The effect of lysolecithin and related compounds on the swelling of isolated mitochondria

Witter, Robert F.; Cottone, Mary A.

doi:10.1016/0006-3002(56)90165-2

Cited by 28 publications

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“…Aging or freezing of the mitochondria or the presence of calcium also activates Mg-ATPase and inhibits DNP-ATPase (3,14,17), and these conditions promote as well the swelling of these organelles (18)(19)(20). It would thus be of interest to determine whether a similar correlation could be made in the case of the surface active agents.…”

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“…Therefore, a study was made of the effects of different types of detergents on the activities of Mg-ATPase and DNP-ATPase and on the swelling of the isolated mitochondria. The latter was estimated by determining the decrease in the turbidity of mitochondria suspended in solutions of sucrose containing various detergents (18,21). The rate of decrease in turbidity was assumed to be a measure of the rate of swelling of the mitochondria (18,21).…”

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“…The latter was estimated by determining the decrease in the turbidity of mitochondria suspended in solutions of sucrose containing various detergents (18,21). The rate of decrease in turbidity was assumed to be a measure of the rate of swelling of the mitochondria (18,21). In addition, these results were checked by observation of the mitochondria under the phase microscope.…”

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“…The compounds tested induded anionic and cationic detergents with either aromatic or alkyl side chains and various non-ionic detergents of the polyoxyethylene type, including those containing sorbitan mono-oleate or monolaurate groups (tween 80 or 20), amine groups (anatarox G100), and nonyl phenoxy groups (igepal). The swelling of isolated mitochondria is known to be influenced by the pH of the medium, the concentration of sucrose, and the presence of calcium ions (18)(19)(20). Therefore in the present experiment the effect of the various detergents was determined at pH values ranging from 6.2 to 8.0 and in the presence or absence of the complexing agent versene (18).…”

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“…The swelling of isolated mitochondria is known to be influenced by the pH of the medium, the concentration of sucrose, and the presence of calcium ions (18)(19)(20). Therefore in the present experiment the effect of the various detergents was determined at pH values ranging from 6.2 to 8.0 and in the presence or absence of the complexing agent versene (18). Also the tests were carried out in 0.44 sucrose, since at this concentration possible effects of the osmotic pressure of the solution are minimized (iS).…”

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Effect of Synthetic Detergents on the Swelling and the ATPase of Mitochondria Isolated from Rat Liver

Witter

Mink

1958

The Journal of Cell Biology

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A study was made of the effects of various types of detergents on the swelling of isolated mitochondria and on mitochondrial ATPases which are activated by Mg or DNP respectively. The rate of swelling was measured in the Beckman spectrophotometer by following the decrease in turbidity of dilute suspensions of these organelles. It was found that non-ionic detergents containing a nonyl phenoxy side chain or anionic detergents caused swelling of the mitochondria and activation of Mg-ATPase. On the other hand, cationic detergents promoted the clumping of mitochondria and did not activate Mg-ATPase. DNP-ATPase was inhibited by all of the detergents tested. It would appear from these observations that the inhibition of DN'P-ATPase is not related to a gross change in the morphology of the organelles; in contrast, the activation of Mg-ATPase definitely is correlated with swelling of the isolated mitochondria. These data also suggest that the ionic detergents combine with charged sites on the protein moiety of the lipoprotein in the mitochondrial surface, whereas the non-ionic detergents form inclusion compounds with the lipide moiety, thereby altering the mitochondrial structure and permeability.

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