The effect of N-acetylcysteine and calcium hydroxide on TNF-α and TGF-β1 in lipopolysaccharide-activated macrophages

Karapinar, Senem Pinar; Ulum, Yeliz Z. Akkaya; Özçelik, Bahar; Buzoğlu, Hatice Doğan; Ceyhan, Deniz; Peynircioglu, B B; Aksoy, Yasemin

doi:10.1016/j.archoralbio.2016.03.017

Cited by 16 publications

(21 citation statements)

References 30 publications

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“…N-Acetylcysteine also modulates transcription activities through several pathways involving c-Fos/c-Jun, STAT, and cyclin inhibitors [ 39 ]. In oral inflammation, NAC prevents expression of lipopolysaccharide-induced proinflammatory cytokines such as interleukin-1 β (IL-1 β ), IL-6 and IL-8, tumor necrosis factor-alpha (TNF- α ), and transforming growth factor β (TGF- β ) in macrophages [ 40 ] and gingival fibroblasts [ 41 ]. Restorative resin materials may cause inflammatory responses by monocyte activation and changes in the levels of released cytokines.…”

Section: Anti-inflammatory Activitymentioning

confidence: 99%

“…Calcium hydroxide, on the other hand, decreases the bond strength of resin-based endodontic sealer to dentin [ 52 ] and is less effective against E. faecalis and Candida albicans [ 53 ]. Because NAC possesses anti-inflammatory effect on lipopolysaccharide-induced inflammatory responses [ 40 ] and analgesic property for relieving postendodontic pain that is comparable to the effect of ibuprofen [ 54 ], it has immense potential to be used as an alternate intracanal medicament in root canal treatment. Some research groups have combined additional components with NAC to achieve augmented or broad-spectrum antimicrobial applications.…”

Section: Antimicrobial Activitymentioning

confidence: 99%

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Biological Activities and Potential Oral Applications of N‐Acetylcysteine: Progress and Prospects

Pei

Liu

Yang

et al. 2018

Oxidative Medicine and Cellular Longevity

131

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N-Acetylcysteine (NAC), a cysteine prodrug and glutathione (GSH) precursor, has been used for several decades in clinical therapeutic practices as a mucolytic agent and for the treatment of disorders associated with GSH deficiency. Other therapeutic activities of NAC include inhibition of inflammation/NF-κB signaling and expression of proinflammatory cytokines. N-Acetylcysteine is also a nonantibiotic compound possessing antimicrobial property and exerts anticarcinogenic and antimutagenic effects against certain types of cancer. Recently, studies describing potentially important biological and pharmacological activities of NAC have stimulated interests in using NAC-based therapeutics for oral health care. The present review focused on the biological activities of NAC and its potential oral applications. The potential side effects of NAC and formulations for drug delivery were also discussed, with the intent of advancing NAC-associated treatment modalities in oral medicine.

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Section: Anti-inflammatory Activitymentioning

confidence: 99%

Section: Antimicrobial Activitymentioning

confidence: 99%

Biological Activities and Potential Oral Applications of N‐Acetylcysteine: Progress and Prospects

Pei

Liu

Yang

et al. 2018

Oxidative Medicine and Cellular Longevity

131

View full text Add to dashboard Cite

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“…Ca(OH)2 reduces TNF-a protein and mRNA level, initiates vascular and inflammatory cell migration and proliferation to handle and remove irritating agents. It will stimulate fibronectin gene expression, mineralization, inhibit bacterial LPS, so that it can induce tissue regeneration, including migration and proliferation of mesemchymal and endothelial pulp cells, followed by collagen formation [65,66]. Kasaj et al evaluated periodontal wound healing of non-surgical periodontal patients, and proved that the application of oily calcium hydroxide suspension (Osteoinductal) mediated the wound healing process, by leading superficial necrosis.…”

Section: Evaluation Of Epithelial Thickness On Proliferation Phase Ofmentioning

confidence: 99%

The Effect of Betel Quid Extract on Wound Healing Process in Male Wistar Rats (Rattus norvegicus L.)

