The effect of maltose on dextran yield and molecular weight distribution

Rodrigues, Sueli; Lona, Liliane Maria Ferrareso; Franco, Telma Teixeira

doi:10.1007/s00449-005-0002-7

Cited by 30 publications

(42 citation statements)

References 18 publications

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“…Statistical significance was evaluated by ANOVA and F test (Table 3) considering a confidence interval of 95%. All fitted models were statistically significant since F calculated [ F listed [16]. The regression coefficients were also good for all fitted models (R 2 [ 0.90).…”

Section: Discussionmentioning

confidence: 77%

Effect of pH and Temperature on Enzyme Activity of Chitosanase Produced Under Solid Stated Fermentation by Trichoderma spp.

et al. 2011

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Trichoderma strains were extensively studied as biocontrol agents due to their ability of producing hydrolytic enzymes, which are considered key enzymes because they attack the insect exoskeleton allowing the fungi infection. The present work aimed to evaluate the ability of chitosanase production by four Trichoderma strains (T. harzianum, T. koningii, T. viride and T. polysporum) under solid stated fermentation and to evaluate the effect of pH and temperature on enzyme activity. pH strongly affected the enzyme activity from all tested strains. Chitosanase from T. harzianum and T. viride presented optimum activity at pH 5.0 and chitosanase from T. koningii and T. polysporum presented optimum activity at pH 5.5. Temperature in the range of 40-50°C did not affect enzyme activity. T. polysporum was found as the most promising strain to produce chitosanase with maximal enzyme activity of about 1.4 IU/gds, followed by T. viride (*1.2 IU/gds) and T. harzianum (1.06 IU/gds).

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Section: Discussionmentioning

confidence: 77%

Effect of pH and Temperature on Enzyme Activity of Chitosanase Produced Under Solid Stated Fermentation by Trichoderma spp.

et al. 2011

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“…The crude fermented broth free of cells (100 lL) was mixed with 400 lL of a 10% (w/v) sucrose solution in an acetate buffer pH 5.2 (20 mM containing 0.05 g/L of CaCl 2 ) and then incubated for 1 h at 30°C. The reaction was stopped adding three volumes of ethanol 96% (v/v) [18]. The enzyme activity was expressed in DSU/10 lL (dextransucrase unit/lL).…”

Section: Enzyme Activity Assaymentioning

confidence: 99%

Dextransucrase production using cashew apple juice as substrate: effect of phosphate and yeast extract addition

Chagas

Honorato

Pinto

et al. 2007

Bioprocess Biosyst Eng

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Cashew apples are considered agriculture excess in the Brazilian Northeast because cashew trees are cultivated primarily with the aim of cashew nut production. In this work, the use of cashew apple juice as a substrate for Leuconostoc mesenteroides cultivation was investigated. The effect of yeast extract and phosphate addition was evaluated using factorial planning tools. Both phosphate and yeast extract addition were significant factors for biomass growth, but had no significant effect on maximum enzyme activity. The enzyme activities found in cashew apple juice assays were at least 3.5 times higher than the activity found in the synthetic medium. Assays with pH control (pH = 6.5) were also carried out. The pH-controlled fermentation enhanced biomass growth, but decreased the enzyme activity. Crude enzyme free of cells produced using cashew apple juice was stable for 16 h at 30 degrees C at a pH of 5.0.

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“…Rodrigues [21] employed maltose as acceptor to study dextran synthesis in the submerged culture of Leuconostoc mesenteroides B512F. The author found that maltose was unable to control and reduce dextran molecular weight distribution and suggested that acceptor reaction could occur at a unique and separated site.…”

Section: Resultsmentioning

confidence: 98%

Effects of phytohormones on mycelial growth and exopolysaccharide biosynthesis of medicinal mushroom Pellinus linteus

Guo

Zou

Sun

2009

Bioprocess Biosyst Eng

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Effects of phytohormones including indole-3-acetic acid (IAA), indole-3-butyric acid (IBA) and 1-naphthaleneacetic [corrected] acid (NAA) on mycelial growth of medicinal mushroom Phellinus linteus were investigated. Under the optimal IAA, IBA and NAA concentration of 1.0, 1.5 and 5.0 mg/l, the maximal mycelial diameter reached 8.6 +/- 0.4, 7.3 +/- 2.6 and 9.0 +/- 1.0 mm, respectively. The mycelial biomass and exopolysaccharide (EPS) production with addition of 5.0 mg/l NAA in a shake flask were 6.24 +/- 0.18 g/l at 168 h and 0.86 +/-0.01 g/l at 192 h, which were enhanced by 15.98 and 56.36% compared to the control, respectively. However, the molecular weight and infrared spectrum of EPS were coincident with the control. Results indicated that NAA at the proper concentration was beneficial in stimulating mycelial growth and EPS biosynthesis, whereas it could not alter the molecular structure of EPS.

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The effect of maltose on dextran yield and molecular weight distribution

Cited by 30 publications

References 18 publications

Effect of pH and Temperature on Enzyme Activity of Chitosanase Produced Under Solid Stated Fermentation by Trichoderma spp.

Effect of pH and Temperature on Enzyme Activity of Chitosanase Produced Under Solid Stated Fermentation by Trichoderma spp.

Dextransucrase production using cashew apple juice as substrate: effect of phosphate and yeast extract addition

Effects of phytohormones on mycelial growth and exopolysaccharide biosynthesis of medicinal mushroom Pellinus linteus

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