The effect of light on follicular development in laying hens

Abstract

Objective: The oxidative stress status and changes of chicken ovary tissue after shading were studied, which is to determine the mechanism of the effect of shading on follicular development.Methods: Twenty healthy laying hens (40 weeks old) with uniform body weight and the same laying rate were randomly divided into two groups (the shading group and normal light group).In the shading group, the cage was covered to reduce the light intensity inside the cage to 0 without affecting ventilation or food intake. The normal lighting group received no additional treatment. After 7 days of shading, oxidative stress related indicators and gene expression were detected.Results: Analysis of paraffin and ultrathin sections showed that apoptosis of ovarian granulosa cells increased significantly after light shading. ELISA (enzyme linked immunosorbent assay) results revealed that the levels of T-AOC (total antioxidant capacity), MDA (malondialdehyde), SOD (superoxide dismutase), GSH (glutathione), CAT (catalase) and other substances in the sera, livers, ovaries and follicular granulosa cells of laying hens increased significantly after shading for 7 days; and ROS (reactive oxygen species) levels in the livers of laying hens also increased significantly. ROS in the serum, ovarian and granulosa cell (GCs)also increased.After shading for 7 days, the levels of 8-OHdG (8-hydroxy-2 deoxyguanosine) in the sera and ovarian tissues of laying hens increased significantly. CCK-8 (cell counting kit-8) detection showed that the proliferation activity of GCs in layer follicles decreased after shading for 7 days; the expression level of the anti-apoptotic gene BCL2 (B-cell lymphoma-2) in ovarian tissue and follicular GCs was significantly reduced, and the expression levels of pro-apoptotic casp3 (caspase 3) and SOD, GPX2 (glutathione peroxidase 2) and CAT were all significantly increased. Conclusion:Oxidative stress induced by shading light has a serious inhibitory effect on follicular development during reproduction in laying hens.

Phosphoproteome Reveals Extracellular Regulated Protein Kinase Phosphorylation Mediated by Mitogen-Activated Protein Kinase Kinase-Regulating Granulosa Cell Apoptosis in Broody Geese

“…When we observed the ultrastructure of the ovaries, we found that a large number of granulosa cells were apoptotic during the brooding period [24], and the nuclei showed wrinkling and deformation, but the cell membranes were intact, and some of them showed foaming or apoptotic vesicles [25], while the apoptotic cells in the ovaries during the laying period were significantly fewer than those in the brooding period, and the antioxidant enzyme activities (SOD, CAT, GSH-PX) and oxidized free radicals (MDA, GSH, H 2 O 2 ) in the ovaries of geese during the brooding period were detected to be significantly higher. ROS can cause oxidative damage to cells, including the induction of apoptosis [26,27]. Therefore, we suggest that follicular atresia and apoptosis are closely related to oxidative stress in goose broods.…”

Melatonin alleviates ovarian function damage and oxidative stress induced by dexamethasone in the laying hens through FOXO1 signaling pathway

“…The separated plasma was stored at −20°C for subsequent studies. Levels of 8-OHdG, SOD, MDA, GSH-Px, CAT, MT as well as ROS were evaluated by ELISA with the help of a commercial kit (Shanghai Jianglai Biotechnology Co., Ltd., Shanghai, China), by following the protocol provided by the manufacturer ( Sihong et al, 2019 ; Hao et al, 2020b ; Cheng et al, 2021 ).…”

Female Germ Cell Development in Chickens and Humans: The Chicken Oocyte Enriched Genes Convergent and Divergent with the Human Oocyte

“…This phenomenon in older chickens is connected with a reduced rate of follicle recruitment for rapid growth and a lengthy period of follicular growth and development [ 49 ]. Several factors, including the environmental conditions and nutrition supply, influence the decrease in egg production in laying chickens [ 50 ]. However, oocyte apoptosis, a failure to maintain the ovarian reserve due to the excessive activation of primordial follicles for rapid growth, elevated levels of reactive oxygen species (an oxidative stress condition), the formation of a high number of atretic follicles, and a decline in liver function are factors primarily associated with the gradual decrease in, and even cessation of, egg production in older chickens [ 51 , 52 , 53 ].…”