Dewi

Fatonah

2019

AJAS

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Wound can be occured during dental treatment. In outline, there are several phases of wound healing; inflammatory phase, proliferative phase, and maturation phase. Various drugs in various forms are used to accelerate the healing process, but mostly they have side effects. Therefore, traditional medicine, such as betel quid consisted of betel leaf, areca nut, gambier, and calcium hydroxide, is developed. The aim of this study was to investigate the effect of wound healing process in male Wistar rats. A total of 30 male Wistar rats were taken and divided into 5 groups:Â Group 1, 2, and 3 (K1,K2, K3)Â were given 5%, 10% and 15% concentrations of betel quid extract oinments; Group 4 (K4) was positive controlÂ (hyaluronic acid 0.2% oinment); Group 5 (K5) was negative control (placebo oinment). One mm- diameter of lower lip mucosal wounds on rats were created by usingÂ cylinder diamond bur. The oinments were applied twice daily for 10 days. The number of netrophils on first and third day were measured and the thickness of ephitelium on 10 days were determined. All groups ofÂ betel quid extracts exhibited the reduction of the number of neutrophils on inflammatory phase. Group 3 shown as the highest effect and had no significant different with positive control. Betel quid extracts in all groups also improved epithelial thickness on proliferative phase, in which group II and group III had no significant different with positive control. Betel quid extract had effect on wound healing process in male Wistar rats due to its ability in supressing inflamation and in increasing reepithelization.

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“…N-acetylcysteine (NAC) has been proposed as a potential alternative therapeutic agent in root canal treatment (Karapinar et al 2016). NAC is an antioxidant mucolytic agent derivative of the amino acid Lcysteine (Atkuri et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Clinical influence of calcium hydroxide and N‐acetylcysteine on the levels of resolvins E1 and D2 in apical periodontitis

et al. 2020

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Aim To investigate the presence of resolvins E1 (RvE1) and D2 (RvD2) in teeth with primary endodontic infections and apical periodontitis, and to assess the influence of calcium hydroxide medication [Ca(OH) 2 ], in association with 2% chlorhexidine gel (2% CHX gel), and N-acetylcysteine (NAC) on the levels of RvE1 and RvD2 in periapical tissues. Methodology Thirty-six single-rooted teeth with primary endodontic infections and apical periodontitis were selected and randomly divided into three groups according to the medication: [Ca(OH) 2 ] + saline solution (SSL) [Ca(OH) 2 + SSL group] (n = 12), Ca (OH) 2 + 2% chlorhexidine gel [Ca(OH) 2 + 2% CHX gel group] (n = 12) and NAC [NAC group] (n = 12). Samples were collected from the periapical interstitial fluid at two different sampling times: before (S1) and after 14 days of intracanal medications (S2). Resolvins were measured using the enzyme-linked immunosorbent assay. Data were analysed using paired t-test, Wilcoxon test and Kruskal-Wallis test, followed by Dunn's post hoc test; all statistical tests were performed at a significance level of 5%. Results RvE1 and RvD2 were detected in 100% of the samples (36/36) at S1 and S2. Ca(OH) 2 medication did not increase the levels of RvE1 or RvD2 (both P > 0.05); however, NAC significantly increased the levels of RvE1 and RvD2 after 14 days of treatment (P < 0.05). Conclusions RvE1 and RvD2 were detected in periapical tissues from teeth with root canal infections. Moreover, calcium hydroxide medication did not increase the levels of resolvins in apical periodontitis. In contrast, the use of NAC intracanal medication significantly increased the levels of RvE1 and RvD2 after 14 days of treatment.

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The effect of N-acetylcysteine and calcium hydroxide on TNF-α and TGF-β1 in lipopolysaccharide-activated macrophages

Cited by 16 publications

References 30 publications

Biological Activities and Potential Oral Applications of N‐Acetylcysteine: Progress and Prospects

Biological Activities and Potential Oral Applications of N‐Acetylcysteine: Progress and Prospects

The Effect of Betel Quid Extract on Wound Healing Process in Male Wistar Rats (Rattus norvegicus L.)

Clinical influence of calcium hydroxide and N‐acetylcysteine on the levels of resolvins E1 and D2 in apical periodontitis

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