The effect of micronscale anisotropic cross patterns on fibroblast migration

Jeon, Hojeong; Hidai, Hirofumi; Hwang, David J.; Healy, Kevin E.; Grigoropoulos, Costas P.

doi:10.1016/j.biomaterials.2010.01.103

Cited by 104 publications

(70 citation statements)

References 31 publications

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“…FAK is autophosphorylated upon integrin engagement to a target and activation by clustering of integrin pairs (Kirchner et al, 2006). Activation of FAK results in activation of the MAPK pathway, which leads to the activation of transcription factors (Juliano et al 2004).…”

Section: Discussionmentioning

confidence: 99%

“…Regulation of signaling pathways leads to the control of transcription factors. These transcription factors can, depending on other extracellular signals, activate a cascade of cellular processes, like proliferation, differentiation but also apoptosis (Juliano et al, 2004).…”

Section: Wwwecmjournalorg E Lamers Et Al the Influence Of Nanotopomentioning

confidence: 99%

“…In this context (directional) migration and correct positioning of cells on biomaterials are regarded as being very important for promoting wound healing and tissue regeneration. In order to achieve control over these phenomena, previous research has focused on the behavior of cells on substrates provided with organized micron scale topographies, which demonstrated that cellular morphology and migration behavior could be governed by such cues (Su et al, 2007;Kim et al, 2009;Jeon et al, 2010). However, the direct natural environment of a living cell is not (only) organized at the micrometer level, but mainly at the nanometric scale.…”

Section: Introductionmentioning

confidence: 99%

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The influence of nanoscale topographical cues on initial osteoblast morphology and migration

Lamers

Horssen²,

Riet³

et al. 2010

eCM

136

124

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The natural environment of a living cell is not only organized on a micrometer, but also on a nanometer scale. Mimicking such a nanoscale topography in implantable biomaterials is critical to guide cellular behavior. Also, a correct positioning of cells on biomaterials is supposed to be very important for promoting wound healing and tissue regeneration. The exact mechanism by which nanotextures can control cellular behavior are thus far not well understood and it is thus far unknown how cells recognize and respond to certain surface patterns, whereas a directed response appears to be absent on other pattern types. Focal adhesions (FAs) are known to be involved in the process of specific pattern recognition and subsequent response by cells. In this study, we used a high throughput screening "Biochip" containing 40 different nanopatterns to evaluate the influence of several nanotopographical cues like depth, width, (an)isotropy and spacing (ridge-groove ratio) on osteoblast behavior. Microscopical analysis and time lapse imaging revealed that an isotropic topography did not alter cell morphology, but it highly induced cell motility. Cells cultured on anisotropic topographies on the other hand, were highly elongated and aligned. Time-lapse imaging revealed that cell motility is highly dependent on the ridgegroove ratio of anisotropic patterns. The highest motility was observed on grooves with a ratio of 1:3, whereas the lowest motility was observed on ratios of 1:1 and 3:1. FA measurements demonstrated that FA-length decreased with increasing motility. From the study it can be concluded that osteoblast behavior is tightly controlled by nanometer surface features.

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Section: Discussionmentioning

confidence: 99%

Section: Wwwecmjournalorg E Lamers Et Al the Influence Of Nanotopomentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The influence of nanoscale topographical cues on initial osteoblast morphology and migration

Lamers

Horssen²,

Riet³

et al. 2010

eCM

136

124

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“…Increasing evidences show that surface patterning or alignment of nanofibers influences the guide cell growth direction and morphology [5,[16][17][18]. Zhang et al [18] show the contact guide of OP9 stroma cells on grooves patterned in PDMS surface.…”

Section: Introductionmentioning

confidence: 99%

Influence of crystallinity and fiber orientation on hydrophobicity and biological response of poly(l-lactide) electrospun mats

et al. 2012

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Royal Society of ChemistryAreias, A.; Ribeiro, C.; Sencadas, V.; Garcia Giralt, N.; Diez Perez, A.; Gómez Ribelles, JL.; Lanceros Mendez, S. (2012). Influence of crystallinity and fiber orientation on hydrophobicity and biological response of poly(L-lactide) electrospun mats. Soft Matter. 8 (21) AbstractPoly(L-lactide) electrospun mates have been produced with random and aligned fiber orientation and degrees of crystallinity from 0 up to nearly 50%. These two factors, fiber alignment and degree of crystallinity strongly affect the hydrophobicity of the samples, being this larger for the aligned fiber mats and for the fibers with higher degree of crystallinity. Whereas the first effect can be associated to a decrease in the degree of porosity the second should be related to variations in the fiber roughness at nanometric scale and an increase in fiber stiffness. Proliferation of human chondrocytes cultured in monolayer on these substrates is similar in both aligned and non-aligned amorphous mats. Crystallization of the aligned mats, on the other hand, nearly suppresses proliferation and the cells produce higher amounts of aggrecan, characteristic of the extracellular matrix of hyaline cartilage.

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“…This restriction has limited the understanding of the multidirectional aspects of cell-surface responses. 15 Within this context, the main objective of this work is to fabricate patterned and textured surfaces at microscale and nanoscale levels, respec-tively, to control cell-substrate interactions. As such, here we present a method for the fabrication of surface patterns of different dimensionality (1-D and 2-D), aimed at studying cell adhesion and migration.…”

Section: Introductionmentioning

confidence: 99%

Engineering of silicon surfaces at the micro- and nanoscales for cell adhesion and migration control

Torres-Costa¹,

Martínez-Muñoz²,

Sánchez-Vaquero³

et al. 2012

IJN

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Abstract:The engineering of surface patterns is a powerful tool for analyzing cellular communication factors involved in the processes of adhesion, migration, and expansion, which can have a notable impact on therapeutic applications including tissue engineering. In this regard, the main objective of this research was to fabricate patterned and textured surfaces at micron-and nanoscale levels, respectively, with very different chemical and topographic characteristics to control cell-substrate interactions. For this task, one-dimensional (1-D) and two-dimensional (2-D) patterns combining silicon and nanostructured porous silicon were engineered by ion beam irradiation and subsequent electrochemical etch. The experimental results show that under the influence of chemical and morphological stimuli, human mesenchymal stem cells polarize and move directionally toward or away from the particular stimulus. Furthermore, a computational model was developed aiming at understanding cell behavior by reproducing the surface distribution and migration of human mesenchymal stem cells observed experimentally.

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The effect of micronscale anisotropic cross patterns on fibroblast migration

Cited by 104 publications

References 31 publications

The influence of nanoscale topographical cues on initial osteoblast morphology and migration

The influence of nanoscale topographical cues on initial osteoblast morphology and migration

Influence of crystallinity and fiber orientation on hydrophobicity and biological response of poly(l-lactide) electrospun mats

Engineering of silicon surfaces at the micro- and nanoscales for cell adhesion and migration control

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