The effect of mutagens on M1 population of black gram (Vigno mungo L. Hepper)

Ramya, B.; Nallathambi, G.; Ram, S. Ganesh

doi:10.5897/ajb2013.12785

Cited by 14 publications

(4 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, gamma radiation generally reduces the reproductive ability of the plant and increases the number of sterile pollen much more than the environmental effects. The results obtained were in accordance with the earlier reports of (Jain and Khandelwal, 2008) and (Ramya et al, 2014) in blackgram; (Khan et al, 2004) and (Singh and Singh, 2007) in greengram. The decrease in pollen fertility of rice after irradiation is considered to be due to chromosomal aberrations (Matsuo and Onozawa, 1961).…”

Section: Discussionsupporting

confidence: 92%

Improvement of restorer and maintainer lines in rice (Oryza sativa L.) by gamma radiation

Ghazy,

Hamad,

El-Kolly

2023

Egyptian Journal of Agricultural Research

View full text Add to dashboard Cite

Rice is a strictly self-pollinating crop. However, in hybrid rice seed production, an effective male sterility system is used to produce hybrid seed in bulk. In a hybrid rice system, the pollen grains of cytoplasmic male sterile (CMS) lines are sterile, and the female organ of the CMS depends on the fertile pollen released by the maintainer or restorer lines via out-crossing or cross-pollination to produce seeds. Floral traits and growth behavior of CMS and its corresponding maintainer and restorer lines are essential factors in hybrid rice seed production because they influence outcrossing or cross-pollination. Seeds of the 4 maintainer lines, or B lines, and the 5 restorer lines were treated with five gamma radiation doses: 0, 100, 200, 300, and 400 Gy, to study their mutagenic effect on the growth, yield, and floral traits of these rice genotypes, as well as to induce genetic variability for selecting plants with desirable characters. Results showed that all growth, yield, and floral traits studied in the M1 generation decreased steadily with increasing doses of gamma rays when compared to the control. The dose of 300 Gy recorded the highest mean values for some growth, yield, and floral traits studied in the M2 generation when compared to the control. The genotype-dose interaction was significant and highly significant for all growth, yield, and floral traits studied in M1 and M2 segregating generations except for anther width in the M1 generation, which indicated that the tested genotypes varied from treatment to treatment. The highest values for growth and yield traits were recorded when using Giza 178, Giza 182, and the IR 58025B mutant with 300 Gy of gamma rays in the M2 segregating generation. And the highest values for floral traits were recorded when using Giza 182 and the IR 70368B mutant with 300 Gy of gamma rays in the M2 segregating generation. The measurements of variation were, in general, higher in the treated plants at M2 generation. High heritability coupled with high genetic advance as percent of the mean was recorded for most studied traits, suggesting the improvement of these characters through simple phenotypic selection. Used irradiation by gamma-ray exhibited different genetic variability for the development of restorer and maintainer lines, such as desirable plant height, early heading, and high yield plants. Also, improving floral traits such as the length and width of anthers, percentage of pollen fertility, number of pollen grains per anther, total stigma length, and stigma width

show abstract

Section: Discussionsupporting

confidence: 92%

Improvement of restorer and maintainer lines in rice (Oryza sativa L.) by gamma radiation

Ghazy,

Hamad,

El-Kolly

2023

Egyptian Journal of Agricultural Research

View full text Add to dashboard Cite

show abstract

“…Similar effects on plant survival have been reported by Balai and Krishna (2009) in mungbean and Govardhan and Lal (2013) in field pea (Pisum sativum L.) and Rai et al (2013) in Linseed. Ramya et al (2014) carried out an experiment in black gram in M1 population and found the same results in pea. In the present study, the number of branches per plant of Sodium Azide administered M1 plants at maturity are presented in table 1.…”

Section: Resultsmentioning

confidence: 61%

Study of mutation induced by Sodium Azide in field pea (Pisum sativum L.)

Singh,

Jeberson,

Devi

et al. 2020

J. Current Opinion Crop Sci.,

View full text Add to dashboard Cite

The present study was conducted to determine the effect of Sodium Azide's sensitivity (0.1%, 0.3% and 0.5%) on three genotypes of field pea viz., Makhyatmubi, Makuchabi, and Rachna. In M1 generation, a dose-dependent decrease was observed for seed germination, seedling height and plant survival. The pollen fertility showed a negative dependence on Sodium Azide's dose as evident from proportionate decrease in fertility with the mutagen's increasing concentration in all the three varieties. The loweest pollen fertility was noted in a Makuchabi (83.76%) at 0.5% Sodium Azide. The four types of chlorophyll mutation (chlorina, xantha, albina, and viridis) were screened from the M2 population. Chlorina type of mutation was induced with the highest frequency at all the concentrations regardless of the variety studied. The greater concentration of Sodium Azide was observed to induce an extensive range of chlorophyll mutation in all the genotypes. However, the overall spectrum of chlorophyll mutation induced in field pea was in the following order; chlorina > viridis > xantha > albina. The magnitude of mutagenic effectiveness exposed a variety of response; it decreased with the increasing concentration of Sodium Azide on the varieties Makhyatmubi and Rachna, while on the other hand a rising trend of it was observed in the case of Makuchabi. Sodium Azide's efficiency for most of the treatment decreased with increase in the concentration in genotypes of Makhyatmubi and Rachna but for Makuchabi, it increased with the concentration in a decreasing trend.

show abstract

“…In previous finding showed that LD50 value as 250 Gy of gamma rays in balck gram var. TNAUCo(Bg)6 (Ramya et al, 2014), 450 Gy in green gram var. CO 6 and CO 8 (Rukesh et al, 2017), 375.52 Gy in green gram var.…”

Section: Resultsmentioning

confidence: 99%

Response of gamma irradiation on germination and seedling growth of green gram var. GAM 8

Thounaojam,

Patel,

Solanki

et al. 2024

ECJ

View full text Add to dashboard Cite

Being a pulse crop, green gram has huge scope in crop improvement in terms of productivity and other yield related parameters. Genetic variability is a preferable option for breeders in breeding programme for varietal development programme and others crop improvement aspect. The present investigation was framed to create the variability in mung bean var. GAM 8 during the seedling period through gamma (γ) irradiation. The significant variation was observed in germination percentage and it was found that dose dependent relationship between the germination rate and dose. Significantly minimum germination percentage (22.38 %) was observed in 700 Gy but optimal lethal dose (LD50) calculated through Probit analysis based on germination percentage was revealed at 540.26 Gy. Data revealed that γ irradiation had significantly reduced the seedling growth parameters such as shoot length (10.05 cm), shoot dry weight (19.68 mg), seedling length (13.90 cm), vigour index I (311.01) and II (509.01) was registered in 700 Gy while minimum root length (3.83 cm) was occurred in 600 Gy γ irradiation. Growth reduction (GR) 50 and 30 with respect to the seedling length was occurred respectively at 1093.79 and 1469.74 Gy. Therefore, this finding as the source of genetic variability would be used in future breeding and crop improvement programme like enhancement of yield potentiality and stress management in mung bean var. GAM 8.

show abstract

The effect of mutagens on M1 population of black gram (Vigno mungo L. Hepper)

Cited by 14 publications

References 4 publications

Improvement of restorer and maintainer lines in rice (Oryza sativa L.) by gamma radiation

Improvement of restorer and maintainer lines in rice (Oryza sativa L.) by gamma radiation

Study of mutation induced by Sodium Azide in field pea (Pisum sativum L.)

Response of gamma irradiation on germination and seedling growth of green gram var. GAM 8

Contact Info

Product

Resources

About