The effect of mesenchymal stem cells and sildenafil on flap viability in perforator‐based flaps for ischemia/reperfusion injury: An experimental study

Kelahmetoğlu, Osman; Demir, Rukiye; Ökten, Gülsen; Demir, Ahmet; Pınarlı, Ferda Alpaslan; Diraman, Emine

doi:10.1002/micr.22396

Cited by 7 publications

(9 citation statements)

References 28 publications

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“…Ischemia-reperfusion injury involves a complex oxidation process and is closely related to skin flap survival. [30][31][32] It involves many important factors, including various oxygen free radicals. In the early stages of oxidative stress, these radicals react with the lipids of cell membranes and proteins, triggering peroxidation and destroying cells and tissues.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Inducible Nitric Oxide Synthase on Multiterritory Perforator Flap Survival in Rats

Xianyao

Wang

Gao

et al. 2016

J reconstr Microsurg

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Inducible nitric oxide synthase (iNOS) plays an important role in vasodilation, angiogenesis, and ischemia-reperfusion injury. We investigated the effects of iNOS on the survival and choke vessels of multiterritory perforator flaps in rats. In this study, 84 rats were divided into two groups of 42 rats each and subjected to multiterritory perforator flap operations. Rats in group A received daily intraperitoneal doses of 100 mg per kg of aminoguanidine (AG) and rats in group B received daily intraperitoneal injections of the same volume of saline solution. On postoperative day 7, the surviving flap area was calculated as a percentage of the total flap dimensions using DP2-BSW software. The diameter and density of microvessels in the second choke zone of the flap were calculated from histology studies. The nitric oxide (NO) content was measured using NO concentration assay kits, and the levels of vascular endothelial growth factor (VEGF) and iNOS were assessed using western blotting. Superoxide dismutase (SOD) activity and malondialdehyde (MDA) content were measured using test kits. Laser Doppler imaging was used to evaluate flap perfusion in the second choke zone for 7 days after surgery. The flap survival area, diameter and density of microvessels, iNOS and VEGF levels, NO content, blood perfusion, and MDA content were significantly higher in the control group compared with the AG group, whereas SOD activity was significantly lower in the control group. iNOS has a beneficial effect on the survival of multiterritory perforator flaps.

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Section: Discussionmentioning

confidence: 99%

The Effect of Inducible Nitric Oxide Synthase on Multiterritory Perforator Flap Survival in Rats

Xianyao

Wang

Gao

et al. 2016

J reconstr Microsurg

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“…Due to the lack of literature analyzing biomarkers in diabetes with IR, we selected several biomarkers that may not be useful tools for assessing flap survival were selected. Some studies have investigated other biomarkers such as superoxide dismutase, and catalase, but the results have been inconclusive [21][22][23] .…”

Section: Discussionmentioning

confidence: 99%

The influence of adipocyte-derived stem cells (ASCs) on the ischemic epigastric flap survival in diabetic rats

et al. 2021

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“…I/R injury is linked with acute and chronic rejection and worse long-term outcomes in transplantation [52]. The ability of MSCs in mitigating reperfusion injury has been attributed to paracrine anti-inflammatory effects [53], with reduction in I/R injury in skin flaps [54,55] and in renal I/R injury [56,57]. In the latter study, MSCs but not conditioned medium were able to ameliorate I/R injured kidneys, which is in line with own findings where BM-MSCs but not conditioned medium were anti-inflammatory on activated endothelium in a critically ischaemic skin flap [53].…”

Section: Routes Of Administrationmentioning

confidence: 99%

Premise and promise of mesenchymal stem cell-based therapies in clinical vascularized composite allotransplantation

Schweizer

Gorantla

Plock

2015

Current Opinion in Organ Transplantation

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PURPOSE OF REVIEW Over the past decade, clinical vascularized composite allotransplantation (VCA) has enabled functional and quality of life restoration in a wide range of indications secondary to devastating tissue loss. However, the spectre of toxicity and long-term complications of chronic immunosuppression has curtailed the momentum of VCA. This study summarizes the literature evidence behind successful mesenchymal stem cell (MSC)-based cell therapies highlighting their multipronged immunomodulatory, restorative and regenerative characteristics with special emphasis towards VCA applications. RECENT FINDINGS Experimental and clinical studies in solid organs and VCA have confirmed that MSCs facilitate immunosuppression-free allograft survival or tolerance, stimulate peripheral nerve regeneration, attenuate ischaemia-reperfusion injury, and improve tissue healing after surgery. It has been hypothesized that MSC-induced long-term operational tolerance in experimental VCA is mediated by induction of mixed donor-specific chimerism and regulatory T-cell mechanisms. All these characteristics of MSCs could thus help expand the scope and clinical feasibility of VCA. SUMMARY Cellular therapies, especially those focusing on MSCs, are emerging in solid organ transplantation including VCA. Although some clinical trials have begun to assess the effects of MSCs in solid organ transplantation, much scientific domain remains uncharted, especially for VCA. Purpose of review Over the past decade, clinical vascularized composite allotransplantation (VCA) has enabled functional and quality of life restoration in a wide range of indications secondary to devastating tissue loss. However, the spectre of toxicity and long-term complications of chronic immunosuppression has curtailed the momentum of VCA. This study summarizes the literature evidence behind successful mesenchymal stem cell (MSC)-based cell therapies highlighting their multipronged immunomodulatory, restorative and regenerative characteristics with special emphasis towards VCA applications. Recent findingsExperimental and clinical studies in solid organs and VCA have confirmed that MSCs facilitate immunosuppression-free allograft survival or tolerance, stimulate peripheral nerve regeneration, attenuate ischaemia-reperfusion injury, and improve tissue healing after surgery. It has been hypothesized that MSCinduced long-term operational tolerance in experimental VCA is mediated by induction of mixed donorspecific chimerism and regulatory T-cell mechanisms. All these characteristics of MSCs could thus help expand the scope and clinical feasibility of VCA. SummaryCellular therapies, especially those focusing on MSCs, are emerging in solid organ transplantation including VCA. Although some clinical trials have begun to assess the effects of MSCs in solid organ transplantation, much scientific domain remains uncharted, especially for VCA.

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The effect of mesenchymal stem cells and sildenafil on flap viability in perforator‐based flaps for ischemia/reperfusion injury: An experimental study

Cited by 7 publications

References 28 publications

The Effect of Inducible Nitric Oxide Synthase on Multiterritory Perforator Flap Survival in Rats

The Effect of Inducible Nitric Oxide Synthase on Multiterritory Perforator Flap Survival in Rats

The influence of adipocyte-derived stem cells (ASCs) on the ischemic epigastric flap survival in diabetic rats

Premise and promise of mesenchymal stem cell-based therapies in clinical vascularized composite allotransplantation

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