The Effect of N-N-Dimethylformamide on the Membrane Characteristics of Canine Spermatozoa After Cryopreservation, and its Relationship With Post-Thaw Motility

Hernández‐Avilés, Camilo; Ruíz-Cristancho, Andrea; Vergara-Galván, Mónica; Zambrano-Varón, Jorge; Jiménez-Escobar, Claudia

doi:10.1016/j.tcam.2019.100372

Cited by 2 publications

(5 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It may be revealed from this study that there was no significant advantage using DMF to replace glycerol in cryopreservation of canine semen. Our observations are in agreement with that reported in canine (Futino et al 2008, Lopes et al 2009, Hernandez-Aviles et al 2020). However, it is suggested that 5% DMF in lactose extender could efficiently preserve post thaw quality of canine semen, with motile sperm values of 45.0% (Oliveira et al 2006).…”

Section: Comparative Evaluation Of Extenders Based On Sperm Attributessupporting

confidence: 94%

“…A study on canine semen indicated significant difference (P < 0.05) between glycerol and DMF with regard to subjective progressive motility, objective progressive motility, velocity average pathway and amplitude of lateral head, which also confirmed the efficiency of glycerol (Lopes et al, 2009) for cryopreservation of canine semen. Overall, sperm motility and membrane intactness/function were higher when glycerol was used as a cryoprotectant, as compared to DMF (P < 0.05) (Hernandez-Aviles et al, 2020).…”

Section: Comparative Evaluation Of Extenders Based On Sperm Attributesmentioning

confidence: 93%

“…Studies conducted in other animals, also have demonstrated a reduction in the sperm membrane intactness following semen cryopreservation using DMF as a cryoprotectant (Bezerra et al 2011 andForero-Gonzalez et al 2012). It may be assumed from poor results yielded using DMF as a cryoprotectant that the sperm plasma membrane from those species has a similar composition to the canine Comparing Glycerol with Dimethylformamide and Combination for Cryopreservation of Pug Dog Semen in Egg Yolk Plasma... sperm membranes and hypothesized that a common susceptibility to low-molecular weight cryoprotectants could exist in these species (Hernandez-Aviles et al 2020). The presence or absence of different transmembrane proteins with affinity for a specific cryoprotectant might account for the species-specific response to the effect of DMF or glycerol during freezing.…”

Section: Comparative Evaluation Of Extenders Based On Lipid Per Oxida...mentioning

confidence: 99%

See 2 more Smart Citations

Comparing Glycerol with Dimethylformamide and Combination for Cryopreservation of Pug Dog Semen in Egg Yolk Plasma Containing Extender

Sharma

Cheema

Pathania

2021

IJAR

View full text Add to dashboard Cite

Background: In order to find an alternative cryoprotectant for canine semen, different cryoprotectants, as dimethyl sulphoxide and ethylene glycol, dimethyle formamide either alone or in combination with glycerol have been evaluated on pooled semen of different breeds with different opinions. Methods: In the current study the effect of glycerol, DMF and their combination in Tris-citric acid-fructose egg yolk plasma extender on motility, viability, plasma membrane integrity (PMI), acrosome integrity (AI), inner mitochondrial membrane potential (IMMP), reactive oxygen species (ROS) and antioxidant enzymes in frozen-thaw semen of pug breed was compared. Result: Values for motility, viability, (PMI), AI were significantly (p less than 0.05) high in TCFEYP-G compared to TCFEYP-DMF and TCFEYP-G+DMF at post thaw. However, HIMMP and MIMMP/MDA were non-significantly (P greater than 0.05) high and low in TCFEYP-G compared to TCFEYP-DMF and TCFEYP- G + DMF at post thaw, respectively. MDA concentration was significantly (p less than 0.05) low in TCFEYP-G compared to TCFEYP-DMF and TCFEYP-G + TCFEYP-DMF extenders. However, H2O2, scavenging capacity of spermatozoa, cryopreserved in TCFEYP-G was significantly (p less than 0.05) higher than in TCFEYP-DMF and TCFEYP-G + TCFEYP-DMF. However, there was no significant (p greater than 0.05) difference in superoxide free radical scavenging activity and nitrite concentration of spermatozoa among the three extenders. Activity of GPX, SOD and Catalase was also significantly (p less than 0.05) higher in TCFEYP-G compared to TCFEYP-DMF and TCFEYP-G+DMF. However, values were reverse for GRE activity. This study concludes that glycerol is a healthier cryoprotectant to protect canine spermatozoa from cryo-injury during freezing-thawing process.

