The Effect of Methanol Concentration on the Extraction of Moringa Leaf (Moringa oleifera) and Papaya Fruit (Carica papaya) on Elastase and Hyaluronidase Installing Activities

Sugihartini, Nining; Nur, Mohamad Usman; Yuliani, Sapto

doi:10.3889/oamjms.2022.10302

Cited by 2 publications

(1 citation statement)

References 7 publications

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“…These results were consistent with those of researchers [23] who reported that 70% methanol solvent was the most effective solvent concentration to inhibit the enzymes elastase and hyaluronidase in moringa leaf methanol extracts. This destroys the aerobic bacteria in the egg shell.…”

Section: Egg Loss % At Different Period Of Storage Among Different Co...supporting

confidence: 93%

Effect of sprayed different concentrations of Moringa oleifera leaves extract on broiler breeder hatching eggs

Sulaiman

2023

JKAS

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This research aimed to study the impact of spraying different Concentrations of Moringa oleifera leaves Extract on Broiler Breeder Hatching Eggs. The results cleared that, at 18th day the egg weight ranged from 60.50 in the group treated with 5 % M. Water extract to 62.16 gm in control group. While, the egg losses observed in group 4 that, treated with 2 % M. water extract that was 20.56 % at 21st day. The higher hatchability % of total eggs showed in control group that was 69.70 %. While, the hatchability % of fertile eggs showed a higher percentage in group 5 that treated with 5 % M. Water extract that was 99.02 %. The higher percentage of dead embryo observed in control group that was 9.10 % and the lower level observed in the group 2 that treated with 2 % M. Alchole exteract that was 5.46 %. The total aerobic bacteria counts at 18th day of incubation showed a lower level in group 5 that treated with 5 % M. water extract that was 7.88. while, the activity of chicks and in abnormal chicks increased in group that treated with 5% M. Water extract that was 8.96 % and 2 % respectively. Overall, the higher concentration of Alchole extract of M. olifera , increased the weight of hatching eggs with a reduction in total egg losses,% of the eggs. While, the higher concentration of the water extract of M. olifera improved the hatchability % a reduction in total aerobic counts on the egg shell and improved chick quality and activity.

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Section: Egg Loss % At Different Period Of Storage Among Different Co...supporting

confidence: 93%

Effect of sprayed different concentrations of Moringa oleifera leaves extract on broiler breeder hatching eggs

Sulaiman

2023

JKAS

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The Potential of Tecoma stans (Linn.) Flower Extract as a Natural Antioxidant and Anti-Aging Agent for Skin Care Products

Wichayapreechar,

Prasansuklab,

Charoongchit

et al. 2024

Cosmetics

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Tecoma stans belongs to the Bignoniaceae family and possesses various pharmacological activities, including antimicrobial, anti-inflammatory, antifungal, antioxidant, and wound-healing activities. Although numerous studies have highlighted the beneficial effects of T. stans extracts, the impacts of different solvents on its biological activities, particularly its inhibitory effect on skin degradation enzymes (collagenase, elastase, and hyaluronidase assay), have not been reported. This study aims to explore the effects of different solvent extractions on the total phenolic and total flavonoid contents, antioxidant and anti-aging activities, and cytotoxicity. The most suitable extract was selected for incorporation into an anti-aging product. T. stans flowers were extracted using hexane, ethyl acetate, absolute ethanol, and deionized water through maceration. The aqueous extract yielded the highest extraction efficiency (40.73%), followed by absolute ethanol, ethyl acetate, and hexane. The phytochemical screening results revealed that all T. stans flower extracts contained phenolics, flavonoids, terpenoids, and alkaloids. Among the various solvents tested for T. stans flower extraction, absolute ethanol demonstrated the highest total phenolic content (24.10 ± 2.07 mg gallic acid equivalents (GAE)/g extract), followed by deionized water (20.83 ± 1.28 mg GAE/g extract). The highest total flavonoid content was observed in the ethyl acetate extract (205.11 ± 7.83 mg catechin equivalents (CE)/g extract), with ethanol showing a significantly lower concentration (140.67 ± 1.92 mg CE/g extract). In terms of antioxidant activity, the aqueous extract exhibited the most potent effects, with IC50 values of 0.600 ± 0.005 mg/mL for the DPPH• assay and 0.207 ± 0.001 mg/mL for the ABTS•+ assay. For anti-aging assays, the absolute ethanolic extract demonstrated the highest enzyme inhibition activity at 1 mg/mL, with collagenase, elastase, and hyaluronidase inhibition rates of 89.49% ± 2.96%, 94.61% ± 2.33%, and 82.56% ± 2.27%, respectively. Moreover, at a concentration of 50 µg/mL, the absolute ethanolic extract exhibited lower cytotoxicity, with human keratinocyte (HaCaT) cell viability of 78% ± 8.47%, which was significantly higher than that of the other extracts. An anti-aging gel containing 0.05% w/w of the ethanolic T. stans extract demonstrated physical and physicochemical stability during three months of storage at ambient temperatures, 4 °C, 45 °C, as well as after six cycles of heating/cooling tests. These findings suggest that the ethanolic extract of T. stans flower has potential as a safe and effective anti-aging agent for cosmeceutical products.

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The Effect of Methanol Concentration on the Extraction of Moringa Leaf (Moringa oleifera) and Papaya Fruit (Carica papaya) on Elastase and Hyaluronidase Installing Activities

Cited by 2 publications

References 7 publications

Effect of sprayed different concentrations of Moringa oleifera leaves extract on broiler breeder hatching eggs

Effect of sprayed different concentrations of Moringa oleifera leaves extract on broiler breeder hatching eggs

The Potential of Tecoma stans (Linn.) Flower Extract as a Natural Antioxidant and Anti-Aging Agent for Skin Care Products

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