The effect of ketamine on the lipopolysaccharide-induced inflammation in in vitro culture of HUVEC

Asmoro, Aswoco Andyk; Rakhmatullah, Ramacandra; Puspitasari, Scarpia; Tarimah, Khairunnisai; Saleh, Siti Chasnak; Widodo, M. Aris; Widjajanto, Edi

doi:10.1016/s2222-1808(15)60952-5

Cited by 4 publications

(4 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A recent systematic review and meta-analysis have revealed that intraoperative administration of KET could attenuate the early postoperative IL-6 inflammatory reaction (Dale et al, 2012). Besides, it was reported that the levels of inflammatory cytokines including IL-6 and TNF-α were both downregulated after treatment of KET in all observation times (Asmoro et al, 2015). In the present study, we found that application of S-KET significantly reversed the CCl 4 -induced increased TNF-α, IL-1β, and IL-6 in dose-dependent manners, suggesting that S-KET inhibited CCl 4 -induced inflammatory response.…”

Section: Discussionmentioning

confidence: 99%

S-ketamine alleviates carbon tetrachloride-induced hepatic injury and oxidative stress by targeting the Nrf2/HO-1 signaling pathway

Wang

et al. 2021

Can. J. Physiol. Pharmacol.

View full text Add to dashboard Cite

The aim of the present study was to investigate the protective effect of S-ketamine (S-KET) against carbon tetrachloride (CCl4)-induced liver damage and oxidative stress, as well as to elucidate the related underlying mechanisms. Blood was collected to measure biochemical parameters (ALT, AST, ALP, TB and γ-GT) and the liver was harvested for histopathological analysis of enzymes related to the antioxidant response (MDA, SOD, GSH, and GSH-PX). Liver cell apoptosis was evaluated using the TUNEL assay. In addition, the expression levels of apoptosis-related proteins and the Nrf2/HO-1 signaling pathway were detected by western blot analysis to explore potential mechanisms. S-KET protected the liver from CCl4-induced damage. The changes to the liver biochemical parameters (increased ALT, AST, ALP, TB, and γ-GT) and oxidative stress-related indicators (increased MDA; depleted SOD, GSH, and GSH-PX) induced by CCl4 were inhibited by S-KET. S-KET also inhibited CCl4-induced cell apoptosis, the changes in expression of related proteins, and blocked CCl4-induced liver injury and oxidative stress via activation of the Nrf2/HO-1 signaling pathway. S-KET effectively protected the liver by inhibition of CCl4-induced damage via up-regulation the Nrf2/HO-1 signaling pathway.

show abstract

Section: Discussionmentioning

confidence: 99%

S-ketamine alleviates carbon tetrachloride-induced hepatic injury and oxidative stress by targeting the Nrf2/HO-1 signaling pathway

Wang

et al. 2021

Can. J. Physiol. Pharmacol.

View full text Add to dashboard Cite

show abstract

“…of cytokines production like TNF-α caused by ketamine may prevent apoptosis in sepsis, but further research is still needed. In vitro studies showed that ketamine reduce intracellular calcium elevations and caspase-3 in HUVEC cultured of sepsis model (Asmoro et al, 2015). The inflammatory response is a physiological and necessary phenomenon which is an active actor of homeostasis.…”

Section: Dmentioning

confidence: 99%

Ketamine Improves Sepsis-induced Immunosuppression by Attenuating Intracellular Calcium Elevation in Rat Peripheral Mononuclear Cells

Qodariyah¹,

Waafi

Suhardi

et al. 2018

RJLS

Self Cite

View full text Add to dashboard Cite

Immune response in sepsis divided into two phases, hyperinflammation (cytokine storm) and hypo-inflammation (immunosuppression). The mechanism of immunosuppression appears to involve apoptosis of immune cells, particularly mononuclear cells and it predisposes to secondary infection that is believed as the predominant driving force for mortality. We aimed to determine the effect of ketamine on mononuclear cells count related to intracellular calcium contained within these cells during the immunosuppression phase of sepsis. This in vivo study was performed in Rattus Novergicus with Fecal Induced Perionitis (FIP) procedure to induce polymicrobial sepsis. Rats were treated with each respective dose of ketamine (2.5, 5, and 10 mg/kg) 1 hour after sepsis induction. Murine Sepsis Score was measured at 1 and 24 hours post-FIP. After 24 hours, animals were sacrificed, and the percentage of intracellular calcium inside CD4, and CD8 T cells, B cells and monocytes, along with these cells counts were determined with flowcytometry. There is significant elevation of intracellular calcium in CD4 T-cells, B-cells and monocytes after FIP-induction and ketamine treatment suppressed this FIPinduced elevation. Measurement of mononuclear cells count showed a relevant result, in which FIP induced mononuclear cells massive loss and ketamine could inhibit the loss. MSS data showed ketamine 5 mg/kg could improve 24 hours MSS with 100% survivability. These findings suggest that ketamine have an inhibitory effect in mononuclearcells apoptosis mechanism through attenuating intracellular calcium elevation in polymicrobial sepsis. These inhibitory effects of ketamine might correlate with a better survival and clinical outcome.

