The Effect of Light upon Development in Potato Tissue Slices

Hanebuth, William F.; Chasson, Robert M.

doi:10.1104/pp.49.5.857

Cited by 4 publications

(2 citation statements)

References 8 publications

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“…The inhibition of cytochrome oxidase by cyanide (6) should cause a cessation or reduction of ATP production, reducing over-all ATP to a level where the water permeability of the cell could be changed. However, reducing the level of ATP could also lower the concentration of CTP (2) to an inhibitory level.…”

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Reduction of Water Permeability in Potato Tuber Slices by Cyanide, Ammonia, 2,4-Dinitrophenol, and Oligomycin and Its Reverse by Adenosine 5′-Triphosphate and Cytidine 5′-Triphosphate

Stuart¹

1973

Plant Physiol.

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MATERIALS AND METHODS Five millimolar KCN reduced water permeability in 1-millimeter thick slices of potato tuber (Solanum tuberosum L.). One-tenth millimolar ATP and CTP prevented or reversed the reduced permeability. UTP and GTP were not effective. Five millimolar ammonium carbonate or 0.1 millimolar 2,4-dinitrophenol also reduced water permeability, but ATP and CTP were only partially effective in reversing the reduced permeability. Oligomycin, 5 micrograms per milliliter, reduced water permeability, and the reduction was reversed by ATP and CTP. ATP and CTP appear to be involved in maintaining the structure of water pathways into the cell.Respiration inhibitors and uncouplers such as ammonia (25), azide (15, 20, 27), cyanide (3,16,18,27,28), carbonyl cyanide m-chlorophenylhydrazone (1, 3), 2,4-dinitrophenol (18, 32), fluoride (27, 32), iodoacetate (27, 32), arsenate (27), CO,(5,12) Uniform disks, 14 mm in diameter and 1 mm thick, were cut from potato tubers (Solanum tuberosum L. var. Russet). The disks were washed in tap water, usually 10 to 15 min, until the starch and cell debris were removed from the cut surface, rinsed twice in distilled water, and stored in aerated distilled water at 5 C until needed an hour or two later.Six disks (about 1 g fresh weight) were placed in 50 ml of the sodium salt of 0.1 mm ATP, CTP, GTP, or UTP and gently shaken for 45 min. The appropriate amount of ammonia, cyanide, DNP,2 or oligomycin was then added, and the shaking was continued for an additional 45 min or for only 10 min in the ammonia solution. At the end of this time, the disks were removed from the solution, blotted between Whatman No. 1 filter paper (50 g pressure for 5 sec), and weighed to the nearest milligram. The disks were then placed in a 13 atm (90 g/liter) solution (measured with the thermocouple psychrometer) of mannitol containing the appropriate amount of nucleotide, inhibitor, etc.; shaken for 10 min; removed from the mannitol; blotted as before; and reweighed. The difference between the first and second weighing was taken as the amount of water lost.Oligomycin (Sigma Chemical Company; 15% oligomycin A, 85% oligomycin B)' was dissolved in absolute ethanol to make a concentration of 10 mg/ml. Aliquots of this stock solution were used to make a final concentration of 5 jug/ml. For the oligomycin experiments the same concentration of absolute ethanol was added to the controls and mannitol solution as was added in the test solutions.In half of the experiments the inhibitor or uncoupler was added first and the ATP, CTP, etc. was added later. When needed, potassium phosphate buffer (0.01 M) was used to control pH. The control experiments were conducted without adding the inhibitors. All experiments were conducted at 23 C.Each experiment was repeated five times, and the results were analyzed statistically for significance using Duncan's new multiple range test (4). Statistical comparisons between experiments were not made.'Abbreviation: DNP: 2,4-dinitrophenol.

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Reduction of Water Permeability in Potato Tuber Slices by Cyanide, Ammonia, 2,4-Dinitrophenol, and Oligomycin and Its Reverse by Adenosine 5′-Triphosphate and Cytidine 5′-Triphosphate

Stuart¹

1973

Plant Physiol.

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“…As the tissue ages, the relative contributions of the tricarboxylic acid cycle and the pentose phosphate metabolic pathways are changing, although the details of these events are not completely clear (Ap Rees and Beevers I960, Romberger and Norton 1961, Laties 1964, Ricardo and Ap Rees 1972. In addition, aged tissue may develop modifications of the terminal portions of the electron transport chain as shown by a rapid loss of cyanide sensitivity during the aging process (Hackett et al 1960, Click and Hackett 1963, Laties 1967, Hanebuth and Chasson 1972. This paper reports on the time course of sulfate uptake in physiologically aged potato tuber slices.…”

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confidence: 99%

Sulfate Uptake and Respiration of Aging Potato Discs Modified by Malonic Acid and Ultraviolet Radiation

Hanebuth

Chasson

Pittman

1974

Physiologia Plantarum

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The tinne course of sulfate uptake in relation to respiration is shown for aging slices of potato, Solanum tuberosum L. cv. Russet. For comparative purposes, a time course for phosphate uptake is also shown. Malonic acid depressed both the respiratory rise and the sulfate accumulating ability of aging discs. A low dose of ultraviolet radiation given at the onset of aging reduced sulfate uptake by one-half without lowering respiration. We hypothesize that sulfate uptake is controlled by at least two mechanisms: one associated with oxidative metabolism and the other mediated by protein synthesis.

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Effect of the Origin of Explant on Callus Initiation and Differentiation in Potato

Quraishi

John

Rossignol-Bancilhon

et al. 1987

Biotechnology in Agriculture and Forestry

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The Effect of Light upon Development in Potato Tissue Slices

Cited by 4 publications

References 8 publications

Reduction of Water Permeability in Potato Tuber Slices by Cyanide, Ammonia, 2,4-Dinitrophenol, and Oligomycin and Its Reverse by Adenosine 5′-Triphosphate and Cytidine 5′-Triphosphate

Reduction of Water Permeability in Potato Tuber Slices by Cyanide, Ammonia, 2,4-Dinitrophenol, and Oligomycin and Its Reverse by Adenosine 5′-Triphosphate and Cytidine 5′-Triphosphate

Sulfate Uptake and Respiration of Aging Potato Discs Modified by Malonic Acid and Ultraviolet Radiation

Effect of the Origin of Explant on Callus Initiation and Differentiation in Potato

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