The Effect of Neuromuscular Blocking Agents on Isolated Human Intercostal Muscles

Creese, R.; Dillon, John; Marshall, John; Sabawala, Phiroze B.; Schneider, D. J.; Taylor, David S.; Zinn, D. E.

doi:10.1097/00132586-195806000-00003

Cited by 11 publications

(16 citation statements)

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“…Although injury may have elevated the mean mannitol ECV by some unknown amount, we think that the contribution of injury was small and therefore that our isolated human external intercostal preparations have a true ECV near that which we are reporting. Similar conclusions were reached by Creese et al (4) with isolated human external intercostal muscle.…”

Section: Introductionsupporting

confidence: 89%

“…We, as others (3,4,(12)(13)(14), have found that it can provide useful, reproducible electrophysiological, ion flux, and ion content data.…”

Section: Introductionmentioning

confidence: 74%

“…On extending these observations to man, we realized that cable parameters in isolated human external intercostal muscle have been reported for only seven fibers from two persons (3); ion content after several hours in physiological salt solution has been reported in only one other investigation (4); and no studies of the kinetics of exchange of K+ have been reported for any isolated human muscle.…”

Section: Introductionmentioning

confidence: 99%

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Cable parameters, sodium, potassium, chloride, and water content, and potassium efflux in isolated external intercostal muscle of normal volunteers and patients with myotonia congenita

Lipicky¹,

Bryant²,

Salmon³

1971

J. Clin. Invest.

150

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A B S T R A C T In isolated fiber bundles of external intercostal muscle from each of 13 normal volunteers and each of 6 patients with myotonia congenita, some or all of the following were measured: concentrations of Na+, K+, and Cl-, extracellular volume, water content, K+ efflux, fiber size, fiber cable parameters, and fiber resting potentials.Muscle from patients with myotonia congenita differed significantly (0.001 < P< 0.025) with respect to the following mean values (myotonia congenita vs. normal): the membrane resistance was greater (5729 vs. 2619 U. cm2), the internal resistivity was less (75.0 vs. 123.2 Q-cm), the water content was less (788.2 vs. 808.2 ml/kg wet weight), and the mean resting potential was greater (68 vs. 61 mv).No significant differences were found with respect to the following variables: K+ content (73.5 vs. 66.7 mEq/kg wet weight) and the calculated intracellular K+ concentration (215 vs. 191 mEq/liter fiber water), fiber capacitance (5.90 vs. 5.15 Mf/cm2), Na+ content (97.7 vs. 94.1 mEq/kg wet weight), 74.7 mEq/kg wet weight), mannitol extracellular volume (45.1 vs. 46.6 cc/100 g wet weight), and K+ efflux (23.2 vs. 21.5 moles X 10-12 cm-2.sec-1).These abnormalities of skeletal muscle in human myotonia congenita are like those of skeletal muscle in goats with hereditary myotonia. We tentatively conclude that a decreased Cl-permeability accounts for some of the abnormal electrical properties of skeletal muscle in myotonia congenita. We now report findings in isolated external intercostal muscle from patients with myotonia congenita and from normal volunteers. We have compared our human results with similar data we obtained in isolated external intercostal muscle from the goat (1, 2). METHODS Normal volunteers. Our normals are 13 males aged 21-33 yr with no evidence of neuropathy or myopathy. One (R. G.) had diabetes mellitus; another (T. P.) had probable pulmonary sarcoidosis (minimal pulmonary fibrosis on chest X-ray and scalene node biopsy positive for noncaseating granuloma). At the time of muscle biopsy, T. P. had no symptoms and his chest X-ray was clearer (without therapy) than 1 yr earlier. No volunteer had taken any medication for at least 5 days before the external intercostal muscle biopsy except for the patient with diabetes, who received insulin up to and including the day before biopsy. The remaining 11 subjects were healthy and the results of laboratory studies (chest X-ray, electrocardiogram, hemoglobin, hematocrit, white blood cell count and differential, urinalysis, serum urea nitrogen, fasting blood sugar, Wasserman, serum creatinine, serum ions [Na+, K+, Cl-], serum Ca and Mg, total serum proteins, serum alkaline phosphatase, serum glutamic oxalacetic transaminase, and creatinine phosphokinase) were normal. Biopsy techniques. Biopsies of the anterior border of external intercostal muscle (2.5-5 cm in length) were obtained under local anesthesia from the right eighth intercostal space. Preoperative medications were pentobarbital (from 100 to 200 mg), and morphin...

