The effect of N-(methoxy polyethylene glycol) p-hydroxymercuribenzamide, a polymeric sulfhydryl group reagent, on spermatozoan motility

Dawson, Marcia I.; Goerner, Richard N.; Tait, Anne

doi:10.1016/0010-7824(79)90088-x

Cited by 6 publications

(5 citation statements)

References 9 publications

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“…These data indicate Fig. While the present study was in progress, Dawson et al (1979) reported that the thiol reagent: p-chloromercuriphenylsulphonic acid (PCMPS) markedly inhibits motility of human sperm dispersed in diluted seminal plasma. Standard assay conditions were used with EP (*, 0.2 mg protein/ml; A, 2.0 mg protein/ml).…”

Section: Introductionmentioning

confidence: 57%

“…that spermatozoal outer surface -SH groups may not be essential for flagellar motility. While the present study was in progress, Dawson et al (1979) reported that the thiol reagent: p-chloromercuriphenylsulphonic acid (PCMPS) markedly inhibits motility of human sperm dispersed in diluted seminal plasma. It has been concluded that human sperm external surface -SH groups are involved in motility since the thiol reagent that has high affinity for -SH groups, is believed not to penetrate the sperm plasma membrane (Vansteveninck et al -1965).…”

Section: Figmentioning

confidence: 92%

“…Standard assay conditions were used with EP (*, 0.2 mg protein/ml; A, 2.0 mg protein/ml). In view of the recent finding from this laboratow that epididymd plasma (EP) possesses motility-factor protein(s) that may be necessary for sperm motility (Chaudhuri and Majumder -1983), it is not clear whether the observed inhibitory effect of the thiol reagents on sperm motility (Dawson et al -1979) is due to their interactions with the -SH groups of sperm surface or motility factors that may be present in the seminal plasma. Fig.…”

Section: Introductionmentioning

confidence: 99%

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Occurrence of -SH Containing Molecules on the Goat Sperm External Surface that are Essential for Flagellar Motility

Majumder

Chaudhuri

2009

Andrologia

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p-Chloromercuriphenylsulphonic acid (PCMPS), a thiol reagent which is believed not to enter the cells, strongly inhibits motility of freshly extracted goat caudaepididymal sperm preparations containing epididymal plasma (EP). The inhibitory action of PCMPS is not due to its interaction with the -SH groups of the surrounding EP-proteins. Washed cells when pretreated with low concentration of PCMPS (25 pM) for a short period (6 min) lost motility completely when assayed in presence of EP. PCMPSmediated inhibition of motility cannot be reversed with the addition of large excess of various-SH compounds. The data suggest that PCMPS binds with high affinity to the specific -SH group(s) of sperm ecto-sulfhydryl molecules to cause their inactivation irreversibly by altering their conformation and thereby destroys sperm motility. Nachweis von SH-Molekulen an der Ziegenbockspermatozoen-Oberflache als eine notwendige Voraussetzung fur die Motilitat der GeiRelZusammenfassung : p-chlormercuriphenylsulfonsiiure (PCMPS), ein Thiolreagens, welches nicht in die Zelle eindringt, hemmt die Motilitat von frisch extrahierten Ziegenbockspermatozoen-Priiparationen aus der Cauda epididymidis, die Nebenhodenplasma (E.P.) enthalten. Die Hemmwirkung von PCMPS ist nicht an eine Interaktion mit den SH-Crup pen der umgebenden EP-Proteine. Wenn die gewaschenen Zellen vorbehandelt wurden mit einer geringen Konzentration von PCMPS 125 pM) f k eine kune Periode von 6 Minuten, verloren sie ihre Motilitat vollstidig in Gegenwart von EP. PCMPS vermittelte Hemmung der Motilitat kann nicht umgekehrt werden mittels Zusatz von extremen Mengen an verschiedenen SH-Substanzen. Die Untersuchungbefunde legen es nahe, daO PCMPS mit einer hohen Affmitat an die spezifjichen SHGruppen der Spermatozoen Ekto-Sulfhydryl-Molekiiie um deren Inaktivierung irreversibel durch Vertinderung .ihrer Gestalt und dadurch die Spermatozoenmotilitat aufhebt.

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Section: Introductionmentioning

confidence: 57%

Section: Figmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

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Occurrence of -SH Containing Molecules on the Goat Sperm External Surface that are Essential for Flagellar Motility

Majumder

Chaudhuri

2009

Andrologia

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“…In addition to its effect of increasing structural stiffness of sperm flagella (Cornwall et al, 1988;Cornwall and Chang, 1990), diamide is known to decrease oxygen and Ca2+ uptake by mitochondria, an action that can be restored by dithioerythritol (Siliprandi et al, 1974). Other thiol-oxidising agents have also been shown to suppress Ca2+ uptake by bovine epididymal sperm (Breitbart and Lardy, 19871, oxygen uptake, as well as motility, of rabbit spermatozoa (Reyes et al, 1976) and motility of human sperm (Dawson et al, 1979). In contrast, reported effects of DTT on mammalian spermatozoa include an increase in sensitivity and response of demembranated sperm to localised stimulation by ATP (Yeung and Woolley, 1983), an increase in the percentage of demembranated sperm undergoing reactivation (Lindemann et al, 1988), and probably an increase in membrane stability as reflected by the inhibition of acrosome reaction and sperm-egg fusion induced by membrane mobility agents (Fleming et al, 1982).…”