show abstract

“…4 The annual cases of sepsis in the ICU RSUD Dr. Saiful Anwar, Indonesia, reached 168 patients, and 78 (46.4%) of them died. 5 Asmoro AA, et al ketamine effect on Kupffer cell in sepsis www.apicareonline.com 446 Open access attribute (CC BY-NC 4.0)…”

Section: Introductionmentioning

confidence: 99%

The effect of ketamine on Kupffer cell count in Wistar rat (Rattus norvegicus) model of sepsis

Asmoro¹,

Jaya²,

Wahyudi³

et al. 2022

Anaesth. pain intensive care

View full text Add to dashboard Cite

Background: Sepsis is a serious health problem and is associated with life-threatening complications, such as liver dysfunction, renal failure as well as cardiorespiratory disease. Kupffer cells are immune cells that contribute to the pathogenesis of liver dysfunction. This study aims to determine the effect of ketamine on the number of Kupffer cells in experimental animal models of sepsis. Methodology: This experimental study used fecal-induced peritonitis (FIP) to create a sepsis model in rats (Rattus norvegicus). Thirty rats were divided into six equal groups, namely the negative control group, which was not treated with FIP (Group K−), the positive control group or the sepsis model with FIP treatment (Group K+), and the treatment group; the sepsis model rats (FIP treatment) given 5 mg of ketamine/kg intraperitoneally once at 0 h (Group A), at 3 h (Group B), 5 h (Group C) after induction of sepsis with FIP, and every 2 h after induction of sepsis with FIP for four hours (Group D). Kupffer cells were counted six hours after FIP induction using a hematology analyzer. Statistical test was carried out using the One-Way ANOVA test using SPSS 18.0 software. Results: The number of Kupffer cells in sepsis groups (K+) was significantly higher (22.60 cell/ml) than in the negative control group (K-) (12.68 cell/ml) (P= 0.01). The administration of ketamine in the sepsis model group significantly decreased the number of Kupffer cells close to normal. The number of Kupffer cells was 12.56 cells/ml in Group A, 12.92 cells/ml in Group B, 9.75 cells/ml in Group C, and 8.50 cells/ml in Group D. Conclusion: The administration of ketamine decreased the number of Kupffer cells in the rat model of sepsis induced by FIP to close to the normal group. Abbreviations: APP - acute-phase protein; FIP - fecal induced peritonitis; ICU - Intensive Care Unit; IL – interleukin; KCs - Kupffer cells; ROS - reactive oxygen species; NO - nitric oxide; SIRS - systemic inflammatory response syndrome; TNF-α - tumor necrosis factor-alpha. Keywords: Immunomodulator; Ketamine; Kupffer cell; Sepsis Citation: Asmoro AA, Jaya W, Wahyudi AN, Laksono RM. The effect of ketamine on Kupffer cell count in Wistar rat (Rattus norvegicus) model of sepsis. Anaesth. pain intensive care 2022;26(4): 445-449. DOI: 10.35975/apic.v26i4.1946 Received: April 20, 2022; Reviewed: July 13, 2022; Accepted: July 13, 2022

show abstract

The effect of ketamine on the lipopolysaccharide-induced inflammation in in vitro culture of HUVEC

Cited by 4 publications

References 18 publications

S-ketamine alleviates carbon tetrachloride-induced hepatic injury and oxidative stress by targeting the Nrf2/HO-1 signaling pathway

S-ketamine alleviates carbon tetrachloride-induced hepatic injury and oxidative stress by targeting the Nrf2/HO-1 signaling pathway

Ketamine Improves Sepsis-induced Immunosuppression by Attenuating Intracellular Calcium Elevation in Rat Peripheral Mononuclear Cells

The effect of ketamine on Kupffer cell count in Wistar rat (Rattus norvegicus) model of sepsis

Contact Info

Product

Resources

About