The effect of N-acetylcysteine in combination with vitamin C on the activity of ornithine decarboxylase of lung carcinoma cells — In vitro

Vanisree, Arambakkam Janardhanam; Shyamaladevi, Chennam Srinivasulu

doi:10.1016/j.lfs.2006.02.009

Cited by 5 publications

(4 citation statements)

References 21 publications

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“…Measurements of disintegrations per minute are made in a liquid scintillation spectrometer. The specific ODC activity is expressed as nmol CO 2 released/min/mg protein (Jones et al, 1972;Beaven et al, 1978;Smith and Marshall, 1988;Henley et al, 1996;Vanisree and Shyamaladevi, 2006;Schultz et al, 2020).…”

Section: Radiolabelingmentioning

confidence: 99%

“…There are several methods in the literature to assess ODC activity. The most used methods are based on the detection of radiolabeled CO 2 or radiolabeled DMFO linked to ODC, on the capture of CO 2 for the conversion of NADH into NAD with the use of Phosphoenolpyruvate Carboxylase Kobayashi (1963), Jones et al (1972), Beaven et al (1978), Smith and Marshall (1988), Henley et al (1996), Vanisree and Shyamaladevi (2006) (PEPC) and Malate Dehydrogenase (MDH) enzymes, on the chemiluminescence or colorimetric detection of H 2 O 2 produced by putrescine oxidation by amine oxidases, and in derivatization for analysis by high-performance liquid chromatography (HPLC) or mass spectrometry (MS) and fluorescence. However, we did not find a compilation of existing methods in the literature, which makes it difficult to choose the most appropriate method to assess ODC activity and screening for inhibitors.…”

Section: Introductionmentioning

confidence: 99%

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Analytical assays to evaluate enzymatic activity and screening of inhibitors for ornithine decarboxylase

Tinoco

Santos²,

Soares³

et al. 2022

Front. Anal. Sci.

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Ornithine decarboxylase (ODC) catalyzes the decarboxylation of ornithine to produce putrescine, the first step in the metabolism of polyamines (putrescine, spermidine, and spermine), which are essential growth factors in eukaryotic cells. ODC is active as a homodimer and depends on pyridoxal 5′-phosphate (PLP) as a cofactor. An increase in the concentration of polyamines has been associated with carcinogenesis. Therefore, there is much interest in identifying inhibitors of this pathway as potential chemotherapeutic and chemopreventive agents. The best-known inhibitor of mammalian ODC is α-difluoromethylornithine (DFMO), a highly selective compound that alkylates Cys-360 (a residue of the ODC active site). Although DFMO was initially developed for the treatment of cancer, the World Health Organization recommends its use in combination with nifurtimox for the treatment of human African trypanosomiasis. Considering the importance of ODC as a promising target for the treatment of various types of cancer and other infectious diseases, choosing the right method for screening potential inhibitors can help to accelerate the discovery of new drugs. Several methods for the determination of ODC activity are found in the literature. Among these, we can mention analysis with radioactive markers, colorimetric assays using auxiliary enzymes to detect CO2 or H2O2 release, chromatographic separations with putrescine derivatization, mass spectrometry, and spectroscopic techniques. In this review, the main analysis methods used will be described, highlighting their advantages and disadvantages, as well as identifying the most promising methods for high-throughput screening.

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Section: Radiolabelingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Analytical assays to evaluate enzymatic activity and screening of inhibitors for ornithine decarboxylase

Tinoco

Santos²,

Soares³

et al. 2022

Front. Anal. Sci.

