The effect of multiple, microsecond electrical pulses on bacteria

Aly, R.E.; Joshi, R. P.; Stark, R.H.; Schoenbach, K.H.; Beebe, Stephen J.

doi:10.1109/ppps.2001.1001740

Cited by 4 publications

(2 citation statements)

References 8 publications

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“…The general range of electric field strength used in PEF treatment will be from 20 kV/cm to 80 kV/cm for getting good inactivation results. However, there are some studies which has given inactivation rates at lesser field values [25,26]. Since the novelty lies on the treatment chamber design, it was necessary to determine a suitable field value which should obtain effective inactivation rates using this chamber design.…”

Section: Membrane Filtration Methodsmentioning

confidence: 99%

High Voltage Pulsed Electric Field Application Using Titanium Electrodes for Bacterial Inactivation in Unpurified Water

2019

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Pulsed Electric Field (PEF) treatment is considered as nonthermal due to application of ultra short high voltage pulses in liquid foods to extend their shelf life. In today's world, water decontamination becomes extremely necessar y to safeguard people from health ailments. The objective of this work focusses on inactivation of naturally prevailing Escherichia coli and Fecal coliform bacteria in environmental water using titanium electrodes. In this study, the PEF treatment chamber was designed to be used for both static and continuous modes of treatment. Bipolar square wave pulses having 1 μs pulse width at a rise time of 160 ns and pulse repetition frequency between 48 to 50 Hz were used in this research. From the results, it was observed that titanium effectively inactivated both the microorganisms at a minimum treatment time of 60 seconds at 33.9℃ while conventional stainless steel required 120 seconds at a temperature of 40.1℃ under the same experimental conditions. Also, the relationship between treatment time and temperature remained linear despite the change in electric field. Results confirmed that (i) Titanium is more suitable in PEF for water decontamination due to its high reactivity than stainless steel (ii) Using titanium, complete ABSENCE of the two microorganisms could be possible in water at a nominal field strength of 24 kV/cm with much less temperature requirement.

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Section: Membrane Filtration Methodsmentioning

confidence: 99%

High Voltage Pulsed Electric Field Application Using Titanium Electrodes for Bacterial Inactivation in Unpurified Water

2019

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“…Their relaxation into a random distribution is determined by the viscosity of the medium. In experiments with Escherichia coli, it has been shown that reducing the time between pulses into the microsecond range increased the lethality of the pulses by more than a factor of two [46]. The scaling law, as shown in the example with platelet aggregation (Figure (4)), not only holds for primary effects on the membrane, such as electroporation, but also for secondary effects, and as long as they are intensityrelated, not necessarily linearly, to membrane charging, that means they are stimulated through membrane charging effects.…”

Section: Discussionmentioning

confidence: 99%

A scaling law for membrane permeabilization with nanopulses

Schoenbach

Joshi

Beebe

et al. 2009

IEEE Trans. Dielect. Electr. Insul.

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Experimental studies of plasma membrane permeabilization, caused by single, intense, submicrosecond square wave pulses, indicate that the product of electric field amplitude and pulse duration (the electrical impulse) can be considered a similarity or scaling factor. A model based on the hypothesis that the intensity of membrane permeabilization effects is linearly dependent on the electric charge transferred through the permeabilized membrane, provides results, which are consistent with the empirical observations. For multiple pulses, bioelectric effects caused by ultrashort pulses were found to scale with the square root of the pulse number. This square root dependence on the pulse number points to a statistical motion of cells between pulses with respect to the applied electric field, and can be explained using an extension of the random walk statistical results to random rotations. Besides membrane permeabilization, the scaling law has also been shown to hold for secondary bioelectric effects, which are caused by permeability changes in the plasma membrane or subcellular membranes.

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