The Effect of iPS-Derived Neural Progenitors Seeded on Laminin-Coated pHEMA-MOETACl Hydrogel with Dual Porosity in a Rat Model of Chronic Spinal Cord Injury

Růžička, J; Romanyuk, Nataliya; Jiráková, Klára; Hejčl, Aleš; Janoušková, Olga; Machova, Lucia Urdzikova; Bochin, Marcel; Přádný, Martin; Vargová, Lýdia; Jendelová, Pavla

doi:10.1177/0963689718823705

Cited by 37 publications

(31 citation statements)

References 47 publications

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“…Overall, from a morphological point of view, NSCs are able to differentiate into neurons, astrocytes, and oligodendrocytes promoting axonal regrowth, remyelination and regeneration of the CST [145,150,165]. In addition, NSCs grafts showed consistently the capacity of filling the lesion cavity, reducing the glial scar and a high chance of survive after transplantation [144,166]. Physiologically, Kumamaru et al (2018) showed that differentiated cells from NSCs form new synapses within the host spinal cord below the lesion.…”

Section: Stem Cells In Sci: Past Present and Futurementioning

confidence: 99%

“…Finally, and particularly important, the vast majority of the studies transplanted human-derived NSCs into animal models, which imposes the use of immunosuppressant drugs in combination with the cells. From the immunological point of view, several studies showed that when exogenous NSCs are combined with immunosuppressant drugs they can successfully integrate in the rat/mouse spinal cord without immune rejection [27,166,167]. However, data from clinical trials have shown that when the immunosuppressant therapy is over, patients experience complications related with immune rejection of the new tissue (please see Section 2.4) [168].…”

Section: Stem Cells In Sci: Past Present and Futurementioning

confidence: 99%

“…Using a laminin-coated hydrogel with dual porosity, iPSC-NPs were seeded and further transplanted into a rat model of chronic SCI. The cells survived over time and promoted the growth of host axons, astrocytes, and blood vessels; however, no locomotor recovery was observed [166].…”

Section: Stem Cells In Sci: Past Present and Futurementioning

confidence: 99%

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Filling the Gap: Neural Stem Cells as A Promising Therapy for Spinal Cord Injury

et al. 2019

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Spinal cord injury (SCI) can lead to severe motor, sensory and social impairments having a huge impact on patients’ lives. The complex and time-dependent SCI pathophysiology has been hampering the development of novel and effective therapies. Current treatment options include surgical interventions, to stabilize and decompress the spinal cord, and rehabilitative care, without providing a cure for these patients. Novel therapies have been developed targeting different stages during trauma. Among them, cell-based therapies hold great potential for tissue regeneration after injury. Neural stem cells (NSCs), which are multipotent cells with inherent differentiation capabilities committed to the neuronal lineage, are especially relevant to promote and reestablish the damaged neuronal spinal tracts. Several studies demonstrate the regenerative effects of NSCs in SCI after transplantation by providing neurotrophic support and restoring synaptic connectivity. Therefore, human clinical trials have already been launched to assess safety in SCI patients. Here, we review NSC-based experimental studies in a SCI context and how are they currently being translated into human clinical trials.

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Section: Stem Cells In Sci: Past Present and Futurementioning

confidence: 99%

Section: Stem Cells In Sci: Past Present and Futurementioning

confidence: 99%

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Filling the Gap: Neural Stem Cells as A Promising Therapy for Spinal Cord Injury

et al. 2019

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“…Study of the cell-matrix interactions and the ECM composition effects on proliferation and differentiation, can contribute to the nerve repair and neural tissue engineering (Ruzicka et al 2019;Wu et al 2017;Zhang et al 2018a, b). Modifying alginate hydrogel with integrinbinding ligands such as LXW64 and LXY30 resulted in inducing differentiation of NPCs to oligodendrocytes (Wen et al 2019).…”

Section: Cell-ecm Interactionsmentioning

confidence: 99%

Nanomaterial integration into the scaffolding materials for nerve tissue engineering: a review

Arzaghi

Adel

Jafari

et al. 2020

Reviews in the Neurosciences

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The nervous system, which consists of a complex network of millions of neurons, is one of the most highly intricate systems in the body. This complex network is responsible for the physiological and cognitive functions of the human body. Following injuries or degenerative diseases, damage to the nervous system is overwhelming because of its complexity and its limited regeneration capacity. However, neural tissue engineering currently has some capacities for repairing nerve deficits and promoting neural regeneration, with more developments in the future. Nevertheless, controlling the guidance of stem cell proliferation and differentiation is a challenging step towards this goal. Nanomaterials have the potential for the guidance of the stem cells towards the neural lineage which can overcome the pitfalls of the classical methods since they provide a unique microenvironment that facilitates cell–matrix and cell–cell interaction, and they can manipulate the cell signaling mechanisms to control stem cells’ fate. In this article, the suitable cell sources and microenvironment cues for neuronal tissue engineering were examined. Afterward, the nanomaterials that impact stem cell proliferation and differentiation towards neuronal lineage were reviewed.

