The Effect of Isotoma longiflora Leaves Extract to The Cornea Neovascularization of Wistar Rats Chemical Trauma Model

Abstract

Eye chemical trauma was an eye trauma caused by a substance with a pH<7 (acid) and pH>7 (alkali). Chemical trauma can be caused by pesticides. In Indonesia, 78.9% of farmers had eye complaints due to pesticides exposure. The occurrence of alkali chemical trauma of eye could cause cornea neovascularization (CNV). Alkali chemical trauma caused intense inflammation resulting a vascularization of the cornea that was supposed to be avascular. The purpose of this study was to find out the effect of Isotoma longiflora leaves extract the cornea neovascularization of wistar rats chemical trauma model. This research used true experimental design method with post-test only controlled group design. The sample of this study were 30 male wistar rats (Rattus norvegicus) divided into six groups, 1 positive control group (dexamethasone 0.1%), 1 negative control group (DMSO 0,1%), and 4 treatment groups of Isotoma longiflora leaves extract (0.25 mg/ml, 0.5 mg/ml, 1 mg/ml, 2 mg/ml). The result of Shapiro-Wilk and Levene's test showed that p>0.05 that means the data was normally distributed and has the same variant. One Way ANOVA test results obtained significant differences between groups on the 7th day (p=0.001). The result of Post Hoc LSD test showed that group P3 and P4 were significantly different to negative control, P1 and P2 groups. P3 and P4 groups with 1 mg/ml and 2 mg/ml Isotoma longiflora leaves extract could inhibit cornea neovascularization.

The potency of kitolod leaves (Hipobroma longiflora) as traditional medicine for conjunctivitis during the COVID-19 pandemic: a brief review

“…Yulianto 24 Decoction of kitolod flowers was effective in inhibiting the growth of S. aureus, but it was not better than chloramphenicol Arsyad et al, 25 Kitolod leaves could inhibit the cornea neovascularization after 7 days in the eyes that had chemical trauma due to pesticide the Rotraz© 200EC.…”

“…Kitolod (H. longiflora) contains several bioactive compounds that act as antibacterials, anti-inflammation, analgesic, and antifungal. 25,36,37 All parts of this plant contain the same bioactive substances, but the most frequently found bioactive substances are in the leaves of the kitolod plant. 9 Bioactive substances are secondary metabolites that have biologically active characteristics.…”

Kandungan Flavonoid dan Aktivitas Antioksidan Kitolod (Hippobroma longiflora L.) pada Ketinggian Tempat Tumbuh Berbeda

“…Sebagian masyarakat Indonesia telah memanfaatkan tumbuhan kitolod sebagai obat tradisional yang dipercaya mampu mengatasi berbagai penyakit seperti katarak, mata minus, asma, sifilis, bronkhitis, radang tenggorokan, sakit gigi, dan menyembuhkan luka. Di Cina, tumbuhan ini juga dimanfaatkan untuk mengobati kanker, gigitan ular, dan sebagai anestesi lokal (Arsyad et al, 2020). Fazil et al (2017) menyebutkan bahwa ekstrak kitolod bersifat sebagai antiinflamasi, antikanker, antidiabetes, antifungi, antibakteri, antineoplastik, dan analgetik.…”