The effect of intracerebroventricular application of the caspase-3 inhibitor zDEVD-FMK on neurological outcome and neuronal cell death after global cerebral ischaemia due to cardiac arrest in rats

Teschendorf, Peter; Vogel, Peter; Wippel, Andreas; Krumnikl, J. J.; Spöhr, Fabian; Böttiger, Bernd W.; Popp, Erik

doi:10.1016/j.resuscitation.2008.02.010

Cited by 18 publications

(13 citation statements)

References 46 publications

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“…In general, preliminary study indicated the patients can benefit from induced hypothermia, but the optimal level of hypothermia and the best cooling methods at the beginning of treatment were still under investigation. A number of cerebral protections should be performed by comprehensive treatment, because of the complex mechanisms of brain injury after CA ( 4 ). Therefore, the cerebral function may be improved by developing more brain protection plans in cerebral resuscitation study.…”

Section: Introductionmentioning

confidence: 99%

Bone marrow mesenchymal stem cells repair the hippocampal neurons and increase the expression of IGF‑1 after cardiac arrest in rats

et al. 2017

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The present study aimed to investigate the beneficial effects and underlying mechanisms of bone marrow mesenchymal stem cells (BMSCs) on global ischemic hypoxic brain injury. Cells collected from the femurs and tibias of male Sprague Dawley rats were used to generate BMSCs following three culture passages. A rate model of cardiac arrest (CA) was induced by asphyxia. One hour following return of spontaneous circulation (ROSC), BMSCs were transplanted through injection into the tail vein. Neurological status was assessed using modified neurological severity score (mNSS) tests 1, 3 and 7 days following ROSC. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and immunohistochemical staining were used to detect insulin-like growth factor 1 (IGF-1) expression in the hippocampus. Furthermore, double-fluorescent labeling of green fluorescent protein (GFP) and IGF-1 was used to detect the IGF-1 expression in transplanted BMSCs. Serum levels of protein S100-B were examined using ELISA. GFP-labeled BMSCs were observed in the hippocampus at 1, 3 and 7 days post transplantation through fluorescent microscopy. BMSC transplantation resulted in reduced protein S100-B levels. The mNSS of the BMSC-treatment group was significantly reduced compared with that of the CA group. The RT-qPCR analysis and immunohistochemistry results demonstrated that BMSC treatment significantly increased IGF-1 expression in the hippocampus. In addition, the double-fluorescent labeling results demonstrated that transplanted BMSCs expressed IGF-1 in the hippocampus. The results of the present study suggest that BMSC treatment promotes the recovery of cerebral function following CA in rats possibly through the secretion of IGF-1.

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Section: Introductionmentioning

confidence: 99%

Bone marrow mesenchymal stem cells repair the hippocampal neurons and increase the expression of IGF‑1 after cardiac arrest in rats

et al. 2017

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“…On the other hand, it is the main terminal peptidase playing a critical role in apoptosis (Teschendorf et al, 2008). Additionally, caspase-3 is required for some typical hallmarks of apoptosis such as, chromatin condensation and DNA fragmentation in all cell types (Porter and Janicke, 1999).…”

Section: Discussionmentioning

confidence: 99%

Merit of quinacrine in the decrease of ingested sulfite-induced toxic action in rat brain

Kencebay

Derın

Özsoy

et al. 2013

Food and Chemical Toxicology

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“…25 Caspase-3 is the main terminal peptidase in apoptosis and plays a critical role in apoptosis induction. 26 Removal of caspase-3 from cell extracts abrogates apoptosis induction, and the addition of exogenous purified caspase-3 allows apoptosis to proceed. Caspase-3 has been referred to as the ''death protein''.…”

Section: Discussionmentioning

confidence: 99%

The effect of ischemic post-conditioning on hippocampal cell apoptosis following global brain ischemia in rats

Zhang

Wang

Zhou

et al. 2012

Journal of Clinical Neuroscience

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The effect of intracerebroventricular application of the caspase-3 inhibitor zDEVD-FMK on neurological outcome and neuronal cell death after global cerebral ischaemia due to cardiac arrest in rats

Cited by 18 publications

References 46 publications

Bone marrow mesenchymal stem cells repair the hippocampal neurons and increase the expression of IGF‑1 after cardiac arrest in rats

Bone marrow mesenchymal stem cells repair the hippocampal neurons and increase the expression of IGF‑1 after cardiac arrest in rats

Merit of quinacrine in the decrease of ingested sulfite-induced toxic action in rat brain

The effect of ischemic post-conditioning on hippocampal cell apoptosis following global brain ischemia in rats

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