show abstract

Section: Comparative Evaluation Of Extenders Based On Sperm Attributessupporting

confidence: 94%

Section: Comparative Evaluation Of Extenders Based On Sperm Attributesmentioning

confidence: 93%

Section: Comparative Evaluation Of Extenders Based On Lipid Per Oxida...mentioning

confidence: 99%

See 1 more Smart Citation

Comparing Glycerol with Dimethylformamide and Combination for Cryopreservation of Pug Dog Semen in Egg Yolk Plasma Containing Extender

Sharma

Cheema

Pathania

2021

IJAR

View full text Add to dashboard Cite

show abstract

“…and N-N-dimethylformamide [22]. Glycerol is best at preserving the motility of cryopreserved spermatozoa after thawing.…”

Section: Extender For Canine Spermatozoamentioning

confidence: 99%

“…For canine spermatozoa, glycerol has been used as a penetrating intracellular cryoprotectant to preserve fertility after cryopreservation [ 16 ]. Other penetrating intracellular cryoprotectants include ethylene glycol [ 17 , 18 , 19 , 20 ], propylene glycol [ 20 ], dimethyl sulfoxide [ 16 ], methyl-formamide [ 20 , 21 ], dimethyl-formamide [ 20 , 21 ], methyl-acetamide [ 20 ], and N-N-dimethylformamide [ 22 ]. Glycerol is best at preserving the motility of cryopreserved spermatozoa after thawing.…”

Section: Extender For Canine Spermatozoamentioning

confidence: 99%

Assisted reproductive techniques for canines: preservation of genetic material in domestic dogs

2022

View full text Add to dashboard Cite

Assisted reproductive techniques (ARTs), such as artificial insemination, in vitro fertilization, and cryopreservation of gametes/zygotes, have been developed to improve breeding and reproduction of livestock and for the treatment of human infertility. Their widespread use has contributed to improvements in human health and welfare. However, in dogs, only artificial insemination using frozen semen is readily available as an ART to improve breeding and control genetic diversity. A recent priority in sperm cryopreservation is the development of alternatives to egg yolk, which is widely used as a component of the sperm extender. Egg yolk can vary in composition among batches and is prone to contamination by animal pathogens. The latter can be a problem for international exchange of cryopreserved semen. Low-density lipoprotein and skim milk are promising candidates for use as extenders, to ensure fertility after artificial insemination. Although not tested for its effects on fertility following artificial insemination, polyvinyl alcohol may also be a useful alternative to egg yolk as an extender. The development of cryopreservation techniques for canine embryos lags behind that for other mammals, including humans. However, given the success of non-surgical embryo transfer in 2011, studies have sought to refine this approach for practical use. Research on sperm cryopreservation has yielded satisfactory results. However, investigation of other approaches, such as cryopreservation of oocytes and gonadal tissues, remains insufficient. Techniques for the efficient induction of estrus may aid in the development of successful canine ARTs.

show abstract

The Effect of N-N-Dimethylformamide on the Membrane Characteristics of Canine Spermatozoa After Cryopreservation, and its Relationship With Post-Thaw Motility

Cited by 2 publications

References 51 publications

Comparing Glycerol with Dimethylformamide and Combination for Cryopreservation of Pug Dog Semen in Egg Yolk Plasma Containing Extender

Comparing Glycerol with Dimethylformamide and Combination for Cryopreservation of Pug Dog Semen in Egg Yolk Plasma Containing Extender

Assisted reproductive techniques for canines: preservation of genetic material in domestic dogs

Contact Info

Product

Resources

About