The effect of magnesium and vitamin E pre-treatments on irradiation-induced oxidative injury of cardiac and pulmonary tissues in rats: a randomized experimental study

Abstract

The effect of magnesium and vitamin E pre-treatments on irradiationinduced oxidative injury of cardiac and pulmonary tissues in rats: a randomized experimental studyProfilaktik magnezyum ve E vitamini uygulamasının sıçanlarda ışınlamaya bağlı kalp ve akciğer dokusunda gelişen oksidan hasara etkileri: Randomize deneysel bir çalışma ABSTRACTObjective: The aim of this study was to investigate the effect of pre-treatment with the free radical scavenging molecules, magnesium and vitamin E, on lipid peroxidation to limit radiation-induced heart and lung injury. Methods: Female Sprague-Dawley rats were divided into 4 groups by a simple randomization method as saline-treated control (n=4), salinetreated irradiated (IR; n=6), magnesium sulphate-treated irradiation (IR) (Mg+IR; n=6) and vitamin E-treated IR (vit E+IR; n=6), respectively. The animals were given either saline, Mg (600mg/kg/day) or vit E (100 mg/kg/day) intraperitoneally for five days prior to irradiation. Twelve hours after the fifth injection, animals in irradiation groups were irradiated to 20 Gy using 6 MV photons in linear accelerator. Twenty-four hours later cardiac and lung tissue samples were obtained for determination of myeloperoxidase activity (MPO), malondialdehyde (MDA) levels, and luminol and lucigenin levels measured by chemiluminescence (CL) methods. Results: No significant changes were observed between cardiac and pulmonary MDA and CL results of the experimental groups. However, cardiac and pulmonary MPO activities in the saline-treated IR group were increased as compared to control group (p<0.05 for all), while in the Mg-pretreated and vit E pretreated groups neutrophil infiltration was reduced, reaching to statistical significance only in the Mg-pretreated group (p<0.05). Conclusion: Prophylactic use of magnesium sulfate has limited the infiltration of neutrophils to both the cardiac and pulmonary tissues at the early 24 h of irradiation. However, how limiting neutrophils as the sources of free radicals and inflammatory mediators would alter oxidative stress of heart and lung tissues in the long-term is not clear yet. (Anadolu Kardiyol Derg 2012; 12: 508-14) Key words: Heart, irradiation, lung, magnesium, radioprotection, vitamin E Original Investigation Özgün Araşt›rma 508ÖZET Amaç: Serbest radikal süpürücü moleküller olan magnezyum sülfat ve E vitamininin profilaktik kullanımının radyasyonun tetiklediği kalp ve akciğer hasarının önlenmesinde lipit peroksidasyonu üzerindeki etkileri incelendi. Yöntemler: Sprague-Dawley türü dişi sıçanlar basit rastgele randomizasyon yöntemi kullanılarak fizyolojik tuzlu su uygulanan kontrol (n=4), tek başına radyasyon (IR; n=6), radyasyon+magnezyum (Mg+IR; n=6) ve radyasyon+vitamin E (vit E+IR; n=6) ön-tedavisine göre gruplandırıldı. Fizyolojik tuzlu su, Mg (600 mg/kg/gün) ya da vit E (100 mg/kg/gün) radyasyondan önce 5 gün boyunca intraperitonal olarak uygulandı. Son enjeksiyondan 20 saat sonra radyasyon uygulanacak tüm gruplardaki sıçanlara 6 MV foton enerjisi ile 20 Gy iyonizan radyasyon uygulandı. ...

Oxidative Stress in Radiation-Induced Cardiotoxicity

“…Therefore, Vit E can reduce the risk of coronary heart disease [14]. Many studies have identified the antioxidant and antiatherosclerotic properties of vitamin E in animals [71][72][73][74]. In 2001, Ferre et al found that a diet containing vitamin E, palm oil, or sunflower oil would reduce the range of pathological changes in the aorta [71].…”

Ophiopogon japonicus inhibits radiation-induced pulmonary inflammation in mice

“…Hydroxyproline is an important component of collagen which correlates to the severity of the lung fibrosis (17), and MDA is an important lipid peroxide that reflects the damage caused by oxygen free radical (18). Our results showed that the content of hydroxyproline and MDA in lung tissue in the radiation group was significantly higher than that in the control group.…”