Section: Influence Of Thiol-reducing or Oxidising Agents On Motility mentioning

confidence: 99%

Maturation of hamster epididymal sperm motility and influence of the thiol status of hamster and rat spermatozoa on their motility patterns

Yeung¹,

Oberländer²,

Cooper³

1994

Molecular Reproduction Devel

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A method for objective quantification of hamster sperm movement parameters as an indicator of maturation along the epididymis was established using a computerised system. Analysis of spermatozoa released into medium from five epididymal regions showed that the most drastic increases in percentage motility and curvilinear velocity (VCL) occurred from the distal corpus to the beginning of the proximal cauda and in straight-line velocity (VSL) from the beginning to a more distal site within the proximal cauda region. Both high osmolarity (400 mOsm/kg) and the thiol-oxidising agent diamide (10 microM) increased flagellar straightness of distal corpus spermatozoa, but VSL was increased only with the latter. The thiol-reducing agent dithiothreitol (DTT, 1mM) stimulated and maintained percentage motility and velocities of spermatozoa from the caput, stimulated only percentage motility of distal corpus sperm, but decreased velocities of those from the proximal cauda in prolonged incubation. In rats, diamide increased path straightness but not velocities of caput spermatozoa and yet caused immotility within 15 min, whereas DTT prolonged the maintenance of in vitro motility. The slight increases in kinematic parameters in the presence of DTT were enhanced by a 2-min preincubation with diamide. The finding that the effects of DTT and diamide were not compensatory suggests that the influence of the SH/S-S status on sperm movement is multifaceted, with decreasing sensitivity to stimulation upon sperm maturation.

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“…Disulfide bonding increases environment, sperm membranes are also exposed to gly-during epididymal transit on several species of sperm cosidases (Skudlarek and Orgebin-Crist, 1986;Chapman (Reyes et al, 1976;Huang et al, 1984;Sattayasai and and Killian, 1984), phospholipase (Thakkar et al, 1983), Panyim, 1982, and molecules containing sulfhydryl and phosphorylases (Noland et al, 1984; Mongkolsiri-groups are involved in the acquisition of motility during kieat and Chulavatnatol, 1984). Several mechanisms epididymal transit (Dawson et al, 1979; Majumder and may be operative in the modification of sperm during Chaudhuri, 1984). The addition of SMA4-like molecules epididymal transit.…”

Section: Functional Studies Of Wga Receptorsmentioning

confidence: 99%

Maturation antigen of the mouse sperm flagellum. I. Analysis of its secretion, association with sperm, and function

1988

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We report here recent findings on the sperm maturation antigen SMA4, which is secreted by holocrine cells of the distal caput epididymis and binds to the flagellar surface of mouse sperm during epididymal transit. Washed sperm from the caput and corpus epididymides of mice were examined by immunofluorescence and SDS-PAGE using wheat germ agglutinin, which binds specifically to SMA4 as a primary probe. Results indicate that sperm first exhibit WGA reactivity on their flagellae in the region of the distal caput, and that the appearance of WGA receptors is due to the binding of a 54-Kd glycoprotein (SMA4) to the cell surface. Extracts of epididymis containing SMA4 were tested for their ability to bind to the surfaces of caput and corpus sperm. Caput sperm surfaces bound SMA4 in a temperature-independent manner, and binding occurred in the presence of enzyme inhibitors, suggesting a nonenzymatic process. Biochemical studies revealed that SMA4 contains disulfide bonds which stabilize it on the sperm surface and restrict its mobility. Terminal carbohydrate residues of the molecule are sialic acids. The addition of SMA4 to caput sperm flagellae prevented tail-to-tail agglutination, normally seen when caput sperm are diluted into saline; and SMA4 was able to disperse clumps of agglutinated caput sperm. The data suggest that a primary function of SMA4 is to prevent tail-to-tail agglutination of sperm during storage in the epididymis.

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The effect of N-(methoxy polyethylene glycol) p-hydroxymercuribenzamide, a polymeric sulfhydryl group reagent, on spermatozoan motility

Cited by 6 publications

References 9 publications

Occurrence of -SH Containing Molecules on the Goat Sperm External Surface that are Essential for Flagellar Motility

Occurrence of -SH Containing Molecules on the Goat Sperm External Surface that are Essential for Flagellar Motility

Maturation of hamster epididymal sperm motility and influence of the thiol status of hamster and rat spermatozoa on their motility patterns

Maturation antigen of the mouse sperm flagellum. I. Analysis of its secretion, association with sperm, and function

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