The effect of low concentrations of an organic lead compound on the transport and release of putative neurotransmitters

Bondy, Stephen C.; Harrington, Marilyn E.; Anderson, C. L.; Prasad, Kedar N.

doi:10.1016/0378-4274(79)90104-8

Cited by 19 publications

(7 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This specificity has been substantiated by a series of studies analogous to those reported here but measuring six neurotransmitter or neuromodulator sites within the brain (Agrawal et al, 1980;Bondy et al, 1980) It is known that the dopamine system is especially sensitive to a variety of neurotoxic agents including manganese (Goldman, 1972) and lead compounds (Bondy et al, 1979). It is important to delineate further the selectivity of the effects reported here by assay of receptors for other neurotransmitters in acrylamide-treated animals.…”

Section: Ko (Nm)supporting

confidence: 53%

The effects of acrylamide treatment upon the dopamine receptor

Agrawal

Squibb

Bondy

1981

Toxicology and Applied Pharmacology

View full text Add to dashboard Cite

. (1981). Toxicol. Appl. Pharmacol. 58,[89][90][91][92][93][94][95][96][97][98][99]. The binding of tritiated spiroperidol to striatal membranes prepared from acrylamide-treated male 6-week-old rats and matched controls has been studied. This binding has highaffinity characteristics and is stereospecific and reversible. Equilibrium was attained within 15 min at 37°C. The extent of binding was much more pronounced in the striatum than in any other brain region and was not detectable within the cerebellum or spinal cord. Regional distribution of binding and competition studies with other pharmacologic agents suggested a correspondence between the location of ligand-membrane complex formation and the dopamine receptor. Twenty-four hours after a single oral administration of acrylamide there was a significant increase in [ 3 H]spiroperidol binding at all acrylamide doses tested (50, 100, and 200 mJYkg body weight). However, there was no significant change in striatal dopamine levels of treated animals. Kinetic analysis of animals treated with 100 mg acrylamide/kg suggested increased affinity of receptors of treated animals toward the labeled ligand. Receptor densicy was only slightly elevated in experimental animals. Effects were also studied in rats that had received 10, 20, or 30 mg/kg acrylamide daily for 10 days. Twentyfour hours after the last dose there was a major increase in spiroperidol binding in treated animals. Scatchard plot analysis again revealed that this change was largely attributable to a change in the dissociation constant of the binding interaction but also there was an increase in the overall number of receptor sites. Nonna! values were restored within 8 days after cessation of dosing. This illustrates that, under the conditions of this experiment, the effect of acrylamide on a central neurotransmitter system is reversible.

show abstract

Section: Ko (Nm)supporting

confidence: 53%

The effects of acrylamide treatment upon the dopamine receptor

Agrawal

Squibb

Bondy

1981

Toxicology and Applied Pharmacology

View full text Add to dashboard Cite

show abstract

“…While temperature did not affect [ 3 H]GABA release in our system, NELSON-KRAUSE & How ARD (1978) found that K + or calcium-ionophore related release of [3H]GABA from rat brain synaptosomes was reduced but not abolished by metabolic inhibitors. We have found that dibutyryl lead acetate, a neurotoxic compound, may actually enhance release of accumulated compounds when it is present at low concentrations (below 10-6 M. BONDY, HARRINGTON, ANDERSON & PRASAD, 1978).…”

Section: Lrifluence Of Temperature and Of Developmental Agementioning

confidence: 85%

Calcium-dependent release of putative neurotransmitters in the chick visual system

Bondy¹,

Harrington

1979

Neuroscience

Self Cite

View full text Add to dashboard Cite

“…The cellular and molecular mechanisms of CH 3 HgCl-induced damage to nervous tissue are unknown. 10 µM CH 3 HgCl inhibits the high affinity uptake of certain putative neurotransmitters (dopamine, glycine, choline, glutamate and GABA) in adult mouse brain homogenates [4]. In rats, long-term administration of methylmercury decreases the levels of cortical acetylcholine and brainstem 5-hydroxytryptamine and norepinephrine [5].…”

Section: Introductionmentioning

confidence: 99%

“…The concentration of CH 3 HgCl required to inhibit choline uptake in NB cells was 1/10 of that required for its inhibition in adult mouse brain homogenates [4]. The reduced uptake of choline may in part account for a decreased level of acetylcholine in the cortical area of rat brain [5].…”

mentioning

confidence: 99%

“…The reduced uptake of choline may in part account for a decreased level of acetylcholine in the cortical area of rat brain [5]. The energy-dependent uptake of dopamine and GABA was not shown in NB cells, but was demonstrable in adult mouse brain homogenates [4], due in part perhaps to the fact that this clone of NB is of cholinergic type with relatively undifferentiated cells.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Effects of methylmercuric chloride on high affinity uptake of certain putative neurotransmitters in neuroblastoma and glioma cell cultures

1979

View full text Add to dashboard Cite

SUMMARYThe effect of methylmercuric chloride (CH 3 HgCl) on high affinity uptake of certain putative neurotransmitters on mouse neuroblastoma (NBE-) and rat glioma (C-6) cells in culture 3 days after treatment was studied. The energydependent accumulation of radioactive choline was decreased in NB cells after treatment with CH 3 HgCl (1 µM), whereas the accumulation of radioactive glycine or glutamate was increased. The uptake of radioactive glycine or glutamate in glioma cells was decreased after treatment with CH 3 HgCl (0.3 µM). High affinity uptake of choline was not demonstrable in glioma cells. Low concentrations of CH 3 HgCl affect the accumulation of glycine and glutamate in glioma and NB cells in an opposite manner.

show abstract

The effect of low concentrations of an organic lead compound on the transport and release of putative neurotransmitters

Cited by 19 publications

References 20 publications

The effects of acrylamide treatment upon the dopamine receptor

The effects of acrylamide treatment upon the dopamine receptor

Calcium-dependent release of putative neurotransmitters in the chick visual system

Effects of methylmercuric chloride on high affinity uptake of certain putative neurotransmitters in neuroblastoma and glioma cell cultures

Contact Info

Product

Resources

About