The effect of matrix stiffness on the chondrogenic differentiation of mesenchymal stem cells

Zhou, Yingjie; Qiu, Jingyi; Wan, Lingyun; Li, Juan

doi:10.1007/s10735-022-10094-6

Cited by 11 publications

(6 citation statements)

References 40 publications

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“…In terms of matrix stiffness, MSCs tend to be more chondrogenic in softer colloidal gels prepared from lower particle fraction which supports other studies where MSCs preferred moderately soft (in other words moderately stiff) matrix for chondrogenesis. [ 68,70–72 ] MSC responses analyzed with respect to the time‐dependent viscoelastic relaxations of CPN and APN gels show a higher level of chondrogenesis in moderately relaxing CPN gels compared to relatively higher relaxing APN gels. The effect of viscoelastic relaxations is highly variable depending on the cell type and its functional response and is not necessarily well defined.…”

Section: Discussionmentioning

confidence: 99%

Mechanomorphological Guidance of Colloidal Gel Regulates Cell Morphogenesis

Lin

Linares

Ramírez

et al. 2023

Macromolecular Bioscience

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Microstructural morphology of the extracellular matrix guides the organization of cells in 3D. However, current biomaterials‐based matrices cannot provide distinct spatial cues through their microstructural morphology due to design constraints. To address this, colloidal gels are developed as 3D matrices with distinct microstructure by aggregating ionic polyurethane colloids via electrostatic screening. Due to the defined orientation of interconnected particles, positively charged colloids form extended strands resulting in a dense microstructure whereas negatively charged colloids form compact aggregates with localized large voids. Chondrogenesis of human mesenchymal stem cells (MSCs) and endothelial morphogenesis of human endothelial cells (ECs) are examined in these colloidal gels. MSCs show enhanced chondrogenic response in dense colloidal gel due to their spatial organization achieved by balancing the cell–cell and cell–matrix interactions compared to porous gels where cells are mainly clustered. ECs tend to form relatively elongated cellular networks in dense colloidal gel compared to porous gels. Additionally, the role of matrix stiffness and viscoelasticity in the morphogenesis of MSCs and ECs are analyzed with respect to microstructural morphology. Overall, these results demonstrate that colloidal gels can provide spatial cues through their microstructural morphology and in correlation with matrix mechanics for cell morphogenesis.

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Section: Discussionmentioning

confidence: 99%

Mechanomorphological Guidance of Colloidal Gel Regulates Cell Morphogenesis

Lin

Linares

Ramírez

et al. 2023

Macromolecular Bioscience

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“…It has been shown that the matrix stiffness is a major stimulus to trigger chondrocyte differentiation. 54 As pure collagen hydrogels contract, the stiffness increases during cell culture to reach around tens of kPa. 55 So, BM-MSCs are in an appropriate environment to differentiate into chondrocytes.…”

Section: Discussionmentioning

confidence: 99%

Combining biomimetic collagen/hyaluronan hydrogels with discogenic growth factors promotes mesenchymal stroma cell differentiation into Nucleus Pulposus like cells

Okoro,

Frayssinet,

De Oliveira

et al. 2023

Biomater. Sci.

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“…Hydrogel Preparation: The polyacrylamide hydrogel was prepared as previously reported. [55] In brief, the coverslip was treated with 0.5% 3-(trimethoxysilyl)propylmethacrylate (Aladdin) and 0.5% glutaraldehyde (Aladdin) for 30 min, respectively. Subsequently, 40% acrylamide (Sigma-Aldrich) and 2% bis-acrylamide (Sigma-Aldrich) were mixed in different proportions, as shown in Table S1 (Supporting Information).…”

Section: Methodsmentioning

confidence: 99%

Effect of Hydrogel Matrix and Fluid Shear Stress on the Behavioral Regulation of Mesenchymal Stem Cells

Kang,

Wu,

et al. 2024

Small Structures

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Development of a micromodel that recapitulates multiple mechanical properties to mimic the complex mechanical microenvironment is crucial for cell‐based research. Herein, a microsystem combining structure of hydrogel matrix and acoustic streaming (AS) to mimic the cellular microenvironment is proposed, which can realize multiplex cellular mechanical cues, including matrix stiffness, fluid shear stress (FSS) generated by AS, and matrix roughness. The results demonstrate that the cell spreading area enlarges with the increase of matrix stiffness, and cell spreading is encouraged by integrin β1 cluster to polymerize actin when combines the hydrogel matrix with FSS. In addition, FSS has the influence on the roughness of the hydrogel, which further affects the cell morphology and mechanical properties, inducing mesenchymal stem cells (MSCs) differentiation into astrocytes rapidly. Meanwhile, cell migration is also enhanced by FSS stimulation, particularly, undifferentiated cells at 22 kPa hydrogel have the fastest migration speed, and change the movement model from contact inhibition to contact stimulation migration. Especially, matrix roughness and stiffness dominantly control of cell spreading and differentiation, whereas FSS affects cell migration. In conclusion, the microsystem in this work shows superior performance in regulating the spreading, differentiation, and migration of MSCs, which provides a new tool for cell‐microenvironment study.

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The effect of matrix stiffness on the chondrogenic differentiation of mesenchymal stem cells

Cited by 11 publications

References 40 publications

Mechanomorphological Guidance of Colloidal Gel Regulates Cell Morphogenesis

Mechanomorphological Guidance of Colloidal Gel Regulates Cell Morphogenesis

Combining biomimetic collagen/hyaluronan hydrogels with discogenic growth factors promotes mesenchymal stroma cell differentiation into Nucleus Pulposus like cells

Effect of Hydrogel Matrix and Fluid Shear Stress on the Behavioral Regulation of Mesenchymal Stem Cells

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