The effect of Laminaria japonic polysaccharide on sperm characteristics and biochemical parameters in cryopreserved boar sperm

Hu, Jing-Hua; Sun, Xiaofang; Li, Qingwang; Zhang, Ting; Hu, Xiaochen; Hu, Jianhong; Wang, Liqiang

doi:10.1016/j.anireprosci.2013.03.015

Cited by 20 publications

(14 citation statements)

References 36 publications

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“…Despite the encouraging results found in this study, more experiments are still needed to further improve the quality of the post‐thawed boar sperm. There might be a synergistic effect on boar sperm cryopreservation between permeable antioxidants, which were used in this study (AP and FA), and impermeable antioxidants, which were used in other studies in our laboratory, such as Laminaria japonic polysaccharide (Hu et al., ) and Salvia miltiorrhiza polysaccharides (Shen, Jiang, Liu, & Li, ), since these polysaccharides can not only scavenge ROS but also unite with sperm membrane phospholipids, replacing the surrounding H 2 O (Crowe et al., ). Then the damage to the sperm caused by the formation of ice crystals will be reduced during the period of freezing.…”

Section: Discussionmentioning

confidence: 97%

Combined effect of apigenin and ferulic acid on frozen‐thawed boar sperm quality

Pei

et al. 2018

Animal Science Journal

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The main aim of the present study was to evaluate the cryoprotective effect of apigenin (AP) and ferulic acid (FA) on boar sperm during cryopreservation. AP and FA were both demonstrated to be high-efficiency antioxidants and had not previously been used to protect sperm from cryodamage. As boar sperm is sensitive to oxidative stress, suitable antioxidants are still needed for improving frozen-thawed sperm quality. With this purpose, semen samples coming from five boars were used in this study. Ejaculates of five boars were mixed and split into 16 aliquots, in which different doses of AP and FA were added separately or together. The motility, the plasma membrane integrity, the mitochondrial activity, the acrosomal integrity, the antioxidase activities and the malondialdehyde concentration of the frozen-thawed boar sperm were assessed. The results suggested that both AP and FA significantly improved the frozen-thawed boar sperm quality in all these aspects when they were added to the freezing extender separately, while the highest improvement was recorded when the extender was supplemented with 0.1 mmol/L AP plus 0.15 mmol/L FA. These findings demonstrated that supplementation of freezing extender with both AP and FA had a combined, beneficial effect on frozen-thawed boar sperm.

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Section: Discussionmentioning

confidence: 97%

Combined effect of apigenin and ferulic acid on frozen‐thawed boar sperm quality

Pei

et al. 2018

Animal Science Journal

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“…), Laminaria japonic polysaccharide (Hu et al. ), alginate (Hu et al. ) and fennel ( Foeniculum vulgare ; Malo et al.…”

Section: Cryopreservation Extendersmentioning

confidence: 99%

“…There are other additives that, despite not being antioxidants or CLC, also counteract the cryodamage, increase post-thaw sperm function and survival and improve the antioxidant defence system. These substances are docosahexaenoic acid (Kaeoket et al 2010b), Laminaria japonic polysaccharide (Hu et al 2013), alginate ) and fennel (Foeniculum vulgare; Malo et al 2012b).…”

Section: Other Additivesmentioning

confidence: 99%

Recent Advances in Boar Sperm Cryopreservation: State of the Art and Current Perspectives

Yeste

2015

Reprod Domestic Animals

112

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ContentsWhile sperm cryopreservation is the best technology to store boar semen for long-term periods, only 1% of all artificial inseminations (AI) conducted worldwide are made using frozen-thawed boar sperm. With the emergence of long-term extenders for liquid storage, the use of cryopreserved sperm in routine AI is less required. However, banks of boar semen contain cryopreserved sperm and planning inseminations in AI centres may benefit from the use of frozen-thawed semen. Therefore, there is an interest in the use of this technology to preserve boar sperm. In this regard, although the first attempts to cryopreserve boar semen date back to the seventies and this technology is still considered as optimal, some relevant improvements have been made in the last decade. After giving a general picture about boar sperm cryodamage, the present review seeks to shed light on these recent cryopreservation advances. These contributions regard to protein markers for predicting ejaculate freezability, sperm selection prior to start cryopreservation procedures, additives to freezing and thawing extenders, relevance of the AI-technique and insemination-toovulation interval. In conclusion, most of these progresses have allowed counteracting better boar sperm cryodamage and are thus considered as forward steps for this storage method. It is also worth noting that, despite being lower than fresh/ extended semen, reproductive performance outcomes following AI with frozen-thawed boar sperm are currently acceptable.

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“…Several studies have shown moderate to quite dramatic effects of a variety of antioxidants on in vitro sperm quality. Antioxidants such as BHT (Roca et al 2004), polysaccharides (Hu et al 2013), rice bran oil (Kaeoket et al 2012), rosemary extract (Malo et al 2010) and fennel extract (Malo et al 2012) were able to improve most measures of post-thaw sperm fertility. Others have added the target molecules of the ROS and certain PUFAs in the freezing extenders to improve post-thaw sperm quality (Kaeoket et al 2010b).…”

Section: Cryopreserved Sperm Additivesmentioning

confidence: 99%

The Fertility of Frozen Boar Sperm When used for Artificial Insemination

Knox

2015

Reprod Domestic Animals

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Contents One of the limits to practical use of frozen boar sperm involves the lowered fertility when used for artificial insemination. Years of studies have shown that 5–6 billion sperm (approximately 3 billion viable) used in single or multiple inseminations results in pregnancy rates most often between 60 and 70% and with litter sizes between nine and 10 pigs. Yet today, it is not uncommon for studies to report pregnancy rates from 70 to 85% and litter sizes with 11–12 pigs. While global statements about the incidence and reasons for higher fertility are not conclusive, incremental fertility improvements appear independently associated with use of a minimum number of viable sperm (1–2 billion), insemination timing that increases the probability that sperm will be present close to ovulation for groups of females, selection for boar sperm survival following cryopreservation, and modification of the freeze and thaw conditions using additives to protect sperm from oxidative damage. Studies show that techniques such as intrauterine and deep uterine insemination can provide an opportunity to reduce sperm numbers and that control of time of ovulation in groups of females can reduce the need for multiple inseminations and improve the chance for AI close to ovulation. However, optimal and consistent fertility with cryopreserved boar sperm may require a multifaceted approach that includes boar selection and screening, strategic use of additives during the freezing and thawing process, post‐thaw evaluation of sperm and adjustments in sperm numbers for AI, assessment of female fertility and ovulation induction for single insemination. These sequenced procedures should be developed and incorporated into a quality control system for improved fertility when using minimal numbers of cryopreserved boar sperm.

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The effect of Laminaria japonic polysaccharide on sperm characteristics and biochemical parameters in cryopreserved boar sperm

Cited by 20 publications

References 36 publications

Combined effect of apigenin and ferulic acid on frozen‐thawed boar sperm quality

Combined effect of apigenin and ferulic acid on frozen‐thawed boar sperm quality

Recent Advances in Boar Sperm Cryopreservation: State of the Art and Current Perspectives

The Fertility of Frozen Boar Sperm When used for Artificial Insemination

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