The effect of macrophages on an atmospheric pressure plasma-treated titanium membrane with bone marrow stem cells in a model of guided bone regeneration

Toyama, Naoto; Tsuchiya, Shunji; Kamio, Hisanobu; Okabe, Kazuto; Kuroda, Kensuke; Peng, Cong; Hibi, Hideharu

doi:10.1007/s10856-020-06412-7

Cited by 3 publications

(5 citation statements)

References 38 publications

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“…Biology 2020, 9, x FOR PEER REVIEW 3 of 15 characteristic exhibited only by a fraction of BMSC [19,20]. Several studies have clearly demonstrated that BMSCs have great potency for promoting the regeneration of bone defects in animal models due to their high capacity for self-renewal and multipotentiality for differentiation [3][4][5]21,22]. The aim of this work was to investigate the effect of CGF in promoting the osteogenic differentiation of hBMSC in vitro.…”

Section: Cgf Increased Alp Activity In Hbmscmentioning

confidence: 99%

“…The "stemness" characteristic phenotype of BMSC is based on their ability to form heterotopic bone and bone marrow organ during serial transplantation in vivo, a characteristic exhibited only by a fraction of BMSC [19,20]. Several studies have clearly demonstrated that BMSCs have great potency for promoting the regeneration of bone defects in animal models due to their high capacity for self-renewal and multipotentiality for differentiation [3][4][5]21,22].…”

Section: Introductionmentioning

confidence: 99%

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Concentrated Growth Factors (CGF) Induce Osteogenic Differentiation in Human Bone Marrow Stem Cells

Rochira

Siculella

Damiano

et al. 2020

Biology

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Bone regeneration is a complex process regulated by several factors that control overlapping biological processes, coordinating interactions among distinct cell populations. There is a great interest in identifying new strategies for inducing osteogenesis in a safe and efficient manner. Concentrated Growth Factor (CGF) is an autologous blood derived product obtained by centrifugation of venous blood following the procedure set on the Silfradent device. In this study the effects of CGF on osteogenic differentiation of human Bone Marrow Stem Cells (hBMSC) in vitro have been investigated; hBMSC were cultured with CGF or osteogenic medium, for 21 days. The osteogenic differentiation was evaluated measuring alkaline phosphatase (ALP) enzyme activity, matrix mineralization by alizarin red staining and through mRNA and protein quantification of osteogenic differentiation markers by Real-time PCR and Western blotting, respectively. The treatment with CGF stimulated ALP activity and promoted matrix mineralization compared to control and seems to be more effective than osteogenic medium. Also, hBMSC lost mesenchymal markers and showed other osteogenic features. Our study showed for the first time that CGF alone is able to induce osteogenic differentiation in hBMSC. The application of CGF on hBMSC osteoinduction might offer new clinical and biotechnological strategies in the tissue regeneration field.

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Section: Cgf Increased Alp Activity In Hbmscmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Concentrated Growth Factors (CGF) Induce Osteogenic Differentiation in Human Bone Marrow Stem Cells

Rochira

Siculella

Damiano

et al. 2020

Biology

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“…In 2020, Toyama et al reported an atmospheric pressure plasma (APP)-treated Ti membrane and analyzed its effect on the differentiation of BMSCs [51]. In this study, the APP-coated Ti membrane was identified to increase cell migration and gene-level expression of osteogenic markers; however, the suppression of mineralization was observed in an in vitro experiment.…”

Section: No Significant Differencementioning

confidence: 77%

“…In this study, the APP-coated Ti membrane was identified to increase cell migration and gene-level expression of osteogenic markers; however, the suppression of mineralization was observed in an in vitro experiment. Furthermore, in the in vivo experiment, the new bone formation was not significantly different between APP-coated and noncoated Ti membranes [51].…”

Section: No Significant Differencementioning

confidence: 77%

Various Coated Barrier Membranes for Better Guided Bone Regeneration: A Review

Kim

Park

2022

Coatings

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A good barrier membrane is one of the important factors for effective guided bone/tissue regeneration (GBR/GTR) in the case of periodontal bone defects. Several methods are being discussed to overcome and improve the shortcomings of commercially available membranes. One of the methods is to coat the membrane with bioactive materials. In this study, 41 studies related to coated membranes for GBR/GTR published in the last 5 years were reviewed. These studies reported coating the membrane with various bioactive materials through different techniques to improve osteogenesis, antimicrobial properties, and physical/mechanical properties. The reported studies have been classified and discussed based on the purpose of coating. The goal of the most actively studied research on coating or surface modification of membranes is to improve new bone formation. For this purpose, calcium phosphate, bioactive glass, polydopamine, osteoinduced drugs, chitosan, platelet-rich fibrin, enamel matrix derivatives, amelotin, hyaluronic acid, tantalum, and copper were used as membrane coating materials. The paradigm of barrier membranes is changing from only inert (or biocompatible) physical barriers to bioactive osteo-immunomodulatory for effective guided bone and tissue regeneration. However, there is a limitation that there exists only a few clinical studies on humans to date. Efforts are needed to implement the use of coated membranes from the laboratory bench to the dental chair unit. Further clinical studies are needed in the patients’ group for long-term follow-up to confirm the effect of various coating materials.

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“…Surface modification of GBR materials can also induce M2 polarization of macrophages and affect osteogenesis. Toyama et al reported the application of an atmospheric-pressure plasma-treated titanium (APP-Ti) membrane in rat calvaria defect repair [95] . Compared to the untreated Ti membrane (N-Ti), more M1 and M2 polarization occurred in macrophages cultured on the APP-Ti membrane.…”

Section: Immunoregulatory Materials To Induce Osteogenesismentioning

confidence: 99%

Recent advances in biofunctional guided bone regeneration materials for repairing defective alveolar and maxillofacial bone: A review

Wang

Feng²,

Liu³

et al. 2022

Japanese Dental Science Review

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The effect of macrophages on an atmospheric pressure plasma-treated titanium membrane with bone marrow stem cells in a model of guided bone regeneration

Cited by 3 publications

References 38 publications

Concentrated Growth Factors (CGF) Induce Osteogenic Differentiation in Human Bone Marrow Stem Cells

Concentrated Growth Factors (CGF) Induce Osteogenic Differentiation in Human Bone Marrow Stem Cells

Various Coated Barrier Membranes for Better Guided Bone Regeneration: A Review

Recent advances in biofunctional guided bone regeneration materials for repairing defective alveolar and maxillofacial bone: A review

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