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“…Typically, the role of the glial barrier and reactive astrocytes is considered a negative one despite a known decisive role in maintaining the tissue structural integrity and the process of neuroregeneration during an early post-SCI period [111,112]. Studies with biomaterial-supported MSCs transplantation after SCI provide evidence to reduced glial scar and astrocyte activation both in acute [113], subacute [114], and chronic periods following SCI [115]. The decreased expression level of GFAP and less that of proteoglycans are estimated in the damaged region, which means that the glial barrier is reduced and axonal growth results [116,117].…”

Section: Communication Between Astrocytes and Mscsmentioning

confidence: 99%

Biomaterial-supported MSCs transplantation enhances cell-cell communication for spinal cord injury

Wang

yang

2020

Preprint

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The spinal cord is part of the central nervous system (CNS) and serves to connect the brain to the peripheral nervous system and peripheral tissues. The cell types that primarily comprise the spinal cord are neurons and several categories of glia, including astrocytes, oligodendrocytes, and microglia. Ependymal cells and small populations of endogenous stem cells, such as oligodendrocyte progenitor cells, also reside in the spinal cord [1]. Neurons are interconnected in circuits; those that process cutaneous sensory input are mainly located in the dorsal spinal cord, while those involved in proprioception and motor control are predominately located in the ventral spinal cord [2]. Due to the importance of the spinal cord, neurodegenerative disorders and traumatic injuries affecting the spinal cord will lead to motor deficits and loss of sensory inputs. Spinal cord injury (SCI), resulting in paraplegia and tetraplegia as a result of deleterious interconnected mechanisms encompassed by the primary and secondary injury, represents a heterogeneously behavioral and cognitive deficit that remains incurable. Following SCI, various barriers containing the neuroinflammation, neural tissue defect (neurons, microglia, astrocytes, and oligodendrocytes), cavity formation, loss of neuronal circuitry and function must be overcame[3]. Notably, the pro -inflammatory and anti-inflammatory effect s of cell-cell communication networks play critical roles in homeostatic, driving the pathophysiologic and consequent cognitive outcomes. In the spinal cord, astrocytes, oligodendrocytes and microglia are involved in not only development but also pathology. Glial cells play dual roles (negative vs. positive effects) in these processes. After SCI, detrimental effects usually dominate and significantly retard functional recovery, and curbing these effects is critical for promoting neurological improvement. Indeed, residential innate immune cells (microglia and astrocytes) and infiltrating leukocytes (macrophages and neutrophils), activated by SCI, give rise to full-blown inflammatory cascades. These inflammatory cells release neurotoxins (proinflammatory cytokines and chemokines, free radicals, excitotoxic amino acids, nitric oxide (NO)), all of which partake in axonal and neuronal deficit[4]. Given the various multifaceted obstacles in SCI treatment, a combinatorial therapy of cell transplantation and biomaterial implantation may be addressed in detail here. For the sake of preserving damaged tissue integrity and providing physical support and trophic supply for axon regeneration, MSCs transplantation has come to the front stage in therapy for SCI with the constant progress of stem cell engineering [5]. MSCs transplantation promotes scaffold integration and regenerative growth potential. Integrating into the implanted scaffold, MSCs influences implant integration by improving the healing process[6]. Conversely, biomaterial scaffolds offer MSCs with a sheltered microenvironment from the surrounding pathological changes, in addition to bridging connection spinal cord stump and offering physical and directional support for axonal regeneration. Besides, Biomaterial scaffolds mimic the extracellular matrix to suppress immune responses. Here, we review the advances in combinatorial biomaterial scaffolds and MSCs transplantation approach that targets certain aspects of various intercellular communications in the pathologic process following SCI. Finally, the challenges of biomaterial-supported MSCs transplantation and its future direction for neuronal regeneration will be presented.

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The Effect of iPS-Derived Neural Progenitors Seeded on Laminin-Coated pHEMA-MOETACl Hydrogel with Dual Porosity in a Rat Model of Chronic Spinal Cord Injury

Cited by 37 publications

References 47 publications

Filling the Gap: Neural Stem Cells as A Promising Therapy for Spinal Cord Injury

Filling the Gap: Neural Stem Cells as A Promising Therapy for Spinal Cord Injury

Nanomaterial integration into the scaffolding materials for nerve tissue engineering: a review

Biomaterial-supported MSCs transplantation enhances cell-cell communication for spinal cord injury

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