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Section: Introductionsupporting

confidence: 89%

“…We, as others (3,4,(12)(13)(14), have found that it can provide useful, reproducible electrophysiological, ion flux, and ion content data.…”

Section: Introductionmentioning

confidence: 74%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Cable parameters, sodium, potassium, chloride, and water content, and potassium efflux in isolated external intercostal muscle of normal volunteers and patients with myotonia congenita

Lipicky¹,

Bryant²,

Salmon³

1971

J. Clin. Invest.

150

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“…Decamethonium also has the initial excitatory effect, the subsequent (1) block and (D) depression of rapid onset, and the muscle depression is relieved in the continued presence of the drug in the bath, as in the case of maltoxin. Recovery from the muscle depression by decamethonium were also presented in the isolated human intercistal muscles (5). In our present experiments the nerve stimulation, on the other hand, remains ineff;c:ive when the muscle depression is releaved.…”

Section: Discussionsupporting

confidence: 68%

“…decamethonium, acetylcholine and suc cinylcholine were reported likewise to show a period of excitation preceding the neuro muscular block in mammalian muscles and to cause contracture in the rectus muscle of the frog (7-9). They were also shown to have a depressant effect on a variety of muscles (4,5,(10)(11)(12)(13)(14)(15). These similarities of the ac ion of maltoxin to that of depolarizing agents may suggest that the nature of the neuromuscular blocking action of maltoxin differs from that of d-tubocurarine and rather resembles that of decamethonium-like depolarizing agents.…”

Section: Discussionmentioning

confidence: 99%

The Paralyzing Action of Maltoxin, an Active Principle From Malt Rootlet, on the Frog Muscle

Urakawa

Deguchi

Ohkubo

1960

Japanese Journal of Pharmacology

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The resting membrane parameters of human intercostal muscle at low, normal, and high extracellular potassium

1984

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Membrane parameters at the respective resting potentials in low, normal, and high extracellular potassium solutions were determined in intercostal muscle fibers from 15 patients with no known neuromuscular disease. In synthetic interstitial fluid (normal potassium concentration 3.5 mmol/liter), we found the following mean values: resting membrane potential RP = -83.3 mV, space constant lambda = 2364 micron, fiber diameter d = 49.3 micron, fiber input resistance Rin = 795 k omega, specific membrane capacitance Cm = 4.7 muF/cm2, and specific membrane resistance Rm = 5970 omega X cm2. The specific membrane conductance was gm = 168 muS/cm2, 76% of it being chloride conductance, 24% being potassium conductance. The dependence of the membrane parameters on the extracellular potassium concentration followed the predictions by the constant field theory. There was no indication of active chloride transport. The resting membrane conductance decreased with temperature with a Q10 of 1.3. Excitability parameters were nearly independent of temperature between 37 and 27 degrees C.

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The Effect of Neuromuscular Blocking Agents on Isolated Human Intercostal Muscles

Cited by 11 publications

References 0 publications

Cable parameters, sodium, potassium, chloride, and water content, and potassium efflux in isolated external intercostal muscle of normal volunteers and patients with myotonia congenita

Cable parameters, sodium, potassium, chloride, and water content, and potassium efflux in isolated external intercostal muscle of normal volunteers and patients with myotonia congenita

The Paralyzing Action of Maltoxin, an Active Principle From Malt Rootlet, on the Frog Muscle

The resting membrane parameters of human intercostal muscle at low, normal, and high extracellular potassium

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