The effect of intranasally administered TLR3 agonist larifan on metabolic profile of microglial cells in rat with C6 glioma

Hurmach, Yevheniia; Rudyk, Mariia; Svyatetska, V.; Senchylo, N.; Skachkova, O.; Pjanova, Dace; Vaivode, Kristine; Skivka, Larysa

doi:10.15407/ubj90.06.110

Cited by 3 publications

(2 citation statements)

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“…Nitrite level examination was conducted to evaluate NO release into the conditioned media of phagocytic cells as described previously . Briefly, after the treatment of cells with nanoformulations, the conditioned media were collected, and the nitrite concentration in each sample was assayed by the Griess assay.…”

Section: Methodsmentioning

confidence: 99%

“…Nitrite level examination was conducted to evaluate NO release into the conditioned media of phagocytic cells as described previously. 54 Briefly, after the treatment of cells with nanoformulations, the conditioned media were collected, and the nitrite concentration in each sample was assayed by the Griess assay. Equal volumes of 2% sulfanilamide in 10% phosphoric acid and 0.2% N-(1-naphthyl)ethylenediamine dihydrochloride were mixed to prepare the Griess reagent (GR).…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

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C₆₀ Fullerene Governs Doxorubicin Effect on Metabolic Profile of Rat Microglial Cells In Vitro

et al. 2020

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Background: C 60 fullerenes and their derivatives are actively investigated for the use in neuroscience. Applications of these nanoscale materials require the examination of their interaction with different neural cells, especially with microglia, because these cells, like other tissue resident phagocytes, are the earliest and most sensitive responders to nanoparticles. The aim of this study was to investigate the effect of C 60 fullerene and its nanocomplex with doxorubicin (Dox) on the metabolic profile of brain-resident phagocytesmicrogliain vitro. Methods: Resting microglial cells from adult male Wistar rats were used in experiments. Potential C 60 fullerene targets in microglial cells were studied by computer simulation. Microglia oxidative metabolism and phagocytic activity were examined by flow cytometry. Griess reaction and arginase activity colorimetric assay were used to explore arginine metabolism. Results: C 60 fullerene when used alone did not influence microglia oxidative metabolism and phagocytic activity but shifted arginine metabolism toward the decrease of NO generation. Complexation of C 60 fullerene with Dox (C 60 −Dox) potentiated the ability of the latter to stimulate NO generation. Conclusion: The capability of C 60 fullerenes used alone to cause anti-inflammatory shift of microglia arginine metabolism makes them a promising agent for the correction of neuroinflammatory processes involved in neurodegeneration. The potentiating action of C 60 fullerene on the immunomodulatory effect of Dox allows us to consider the C 60 molecule as an attractive vehicle for this antitumor agent.

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Section: Methodsmentioning

confidence: 99%

Section: ■ Experimental Sectionmentioning

confidence: 99%

C₆₀ Fullerene Governs Doxorubicin Effect on Metabolic Profile of Rat Microglial Cells In Vitro

et al. 2020

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POLARIZED ACTIVATION OF HUMAN PERIPHERAL BLOOD PHAGOCYTES BY BACTERIOPHAGE–DERIVED DOUBLESTRANDED RNA (LARIFAN) in vitro

Dovhyi

2023

Biotechnol. acta

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Aim. This study aimed to examine the effect of Larifan on metabolic characteristics of human blood monocytes and granulocytes in vitro. Methods. Four healthy adult men aged 21–26 years were recruited to participate in the study as blood donors. The metabolic profile of human blood monocytes and granulocytes was evaluated by phagocytic activity, reactive oxygen species production, nitric oxide generation, and arginase activity. Phagocytosis of FITC-labeled inactivated Staphylococcus aureus and reactive oxygen species generation were estimated by flow cytometry. Arginase activity was assessed in cell lysates, and nitric oxide generation in supernatants was examined using the Griess reaction. Results. Phagocytic index and reactive oxygen species generation were found to be lower in both human blood monocytes and granulocytes treated with Larifan. The drug caused a dose-dependent increase in nitric oxide production, as well as a decrease in the arginase activity of blood monocytes. Conclusions. Our results indicate the ability of Larifan to reinforce the antiviral properties of resting phagocytes along with containment of oxidative stress development.

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Bacteriophage–derived double-stranded RNA (larifan) exerts variable effects on human blood monocytes depending on age and sex of donors

Dovhyi,

Rudyk,

Serhiichuk

et al. 2024

Ukr.Biochem.J.

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To date, great attention is paid to sex and age differences in the therapeutic effectiveness of drugs, including those that impact the immune system. Bacteriophage-derived dsRNA is the main component of the medicinal product Larifan, which exhibits interferonogenic activity. This study aimed to estimate the effect of Larifan on the activation status of human peripheral blood monocytes collected from donors of different ages and sex. Blood samples were obtained from the healthy volunteers, divided into 4 groups: young men and young women aged from 20 to 39 years, aged men and aged women from 54 to 69 years old. EDTA-anticoagulated blood samples were exposed to 200 μg/ml Larifan for 30 min, cells were washed and treated to study phagocytic index, ROS generation and expression of phenotypic markers. Only live monocytes selected by flow cytometry were included in the analysis. It was shown that monocytes from young as well as from aged females turned out to be quite inert to the treatment with Larifan. Monocytes from young males after the treatment demonstrated a minor decrease in phagocytic activity and significant down-regulation of ROS generation. Monocytes from aged adults showed clear sex-based differences in the basal cell phenotype. Thus, compared to monocytes from women, the monocytes from men over 50 after the treatment with Larifan showed decreased phagocytic activity and CD86 expression along with increased CD206 expression. Taken together, these results indicate the need for further studies of Larifan focused on developing personalized treatment depending on the age and sex of an individual. Keywords: double-stranded RNA, Larifan, monocytes, phagocytosis, reactive oxygen species, sex and age differences

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The effect of intranasally administered TLR3 agonist larifan on metabolic profile of microglial cells in rat with C6 glioma

Cited by 3 publications

References 44 publications

C₆₀ Fullerene Governs Doxorubicin Effect on Metabolic Profile of Rat Microglial Cells In Vitro

C₆₀ Fullerene Governs Doxorubicin Effect on Metabolic Profile of Rat Microglial Cells In Vitro

POLARIZED ACTIVATION OF HUMAN PERIPHERAL BLOOD PHAGOCYTES BY BACTERIOPHAGE–DERIVED DOUBLESTRANDED RNA (LARIFAN) in vitro

Bacteriophage–derived double-stranded RNA (larifan) exerts variable effects on human blood monocytes depending on age and sex of donors

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The effect of intranasally administered TLR3 agonist larifan on metabolic profile of microglial cells in rat with C6 glioma

Cited by 3 publications

References 44 publications

C60 Fullerene Governs Doxorubicin Effect on Metabolic Profile of Rat Microglial Cells In Vitro

C60 Fullerene Governs Doxorubicin Effect on Metabolic Profile of Rat Microglial Cells In Vitro

POLARIZED ACTIVATION OF HUMAN PERIPHERAL BLOOD PHAGOCYTES BY BACTERIOPHAGE–DERIVED DOUBLESTRANDED RNA (LARIFAN) in vitro

Bacteriophage–derived double-stranded RNA (larifan) exerts variable effects on human blood monocytes depending on age and sex of donors

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C₆₀ Fullerene Governs Doxorubicin Effect on Metabolic Profile of Rat Microglial Cells In Vitro

C₆₀ Fullerene Governs Doxorubicin Effect on Metabolic Profile of Rat Microglial Cells In Vitro