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show abstract

“…In the absence of strong in vivo data, it would be unwise for us to speculate on whether the current combination will prove useful in the prevention of cancer. Given that NAC and EGCG are both widely used as dietary supplements, and have been studied individually for their anticancer and cancer preventive activities, we believe that further studies of the present combination are warranted [2,[25][26][27][28]. Structure of EGCG and NAC.…”

Section: Apoptosis Induction Was Determined By Measuring Tunel Positimentioning

confidence: 99%

N-Acetylcysteine enhances the lung cancer inhibitory effect of epigallocatechin-3-gallate and forms a new adduct

Lambert

Sang

Yang

2008

Free Radical Biology and Medicine

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The major tea polyphenol, (-)-epigallocatechin-3-gallate (EGCG), inhibits carcinogenesis in many in vivo models. Many potential mechanisms of action have been proposed based on cell line studies, including pro-oxidant activity. In the present study, we studied the effect of N-acetylcysteine (NAC) on the inhibitory effects of EGCG on lung cancer cell growth. We found that NAC (0 -2 mM) dosedependently enhanced the growth inhibitory activity of EGCG against murine and human lung cancer cells. The combination of NAC and EGCG caused an 8.8-fold increase in apoptosis in CL13 mouse and H1299 human lung cancer cells compared to treatment with either agent alone. Addition of 2 mM NAC increased the stability of EGCG in the presence of CL13 cells (t 1/2 = 8.5 h vs. 22.7 h). Intracellular levels of EGCG were increased 5.5-fold by the addition of 2 mM NAC. HPLC and LC-MS analyses of cell culture medium from CL13 cells treated with EGCG and NAC for 24 h revealed that EGCG-2′-NAC was time-dependently formed. This adduct was not formed in the absence of NAC. The present results show that under cell culture conditions, EGCG and NAC interact to form a previously unreported adduct, EGCG-2′-NAC, which may contribute to enhancement of EGCGmediated cell killing.

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“…N-Acetyl-L-cysteine (NAC), a thiol antioxidant that serves as a precursor of intracellular cysteine and glutathione (GSH), has been shown previously to inhibit DNA synthesis and cell proliferation of various cancer cells in vitro, including bladder cancer [3], small-cell lung cancer [4] pheochromocytoma [5], signet ring cell gastric cancer [6], prostate cancer [7], and pancreatic cancer [8]. In addition, NAC suppressed phenotypic features associated with cancer cells, such as migration and invasion in bladder cancer [3,9], melanoma, and Lewis lung carcinoma cells in vitro [10].…”

Section: Introductionmentioning

confidence: 99%

Molecular analysis of the inhibitory effect of N-acetyl-L-cysteine on the proliferation and invasiveness of pancreatic cancer cells

Mezencev

Wang²,

Xu³

et al. 2013

Anti-Cancer Drugs

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Preliminary studies have suggested that the reactive oxygen species (ROS) scavenger N-acetyl-L-cysteine (NAC) may be effective in inhibiting the growth of pancreatic cancer cells. In-depth cellular and molecular analyses were carried out to determine NAC's mode of action in inhibiting the growth of a well-characterized pancreatic cancer cell line (AsPC-1). Standardized assays were used to monitor cellular growth, apoptosis, levels of ROS, cellular senescence, migration, and invasiveness. Cell stiffness was measured using atomic force microscopy. Gene expression was monitored by quantitative PCR. NAC significantly inhibits the growth and metastatic potential of AsPC-1 cells by inducing cell-cycle arrest in G1 and subsequent cellular senescence and decreased invasiveness. These anticancer properties are associated with an unexpected increase in the intracellular concentrations of ROS. NAC does not decrease the susceptibility of AsPC-1 cells to the anticancer drugs gemcitabine, mitomycin C, and doxorubicin. NAC-induced changes in gene expression are consistent with the onset of mesenchymal-to-epithelial transition. In conclusion, our findings indicate that NAC induces an integrated series of responses in AsPC-1 cells that make it a highly promising candidate for development as a pancreatic cancer therapeutic.

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The effect of N-acetylcysteine in combination with vitamin C on the activity of ornithine decarboxylase of lung carcinoma cells — In vitro

Cited by 5 publications

References 21 publications

Analytical assays to evaluate enzymatic activity and screening of inhibitors for ornithine decarboxylase

Analytical assays to evaluate enzymatic activity and screening of inhibitors for ornithine decarboxylase

N-Acetylcysteine enhances the lung cancer inhibitory effect of epigallocatechin-3-gallate and forms a new adduct

Molecular analysis of the inhibitory effect of N-acetyl-L-cysteine on the proliferation and invasiveness of pancreatic cancer cells

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