The effect of low-level laser irradiation on hyperglycemia-induced inflammation in human gingival fibroblasts

Lee, Kun-Tsung Denzel; Chiang, Min‐Hsuan; Chen, Ping-Ho; Ho, Mei-Ling; Lee, Hong‐Zin; Lee, Huey-Er; Wang, Yan‐Hsiung

doi:10.1007/s10103-018-2675-6

Cited by 26 publications

(9 citation statements)

References 46 publications

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“…These articles were evaluated based on the eligibility criteria of the review. Additional studies were excluded due to the following reasons: an inappropriate study design or article type in 9 studies (15,16,21–27), LLLT utilized, as an adjunct to a surgical intervention or in the supportive periodontal therapy phase in 3 studies (28–30), in vitro or in vivo animal studies utilized in 3 papers (31–33), participants with systemic diseases recruited in 3 studies (34–39), no utilization of PBM in 11 studies (40–50), and LLLT utilized either; as a mono‐therapeutic agent or in combination with aPDT in 3 studies (51–53) (inter‐reviewer agreement, κ = 1). Hence, out of 52 full text articles, 35 articles were excluded, due to the abovementioned reasons, while the remaining 17 articles were included and analyzed in the present systematic review.…”

Section: Resultsmentioning

confidence: 99%

Effectiveness of Photobiomodulation as an Adjunct to Nonsurgical Periodontal Therapy in the Management of Periodontitis‐ A Systematic Review of in vivo Human Studies

Dalvi

Benedicenti

Hanna

2020

Photochem & Photobiology

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Photobiomodulation therapy (PBMT) has anti‐inflammatory, analgesic and regenerative properties. This systematic review aimed to critically appraise the published data of in vivo human randomized clinical trials (RCTs), and present a comprehensive overview of the efficacy of PBMT, as an adjunct to the nonsurgical periodontal therapy (NSPT) in the management of periodontitis. The systematic review protocol is registered in the Prospective Register Of Systematic Reviews (PROSPERO) (http://www.crd.york.ac.uk/PROSPERO/; ref CRD 42020169108). With the help of the appropriate key words, structured electronic and manual search strategies were applied to gather the relevant published data on in vivo human RCTs based on this topic. Seventeen papers that met the eligibility criteria were included in this review and subjected to a qualitative assessment. Current evidence lacks adequate information regarding the photobiomodulation (PBM) dosimetry, which is fundamental in establishing a standardized and replicable protocol for future researches. Furthermore, substantial discrepancies in the study methodology and a high risk of bias, arising from the majority of the included papers, abet to the inferior quality of these studies. Ultimately, there is an urgent necessity to conduct further well‐designed RCTs in order to determine the effectiveness of PBMT, if any, by taking into consideration the abovementioned confounding factors.

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Section: Resultsmentioning

confidence: 99%

Effectiveness of Photobiomodulation as an Adjunct to Nonsurgical Periodontal Therapy in the Management of Periodontitis‐ A Systematic Review of in vivo Human Studies

Dalvi

Benedicenti

Hanna

2020

Photochem & Photobiology

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“…During the last few decades, numerous studies demonstrated the beneficial effects of light in the form of PBM for the treatment of various pathologiesalthough the exact cellular mechanisms responsible for the positive effects are not fully understood yet. Beside the promotion of wound healing and angiogenesis 27 and the reduction of inflammatory reactions, 28 the enhancement of bone formation represents an important field of application for PBMT. PBMT of bone tissue seems to improve the results of fracture repair, periodontal tissue, implant osseointegration and bone reconstruction with or without biomaterials (reviewed in ref.…”

Section: Introductionmentioning

confidence: 99%

Cross-modality imaging of bisphosphonate-treated murine jawbones

Reier

Turyanskaya

Heimel

et al. 2021

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“…GFs are commonly used to explore the effects of PBM as a model for periodontitis. In particular, near infra-red (NIR) light has been observed to promote cell proliferation [15][16][17] and decrease markers for inflammation [18][19][20] from GFs. Comparatively, the effects of blue light have only been reported by relatively few authors, where high doses of blue light (> 100 J/cm 2 ) induce increased oxidative stress [21,22].…”

Section: Introductionmentioning

confidence: 99%

Photobiomodulation of oral fibroblasts stimulated with periodontal pathogens

et al. 2021

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Photobiomodulation (PBM) utilises light energy to treat oral disease, periodontitis. However, there remains inconsistency in the reporting of treatment parameters and a lack of knowledge as to how PBM elicits its molecular effects in vitro. Therefore, this study aimed to establish the potential immunomodulatory effects of blue and near infra-red light irradiation on gingival fibroblasts (GFs), a key cell involved in the pathogenesis of periodontitis. GFs were seeded in 96-well plates in media + / − Escherichia coli lipopolysaccharide (LPS 1 μg/ml), or heat-killed Fusobacterium nucleatum (F. nucleatum, 100:1MOI) or Porphyromonas gingivalis (P. gingivalis, 500:1MOI). Cultures were incubated overnight and subsequently irradiated using a bespoke radiometrically calibrated LED array (400–830 nm, irradiance: 24 mW/cm2 dose: 5.76 J/cm2). Effects of PBM on mitochondrial activity (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and adenosine triphosphate (ATP) assays, total reactive oxygen species production (ROS assay) and pro-inflammatory/cytokine response (interleukin-8 (IL-8) and tumour growth factor-β1 (TGFβ1)) were assessed 24 h post-irradiation. Data were analysed using one-way ANOVA followed by the Tukey test. Irradiation of untreated (no inflammatory stimulus) cultures at 400 nm induced 15%, 27% and 13% increases in MTT, ROS and IL-8 levels, respectively (p < 0.05). Exposure with 450 nm light following application of P. gingivalis, F. nucleatum or LPS induced significant decreases in TGFβ1 secretion relative to their bacterially stimulated controls (p < 0.001). Following stimulation with P. gingivalis, 400 nm irradiation induced 14% increases in MTT, respectively, relative to bacteria-stimulated controls (p < 0.05). These findings could identify important irradiation parameters to enable management of the hyper-inflammatory response characteristic of periodontitis.

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The effect of low-level laser irradiation on hyperglycemia-induced inflammation in human gingival fibroblasts

Cited by 26 publications

References 46 publications

Effectiveness of Photobiomodulation as an Adjunct to Nonsurgical Periodontal Therapy in the Management of Periodontitis‐ A Systematic Review of in vivo Human Studies

Effectiveness of Photobiomodulation as an Adjunct to Nonsurgical Periodontal Therapy in the Management of Periodontitis‐ A Systematic Review of in vivo Human Studies

Cross-modality imaging of bisphosphonate-treated murine jawbones

Photobiomodulation of oral fibroblasts stimulated with periodontal pathogens

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