increased glutathione peroxidase and superoxide dismutase levels [60,61]. HBOT also decreased nitric oxide [58,61,63] and nitric oxide synthase levels [63,64,66]. Finally, in one study, HBOT decreased prostaglandin E 2 levels [64].…”

Section: Resultsmentioning

confidence: 99%

Hyperbaric oxygen treatment for inflammatory bowel disease: a systematic review and analysis

Rossignol¹

2012

Med Gas Res

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BackgroundTraditionally, hyperbaric oxygen treatment (HBOT) has been used to treat a limited repertoire of disease, including decompression sickness and healing of problem wounds. However, some investigators have used HBOT to treat inflammatory bowel disease (IBD), including Crohn's disease and ulcerative colitis.MethodsComprehensive searches were conducted in 8 scientific databases through 2011 to identify publications using HBOT in IBD. Human studies and animal models were collated separately.ResultsThirteen studies of HBOT in Crohn's disease and 6 studies in ulcerative colitis were identified. In all studies, participants had severe disease refractory to standard medical treatments, including corticosteroids, immunomodulators and anti-inflammatory medications. In patients with Crohn's disease, 31/40 (78%) had clinical improvements with HBOT, while all 39 patients with ulcerative colitis improved. One study in Crohn's disease reported a significant decrease in proinflammatory cytokines (IL-1, IL-6 and TNF-alpha) and one study in ulcerative colitis reported a decrease in IL-6 with HBOT. Adverse events were minimal. Twelve publications reported using HBOT in animal models of experimentally-induced IBD, including several studies reporting decreased markers of inflammation or immune dysregulation, including TNF-alpha (3 studies), IL-1beta (2 studies), neopterin (1 study) and myeloperoxidase activity (5 studies). HBOT also decreased oxidative stress markers including malondialdehyde (3 studies) and plasma carbonyl content (2 studies), except for one study that reported increased plasma carbonyl content. Several studies reported HBOT lowered nitric oxide (3 studies) and nitric oxide synthase (3 studies) and one study reported a decrease in prostaglandin E2 levels. Four animal studies reported decreased edema or colonic tissue weight with HBOT, and 8 studies reported microscopic improvements on histopathological examination. Although most publications reported improvements with HBOT, some studies suffered from limitations, including possible publication and referral biases, the lack of a control group, the retrospective nature and a small number of participants.ConclusionsHBOT lowered markers of inflammation and oxidative stress and ameliorated IBD in both human and animal studies. Most treated patients were refractory to standard medical treatments. Additional studies are warranted to investigate the effects of HBOT on biomarkers of oxidative stress and inflammation as well as clinical outcomes in individuals with IBD.

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“…Sustained, high levels of NO production, especially when mediated by iNOS in the colon, play a role in the pathology of IBD [26–28]. Several synthetic iNOS inhibitors were effective in suppressing DSS-induced colitis symptoms in mice [26–28]. Marked elevated levels of PGE 2 and COX-2 were also observed during inflammation, including DSS-induced colonic injury [29].…”

Section: Discussionmentioning

confidence: 99%

Ethanol Extract ofAntrodia camphorataGrown on Germinated Brown Rice Suppresses Inflammatory Responses in Mice with Acute DSS-Induced Colitis

Park

2013

Evidence-Based Complementary and Alternative Medicine

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The anti-inflammatory activity of Antrodia camphorata (AC) grown on germinated brown rice (CBR) extract was evaluated in vitro and in vivo. CBR suppressed the release of nitric oxide (NO) and prostaglandin (PG) E2 from lipopolysaccharide-(LPS-)stimulated RAW264.7 cells. CBR inhibited the level of inducible nitric oxide synthase (iNOS) and cyclooxygenase-(COX-)2 proteins, and it activated p38-MAPK, extracellular signal-related kinases (ERK), and NF-κB in LPS-stimulated RAW264.7 macrophages. LPS-induced tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) mRNA expression was reduced in CBR-treated RAW264.7 cells. In concert with in vitro data, CBR suppressed the levels of dextran-sulfate-sodium-(DSS-)induced iNOS and COX-2 proteins in the colon tissue. CBR treatment inhibited activated p38-MAPK, ERK, and NF-κB proteins in the colon tissue of DSS-induced mice. TNF-α and IL-6 mRNA expression was reduced in DSS+CBR-treated mice. The disease activity index and histological scores were significantly lower in CBR-treated mice (500 mg/kg/day) than in DSS-treated mice (P < 0.05 versus DSS). This is the first report of anti-inflammatory activity of CBR in DSS-induced acute colitis. These results suggest that CBR is a promising, potential agent for preventing acute colitis through the inhibition of NF-κB signaling and its upstream signaling molecules, including MAPKs.

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The Effect of iNOS Inhibitors and Hyperbaric Oxygen Treatment in a Rat Model of Experimental Colitis

Abstract: Both HBO and AMG were significantly effective in preventing weight loss, reducing NO activities, and severity of colitis, when comparing HBO and AMG separately.

Cited by 16 publications

References 40 publications

The effect of albumin administration on renal dysfunction after experimental surgical obstructive jaundice in male rats

The effect of albumin administration on renal dysfunction after experimental surgical obstructive jaundice in male rats

Hyperbaric oxygen treatment for inflammatory bowel disease: a systematic review and analysis

Ethanol Extract ofAntrodia camphorataGrown on Germinated Brown Rice Suppresses Inflammatory Responses in Mice with Acute DSS-Induced Colitis

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