The effect of monoethanolamine as stabilizing agent in Uncaria gambir Roxb. mediated synthesis of silver nanoparticles and its antibacterial activity

Abstract

The development of an economic and eco-friendly approach of silver nanoparticles synthesis is an important issue due to its wide application, especially in biomedical applications. In present study, an eco-friendly method and size controlling is developed to synthesize silver nanoparticles using Uncaria gambir Roxb. and monoethanolamine (MEA) as bioreducing agent and stabilizing agent, respectively. The green synthesized silver nanoparticles are characterized using UV-Vis spectroscopy, X-ray diffraction (XRD), and transmission electron microscope (TEM). The silver nanoparticles are formed due to reducing of silver ion by catechin in Uncaria gambir Roxb. leaf extract which is visually recognized from the color change from pale yellow to grayish brown. The formation of silver nanoparticles is confirmed by UV-Vis spectroscopy analysis which provides SPR band range of 405-416 nm. The TEM analysis shows that spherical nanoparticles are formed with size range between 6-39 nm. The using of MEA with molar ratio to Ag þ 10/1 can maintain stability and reduce the particle size up to 40%. The result of XRD analysis shows 4 peaks referring to well-crystallized face-centred cubic silver nanoparticles. The prepared silver nanoparticle has exhibited good stability for more than 6 months. The synthesized silver nanoparticles exhibited good antibacterial activity against Staphylococcus aureus and Escherichia coli strain with a diameter of inhibition zone up to 36 mm. The synthesized silver nanoparticles also showed better activity against Escherichia coli than that of Staphylococcus aureus. These results show a good potential of Uncaria gambir Roxb. mediated silver nanoparticles to be developed in biomedical application.

Analisis Bibliometrik Penelitian Bio-Sintesis Nanopartikel Perak (NP-Ag) di Indonesia

“…Salah satu tumbuhan komoditas industri Indonesia yang telah dimanfaatkan sebagai sumber agen bio-reduksi dalam mensintesis NP-Ag adalah tumbuhan gambir (Uncaria gambir Roxb.) [8,9]. Lebih lanjut, rendahnya tingkat kestabilan dan aktifitas koloid NP-Ag masih menjadi kendala dalam aplikasi dilapangan.…”

Spinel ferrite of MnFe2O4 synthesized in Piper betle Linn extract media and its application as photocatalysts and antibacterial

“…Antibacterial activity of the samples was screened against pathogen bacteria, i.e. Staphylococcus aureus and Escherichia coli as following procedure [10,27] : Nutrient agar (NA) media was prepared by dissolving an amount of agar in distilled water, boiled, and sterilized in an autoclave at 121 C for 20 min, and then cooled until 45 C. Petri dishes for antibacterial assay were sterilized in an autoclave. Bacterial was subcultured in 5 mL of sterile distilled water for overnight.…”

Size controlled synthesis of well-distributed nano-silver on hydroxyapatite using alkanolamine compounds

“…Uncaria gambir Roxb. leaves were taken from the experimental garden, Andalas University, Padang, Indonesia and extracted using the method of Labanni et al [16]. Fresh leaves were washed, dehydrated by shade drying, and mashed up to obtain powder.…”

“…Several studies have reported that in the stabilizing mechanism of silver nanoparticles by alkanolamine compounds, an electrostatic interaction occurs between free electron pairs from nitrogen and positively charged AgNps, resulting in a protective monolayer. This monolayer then caps nanoparticles to avoid excessive fusion, hence controls the growth and size of nanoparticles [16,25,26]. It is assumed that hydroxyl groups of alkanolamine compound obstruct further interaction among silver nanoparticles.…”

“…In our previous studies, stable colloidal silver nanoparticles have been successfully synthesized by bio-reducing Ag + using Uncaria gambir Roxb. as a reducing agent in presence of MEA [16] and DEA [17] as a capping agent. Uncaria gambir Roxb.…”