The effect of intravenous human adipose–derived stem cells (hADSC) on transforming growth factor β1 (TGF-β1), collagen type 1, and kidney histopathological features in the unilateral ureteropelvic junction obstruction model of wistar rats

Siregar, Safendra; Noegroho, Bambang Sasongko; Karim, Muhammad Ilhamul

doi:10.5152/tud.2020.20024

Cited by 3 publications

(2 citation statements)

References 22 publications

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“…The mechanism may be that the high expression of transforming growth factor-β1 (TGF-β1) in the obstructed kidney promotes the accumulation of collagen [ 75 ]. In the UPJO Model of Wistar Rats, it is found that the level of TGF-β gradually decreased and the degree of renal fibrosis is less in the experimental group after tail vein injection of ADSCs [ 76 ]. The main mechanism is that ADSCs can inhibit the expression of pro-fibrotic genes such as COL 1A 1, TGF-β1, and connective tissue growth factor (CTGF) [ 77 ].…”

Section: Adscs In Urinary Tract Repairmentioning

confidence: 99%

Adipose stem cells in tissue regeneration and repair: From bench to bedside

Dong,

Li,

Leng

et al. 2023

Regenerative Therapy

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Section: Adscs In Urinary Tract Repairmentioning

confidence: 99%

Adipose stem cells in tissue regeneration and repair: From bench to bedside

Dong,

Li,

Leng

et al. 2023

Regenerative Therapy

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“…14,15 TGFβ1 is a profibrotic cytokine which stimulates mesenchymal cell proliferation and extracellular matrix deposition. [16][17][18] In organ culture, exogenous TGFβ postpones the ureterovesical smooth muscle proliferation and affects the muscular structure. 19 Fibroblast growth factor (FGF) is a group of growth factors that plays role in a kidney and lower urinary tract development, for instance, nephron progenitor differentiation in nephrons and development of ureteric epithelium.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of PGP 9.5, NGFR, TGFβ1, FGFR1, MMP-2, AT2R2, SHH, and TUNEL in Primary Obstructive Megaureter Tissue

Junga

Siņicins

Pētersons

et al. 2021

J Histochem Cytochem.

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Primary obstructive megaureter (POM) morphogenesis is not fully known. The aim of the study was to evaluate the appearance of different factors that might take part in the pathogenesis of POM. Megaureter tissues of 14 children were stained with hematoxylin and eosin as well as with immunohistochemistry for protein gene product 9.5, nerve growth factor receptor, transforming growth factor beta 1 (TGFβ1), fibroblast growth factor receptor 1 (FGFR1), matrix metalloproteinase 2 (MMP-2), angiotensin 2 receptor type 2, and sonic hedgehog (SHH) protein. Apoptosis was detected by terminal dUTP nick-end labeling reaction. POM tissues revealed transitional epithelium with scattered vacuolization, submucosa with inflammatory cells, and focally vacuolized and chaotically organized muscle layers. Apoptosis, appearance of MMP-2, FGFR1, and SHH prevailed, but TGFβ1 positive cell number was lower in patients. Correlation between MMP-2 in epithelium and endothelium, FGFR1 and MMP-2 in epithelium, and TGFβ1 in epithelium and connective tissue in patients was detected. POM morphopathogenesis involves an apoptotic cell death of epithelium and smooth muscle as well as tissue degradation in epithelium and connective tissue of the ureter wall. The decrease of tissue growth through diminished TGFβ1 expression and stimulation of FGFR1 and MMP-2 suggests a disbalance of tissue remodelation in the megaureter wall:

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The effect of adipose-derived stem cells (ADSC) treatment on kidney histopathological appearance on the Wistar rat models with grade five kidney trauma

Agil,

Romdam,

Atik

et al. 2024

Innovative Surgical Sciences

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Objectives Kidney trauma is the most common urological trauma. Technological advances have made conservative management possible for almost all kidney trauma. However, grade five kidney trauma needs to be carefully examined due to its various complications, especially late complications that often delayed in recognition thus forming irreversible morbidity, with the most common late complication is kidney damage due to ischemic and fibrotic process. This study aims to confirm the effect of Adipose-Derived Stem Cells (ADSC) on the prevention of fibrosis in grade five kidney trauma using Wistar rat models, where the fibrosis process will be measured with histopathological examination which had features of glomerular sclerosis, tubular atrophy, and interstitial fibrosis in kidney tissue, then followed by histopathological scoring and total renal score. Methods A total of 22 adult rats were divided into five groups: one healthy control group, two trauma groups without ADSC, and two others trauma groups with ADSC. Two different treatment times were set: two weeks and four weeks after treatment. The data were tested for normality (Shapiro-Wilk test), while differences between groups were assessed using one-way ANOVA or Kruskal-Wallis test if the distribution was not normal. Results For the result of total renal score, statistical analysis reveal a significant difference in the total renal score in the kidney trauma with ADSC group compared with kidney trauma without ADSC group in fourth week of observation (p=0.001). Conclusions These findings highlighted ADSC capability to prevent fibrosis caused by grade five kidney trauma on the Wistar rat models, as proven by significantly reduced histopathological grading on fibrosis.

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The effect of intravenous human adipose–derived stem cells (hADSC) on transforming growth factor β1 (TGF-β1), collagen type 1, and kidney histopathological features in the unilateral ureteropelvic junction obstruction model of wistar rats

Abstract: I n d e x e d i n P u b M e d , W e b o f S c i e n c e a n d S c o p u s Yasemin Yuyucu Karabulut, et al.; The role of COL6A1 and PD-1 expressions in renal cell carcinoma.

Cited by 3 publications

References 22 publications

Adipose stem cells in tissue regeneration and repair: From bench to bedside

Adipose stem cells in tissue regeneration and repair: From bench to bedside

Evaluation of PGP 9.5, NGFR, TGFβ1, FGFR1, MMP-2, AT2R2, SHH, and TUNEL in Primary Obstructive Megaureter Tissue

The effect of adipose-derived stem cells (ADSC) treatment on kidney histopathological appearance on the Wistar rat models with grade five kidney trauma

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